[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3924cb-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (17249516) splicing reads. 261350 splicing junctions. 185063 known splicing junctions. 76287 novel splicing junctions.
sampling 10% (34499033) splicing reads. 319956 splicing junctions. 197858 known splicing junctions. 122098 novel splicing junctions.
sampling 15% (51748549) splicing reads. 361885 splicing junctions. 204568 known splicing junctions. 157317 novel splicing junctions.
sampling 20% (68998066) splicing reads. 394358 splicing junctions. 209045 known splicing junctions. 185313 novel splicing junctions.
sampling 25% (86247583) splicing reads. 421513 splicing junctions. 212407 known splicing junctions. 209106 novel splicing junctions.
sampling 30% (103497099) splicing reads. 444074 splicing junctions. 214890 known splicing junctions. 229184 novel splicing junctions.
sampling 35% (120746616) splicing reads. 463209 splicing junctions. 216862 known splicing junctions. 246347 novel splicing junctions.
sampling 40% (137996132) splicing reads. 479820 splicing junctions. 218483 known splicing junctions. 261337 novel splicing junctions.
sampling 45% (155245649) splicing reads. 494288 splicing junctions. 219799 known splicing junctions. 274489 novel splicing junctions.
sampling 50% (172495166) splicing reads. 507031 splicing junctions. 221032 known splicing junctions. 285999 novel splicing junctions.
sampling 55% (189744682) splicing reads. 518213 splicing junctions. 222005 known splicing junctions. 296208 novel splicing junctions.
sampling 60% (206994199) splicing reads. 527986 splicing junctions. 222856 known splicing junctions. 305130 novel splicing junctions.
sampling 65% (224243715) splicing reads. 536579 splicing junctions. 223553 known splicing junctions. 313026 novel splicing junctions.
sampling 70% (241493232) splicing reads. 544066 splicing junctions. 224153 known splicing junctions. 319913 novel splicing junctions.
sampling 75% (258742749) splicing reads. 550533 splicing junctions. 224651 known splicing junctions. 325882 novel splicing junctions.
sampling 80% (275992265) splicing reads. 556016 splicing junctions. 225089 known splicing junctions. 330927 novel splicing junctions.
sampling 85% (293241782) splicing reads. 560747 splicing junctions. 225469 known splicing junctions. 335278 novel splicing junctions.
sampling 90% (310491298) splicing reads. 564687 splicing junctions. 225776 known splicing junctions. 338911 novel splicing junctions.
sampling 95% (327740815) splicing reads. 567849 splicing junctions. 226024 known splicing junctions. 341825 novel splicing junctions.
sampling 100% (344990332) splicing reads. 570354 splicing junctions. 226234 known splicing junctions. 344120 novel splicing junctions.