+ mkdir aih-tih-sc-be4b28-R1_B23WHYVLT4_1 + all_qc -bam aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-be4b28-R1_B23WHYVLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai 0it [00:00, ?it/s] 117935it [00:00, 1179190.87it/s] 248811it [00:00, 1255351.66it/s] 378060it [00:00, 1272266.53it/s] 515532it [00:00, 1312473.99it/s] 646779it [00:00, 1202041.42it/s] 785083it [00:00, 1260019.95it/s] 914084it [00:00, 1269409.08it/s] 1044713it [00:00, 1280787.46it/s] 1173445it [00:00, 1134018.16it/s] 1311942it [00:01, 1203872.83it/s] 1450945it [00:01, 1256823.52it/s] 1589355it [00:01, 1293620.98it/s] 1720623it [00:01, 1283272.26it/s] 1854354it [00:01, 1299035.09it/s] 1987958it [00:01, 1309905.06it/s] 2122106it [00:01, 1319229.39it/s] 2256833it [00:01, 1327537.56it/s] 2391770it [00:01, 1333880.50it/s] 2525420it [00:01, 1329396.91it/s] 2662621it [00:02, 1341961.52it/s] 2799964it [00:02, 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6586744it [00:05, 983656.83it/s] 6725462it [00:05, 1092383.47it/s] 6865199it [00:05, 1177175.12it/s] 7005299it [00:05, 1240747.75it/s] 7148216it [00:05, 1295148.89it/s] 7286359it [00:05, 1320319.21it/s] 7431180it [00:06, 1357946.98it/s] 7573578it [00:06, 1377341.91it/s] 7712058it [00:06, 1376723.96it/s] 7850252it [00:06, 1377300.41it/s] 7988348it [00:06, 1369638.55it/s] 8130816it [00:06, 1385980.12it/s] 8271013it [00:06, 1390723.39it/s] 8410315it [00:06, 1391383.05it/s] 8549553it [00:06, 1384395.14it/s] 8688069it [00:06, 1373380.69it/s] 8825475it [00:07, 1359884.43it/s] 8961529it [00:07, 1347251.39it/s] 9096311it [00:07, 1342723.26it/s] 9230619it [00:07, 1338365.10it/s] 9364478it [00:07, 1337034.82it/s] 9498196it [00:07, 1314074.84it/s] 9629683it [00:07, 1288744.20it/s] 9759986it [00:07, 1292889.16it/s] 9889373it [00:07, 1292779.04it/s] 10018719it [00:08, 1289648.46it/s] 10148873it [00:08, 1293151.45it/s] 10278224it [00:08, 1290521.37it/s] 10407301it [00:08, 1269731.82it/s] 10540943it [00:08, 1289386.79it/s] 10685685it [00:08, 1336230.20it/s] 10825934it [00:08, 1355899.21it/s] 10966241it [00:08, 1369806.77it/s] 11009920it [00:08, 1257642.65it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md.bai + merge_qc -folders aih-tih-sc-be4b28-R1_B23WHYVLT4_1/aih-tih-sc-be4b28-R1_B23WHYVLT4_1.md -output aih-tih-sc-be4b28-R1_B23WHYVLT4_1/aih-tih-sc-be4b28-R1_B23WHYVLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-be4b28-R1_B23WHYVLT4_1/aih-tih-sc-be4b28-R1_B23WHYVLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-be4b28-R1_B23WHYVLT4_1/aih-tih-sc-be4b28-R1_B23WHYVLT4_1_full_basic_stats.tsv