[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1d9134-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (14817897) splicing reads. 240808 splicing junctions. 179650 known splicing junctions. 61158 novel splicing junctions.
sampling 10% (29635795) splicing reads. 292822 splicing junctions. 192606 known splicing junctions. 100216 novel splicing junctions.
sampling 15% (44453692) splicing reads. 330165 splicing junctions. 199783 known splicing junctions. 130382 novel splicing junctions.
sampling 20% (59271590) splicing reads. 359773 splicing junctions. 204484 known splicing junctions. 155289 novel splicing junctions.
sampling 25% (74089488) splicing reads. 384262 splicing junctions. 207988 known splicing junctions. 176274 novel splicing junctions.
sampling 30% (88907385) splicing reads. 405176 splicing junctions. 210711 known splicing junctions. 194465 novel splicing junctions.
sampling 35% (103725283) splicing reads. 422858 splicing junctions. 212857 known splicing junctions. 210001 novel splicing junctions.
sampling 40% (118543180) splicing reads. 438085 splicing junctions. 214544 known splicing junctions. 223541 novel splicing junctions.
sampling 45% (133361078) splicing reads. 451533 splicing junctions. 216052 known splicing junctions. 235481 novel splicing junctions.
sampling 50% (148178976) splicing reads. 463571 splicing junctions. 217322 known splicing junctions. 246249 novel splicing junctions.
sampling 55% (162996873) splicing reads. 474042 splicing junctions. 218363 known splicing junctions. 255679 novel splicing junctions.
sampling 60% (177814771) splicing reads. 483292 splicing junctions. 219312 known splicing junctions. 263980 novel splicing junctions.
sampling 65% (192632668) splicing reads. 491500 splicing junctions. 220053 known splicing junctions. 271447 novel splicing junctions.
sampling 70% (207450566) splicing reads. 498489 splicing junctions. 220669 known splicing junctions. 277820 novel splicing junctions.
sampling 75% (222268464) splicing reads. 504677 splicing junctions. 221239 known splicing junctions. 283438 novel splicing junctions.
sampling 80% (237086361) splicing reads. 510002 splicing junctions. 221665 known splicing junctions. 288337 novel splicing junctions.
sampling 85% (251904259) splicing reads. 514574 splicing junctions. 222038 known splicing junctions. 292536 novel splicing junctions.
sampling 90% (266722156) splicing reads. 518432 splicing junctions. 222386 known splicing junctions. 296046 novel splicing junctions.
sampling 95% (281540054) splicing reads. 521526 splicing junctions. 222645 known splicing junctions. 298881 novel splicing junctions.
sampling 100% (296357952) splicing reads. 523923 splicing junctions. 222871 known splicing junctions. 301052 novel splicing junctions.