[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3376ed-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	361330019
Known Splicing Events:	358348575
Partial Novel Splicing Events:	1843749
Novel Splicing Events:	1010891
Filtered Splicing Events:	126804

Total splicing  Junctions:	602087
Known Splicing Junctions:	275352
Partial Novel Splicing Junctions:	169540
Novel Splicing Junctions:	157195

===================================================================
Create BED file ...
Create Interact file ...