+ mkdir aih-tih-sc-0183f6-R1_B23WHYVLT4_1 + all_qc -bam aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-0183f6-R1_B23WHYVLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai 0it [00:00, ?it/s] 162553it [00:00, 1625411.42it/s] 333680it [00:00, 1675875.93it/s] 512993it [00:00, 1729389.45it/s] 685933it [00:00, 1729102.95it/s] 863769it [00:00, 1746844.27it/s] 1042045it [00:00, 1758899.72it/s] 1219703it [00:00, 1764532.54it/s] 1398923it [00:00, 1773319.68it/s] 1580984it [00:00, 1788082.38it/s] 1760607it [00:01, 1790557.19it/s] 1939663it [00:01, 1771751.96it/s] 2119735it [00:01, 1780483.16it/s] 2301163it [00:01, 1790653.63it/s] 2480411it [00:01, 1791118.98it/s] 2659545it [00:01, 1790768.75it/s] 2838638it [00:01, 1786859.23it/s] 3017480it [00:01, 1787311.09it/s] 3197140it [00:01, 1790001.13it/s] 3379966it [00:01, 1801460.06it/s] 3560119it [00:02, 1785370.94it/s] 3741404it [00:02, 1793541.67it/s] 3924063it [00:02, 1803268.01it/s] 4104416it [00:02, 1796146.29it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai 4284053it [00:02, 1786667.43it/s] 4462743it [00:02, 1765835.51it/s] 4639384it [00:02, 1744576.39it/s] 4813913it [00:02, 1720975.79it/s] 4993541it [00:02, 1743038.65it/s] 5176447it [00:02, 1768405.45it/s] 5357548it [00:03, 1780961.61it/s] 5538804it [00:03, 1790342.96it/s] 5719693it [00:03, 1795855.64it/s] 5899334it [00:03, 1789928.11it/s] 6081098it [00:03, 1798124.72it/s] 6261341it [00:03, 1799389.12it/s] 6441304it [00:03, 1788068.29it/s] 6621975it [00:03, 1793594.80it/s] 6804658it [00:03, 1803480.06it/s] 6985028it [00:03, 1791229.78it/s] 7164182it [00:04, 1780800.05it/s] 7342291it [00:04, 1775478.86it/s] 7520439it [00:04, 1777246.91it/s] 7698260it [00:04, 1777515.67it/s] 7876023it [00:04, 1773365.55it/s] 8053368it [00:04, 1759354.04it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai 8233098it [00:04, 1770617.52it/s] 8410945it [00:04, 1772882.42it/s] 8588254it [00:04, 1772517.01it/s] 8766267it [00:04, 1774775.10it/s] 8944949it [00:05, 1778360.63it/s] 9123020it [00:05, 1779047.04it/s] 9300931it [00:05, 1770851.50it/s] 9478029it [00:05, 1756737.55it/s] 9656775it [00:05, 1765860.95it/s] 9833389it [00:05, 1763653.60it/s] 10012967it [00:05, 1773226.94it/s] 10190308it [00:05, 1769303.66it/s] 10369016it [00:05, 1774547.18it/s] 10546483it [00:05, 1774322.48it/s] 10724416it [00:06, 1775805.59it/s] 10902003it [00:06, 1775798.76it/s] 11079587it [00:06, 1750123.22it/s] 11256720it [00:06, 1756389.05it/s] 11433742it [00:06, 1760415.81it/s] 11610375it [00:06, 1762164.67it/s] 11789098it [00:06, 1769639.78it/s] 11966965it [00:06, 1772325.68it/s] 12147044it [00:06, 1780799.87it/s] 12326784it [00:06, 1785759.74it/s] 12505372it [00:07, 1780871.40it/s] 12683470it [00:07, 1774367.60it/s] 12864347it [00:07, 1784628.34it/s] 13044332it [00:07, 1789165.99it/s] 13223259it [00:07, 1783766.63it/s] 13401646it [00:07, 1780834.65it/s] 13579737it [00:07, 1766564.63it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai 13756420it [00:07, 1764097.48it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai 13936111it [00:07, 1773857.38it/s] 14115927it [00:07, 1781096.22it/s] 14295121it [00:08, 1784318.63it/s] 14475676it [00:08, 1790651.86it/s] 14654752it [00:08, 1774651.77it/s] 14834460it [00:08, 1781315.52it/s] 15016767it [00:08, 1793760.33it/s] 15197923it [00:08, 1799063.20it/s] 15377852it [00:08, 1790591.96it/s] 15556934it [00:08, 1773173.70it/s] 15734298it [00:08, 1771072.54it/s] 15911436it [00:08, 1759284.74it/s] 16087395it [00:09, 1756324.07it/s] 16263047it [00:09, 1753605.98it/s] 16438854it [00:09, 1754923.71it/s] 16442787it [00:09, 1774232.94it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md.bai + merge_qc -folders aih-tih-sc-0183f6-R1_B23WHYVLT4_1/aih-tih-sc-0183f6-R1_B23WHYVLT4_1.md -output aih-tih-sc-0183f6-R1_B23WHYVLT4_1/aih-tih-sc-0183f6-R1_B23WHYVLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-0183f6-R1_B23WHYVLT4_1/aih-tih-sc-0183f6-R1_B23WHYVLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-0183f6-R1_B23WHYVLT4_1/aih-tih-sc-0183f6-R1_B23WHYVLT4_1_full_basic_stats.tsv