+ mkdir aih-tih-sc-505123-R1_B23WHYVLT4_1 + all_qc -bam aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-505123-R1_B23WHYVLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai 133296it [00:00, 1332859.58it/s] 274500it [00:00, 1379141.64it/s] 420270it [00:00, 1414985.80it/s] 561769it [00:00, 1412125.23it/s] 702983it [00:00, 1410918.42it/s] 844076it [00:00, 1337645.71it/s] 978504it [00:00, 1202853.50it/s] 1101269it [00:00, 1134966.01it/s] 1216733it [00:00, 1117429.99it/s] 1329710it [00:01, 1110093.59it/s] 1441518it [00:01, 1065936.41it/s] 1574449it [00:01, 1139644.37it/s] 1721009it [00:01, 1232700.53it/s] 1865143it [00:01, 1293075.02it/s] 1995632it [00:01, 1267991.73it/s] 2123313it [00:01, 1140047.85it/s] 2240125it [00:01, 1069792.05it/s] 2349505it [00:02, 1012216.23it/s] 2452564it [00:02, 982422.52it/s] 2551963it [00:02, 955930.84it/s] 2648255it [00:02, 945825.48it/s] 2743248it [00:02, 935395.64it/s] 2859963it [00:02, 1000239.40it/s] 2978484it [00:02, 1053054.34it/s] 3100543it [00:02, 1101584.22it/s] 3236497it [00:02, 1177080.56it/s] 3370860it [00:02, 1226121.71it/s] 3509558it [00:03, 1273679.39it/s] 3655452it [00:03, 1328751.45it/s] 3788680it [00:03, 1289608.06it/s] 3921458it [00:03, 1300739.92it/s] 4080164it [00:03, 1385064.88it/s] 4238113it [00:03, 1442626.68it/s] 4382738it [00:03, 1351557.83it/s] 4519270it [00:03, 1197521.97it/s] 4646816it [00:03, 1218094.04it/s] 4786103it [00:04, 1265909.66it/s] 4915207it [00:04, 1220938.05it/s] 5039215it [00:04, 1220579.54it/s] 5179320it [00:04, 1271650.47it/s] 5320787it [00:04, 1312848.64it/s] 5463838it [00:04, 1347001.81it/s] 5602419it [00:04, 1358391.08it/s] 5741887it [00:04, 1369053.34it/s] 5879225it [00:04, 1366517.08it/s] 6016179it [00:04, 1366384.69it/s] 6153601it [00:05, 1368686.79it/s] 6290619it [00:05, 1366437.61it/s] 6427368it [00:05, 1363269.08it/s] 6563769it [00:05, 1352498.19it/s] 6639198it [00:05, 1231497.50it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-505123-R1_B23WHYVLT4_1.md.bai + merge_qc -folders aih-tih-sc-505123-R1_B23WHYVLT4_1/aih-tih-sc-505123-R1_B23WHYVLT4_1.md -output aih-tih-sc-505123-R1_B23WHYVLT4_1/aih-tih-sc-505123-R1_B23WHYVLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-505123-R1_B23WHYVLT4_1/aih-tih-sc-505123-R1_B23WHYVLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-505123-R1_B23WHYVLT4_1/aih-tih-sc-505123-R1_B23WHYVLT4_1_full_basic_stats.tsv