[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (9024722) splicing reads. 219081 splicing junctions. 169981 known splicing junctions. 49100 novel splicing junctions.
sampling 10% (18049445) splicing reads. 252621 splicing junctions. 179991 known splicing junctions. 72630 novel splicing junctions.
sampling 15% (27074168) splicing reads. 272703 splicing junctions. 184935 known splicing junctions. 87768 novel splicing junctions.
sampling 20% (36098891) splicing reads. 286170 splicing junctions. 187864 known splicing junctions. 98306 novel splicing junctions.
sampling 25% (45123613) splicing reads. 295828 splicing junctions. 189833 known splicing junctions. 105995 novel splicing junctions.
sampling 30% (54148336) splicing reads. 303134 splicing junctions. 191272 known splicing junctions. 111862 novel splicing junctions.
sampling 35% (63173059) splicing reads. 308876 splicing junctions. 192341 known splicing junctions. 116535 novel splicing junctions.
sampling 40% (72197782) splicing reads. 313415 splicing junctions. 193149 known splicing junctions. 120266 novel splicing junctions.
sampling 45% (81222504) splicing reads. 317077 splicing junctions. 193803 known splicing junctions. 123274 novel splicing junctions.
sampling 50% (90247227) splicing reads. 320166 splicing junctions. 194327 known splicing junctions. 125839 novel splicing junctions.
sampling 55% (99271950) splicing reads. 322733 splicing junctions. 194757 known splicing junctions. 127976 novel splicing junctions.
sampling 60% (108296673) splicing reads. 324762 splicing junctions. 195098 known splicing junctions. 129664 novel splicing junctions.
sampling 65% (117321395) splicing reads. 326575 splicing junctions. 195386 known splicing junctions. 131189 novel splicing junctions.
sampling 70% (126346118) splicing reads. 328048 splicing junctions. 195628 known splicing junctions. 132420 novel splicing junctions.
sampling 75% (135370841) splicing reads. 329293 splicing junctions. 195839 known splicing junctions. 133454 novel splicing junctions.
sampling 80% (144395564) splicing reads. 330259 splicing junctions. 195996 known splicing junctions. 134263 novel splicing junctions.
sampling 85% (153420286) splicing reads. 331037 splicing junctions. 196102 known splicing junctions. 134935 novel splicing junctions.
sampling 90% (162445009) splicing reads. 331644 splicing junctions. 196195 known splicing junctions. 135449 novel splicing junctions.
sampling 95% (171469732) splicing reads. 332026 splicing junctions. 196250 known splicing junctions. 135776 novel splicing junctions.
sampling 100% (180494455) splicing reads. 332218 splicing junctions. 196277 known splicing junctions. 135941 novel splicing junctions.