Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2f/932dc939b35f32e48255b5efa6681e/FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_genome.fa
Downloading: s3://natera-platform-sandbox/pipeline-resources/Homo_sapiens_assembly38.dbsnp138.vcf.gz
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2e/1c0e35bb967705cb96da5af65cac26/.command.sh
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2f/932dc939b35f32e48255b5efa6681e/FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bam
Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_genome.dict
Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/gatk4/dbsnp_hg38_contam_sites.bed
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2e/1c0e35bb967705cb96da5af65cac26/.command.run
Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai
Downloading: s3://natera-platform-sandbox/pipeline-resources/beds/twist/Twist_rna_exome_AND_fusion_hg38.bed
Downloading: s3://natera-platform-sandbox/pipeline-resources/Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi
==> STAGING COMPLETE (11 inputs)
+ mkdir FFPE_HD789_01_RNA_13_B23WHYVLT4_1
+ all_qc -bam FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder FFPE_HD789_01_RNA_13_B23WHYVLT4_1 -reference ref_genome.fa -region chr20
mkdir -p FFPE_HD789_01_RNA_13_B23WHYVLT4_1/FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md/
cat Twist_rna_exome_AND_fusion_hg38.bed | cut -f 1-3 | bedtools sort -g ref_genome.fa.fai | bedtools merge > FFPE_HD789_01_RNA_13_B23WHYVLT4_1/FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md/full_bed.bed
seqtool depth -bam FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bam -bed FFPE_HD789_01_RNA_13_B23WHYVLT4_1/FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md/full_bed.bed -threads 4 -bam_threads 2 | bgzip -c > FFPE_HD789_01_RNA_13_B23WHYVLT4_1/FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md/FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.depth.gz
saturation_plot -bams FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bam -region chr20 -output FFPE_HD789_01_RNA_13_B23WHYVLT4_1/FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md/saturation_plot.png -threads 1
seqtool saturation_metric -at 1000000 -region chr20 -bam_threads 1 FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bam > FFPE_HD789_01_RNA_13_B23WHYVLT4_1/FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md/saturation_qc.tsv
fragment_size -bams FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bam -region chr20 -output_prefix FFPE_HD789_01_RNA_13_B23WHYVLT4_1/FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md/fragsize -threads 1
samtools mpileup -B --min-MQ 60 --min-BQ 30 -f ref_genome.fa -l dbsnp_hg38_contam_sites.bed FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bam > FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.mpileup && contamination -input FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.mpileup -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz > FFPE_HD789_01_RNA_13_B23WHYVLT4_1/FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md/contamination.tsv
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[mpileup] 1 samples in 1 input files
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
/opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81.
import pkg_resources
/opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81.
import pkg_resources
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
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[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai
[W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23WHYVLT4_1.md.bai