[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3924cb-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	345102544
Known Splicing Events:	342128516
Partial Novel Splicing Events:	1597374
Novel Splicing Events:	1270961
Filtered Splicing Events:	105693

Total splicing  Junctions:	570528
Known Splicing Junctions:	274798
Partial Novel Splicing Junctions:	159749
Novel Splicing Junctions:	135981

===================================================================
Create BED file ...
Create Interact file ...