+ mkdir aih-tih-sc-176637-R1_B23WHYVLT4_1 + all_qc -bam aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-176637-R1_B23WHYVLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai 134158it [00:00, 1341287.07it/s] 284562it [00:00, 1436973.97it/s] 434398it [00:00, 1464972.65it/s] 587204it [00:00, 1489851.47it/s] 741702it [00:00, 1509707.50it/s] 895969it [00:00, 1520895.10it/s] 1049850it [00:00, 1526730.76it/s] 1202524it [00:00, 1526268.48it/s] 1355151it [00:00, 1525829.40it/s] 1508479it [00:01, 1528111.18it/s] 1668332it [00:01, 1549640.92it/s] 1823297it [00:01, 1490826.51it/s] 1972849it [00:01, 1464859.56it/s] 2126296it [00:01, 1485113.65it/s] 2275130it [00:01, 1484312.82it/s] 2423864it [00:01, 1485088.80it/s] 2572531it [00:01, 1481463.29it/s] 2723315it [00:01, 1489102.36it/s] 2872309it [00:01, 1469484.92it/s] 3019362it [00:02, 1440216.85it/s] 3163962it [00:02, 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1325495.38it/s] 7309177it [00:05, 1311125.47it/s] 7440509it [00:05, 1289151.30it/s] 7569612it [00:05, 1284548.47it/s] 7707122it [00:05, 1311050.60it/s] 7838377it [00:05, 1306757.22it/s] 7969154it [00:05, 1290560.82it/s] 8098306it [00:05, 1271418.80it/s] 8225546it [00:05, 1260907.81it/s] 8351702it [00:06, 1242872.87it/s] 8476055it [00:06, 1229314.19it/s] 8599033it [00:06, 1192985.27it/s] 8718517it [00:06, 1185205.85it/s] 8837150it [00:06, 1158870.08it/s] 8953169it [00:06, 1157946.83it/s] 9069053it [00:06, 1157337.08it/s] 9185069it [00:06, 1158143.50it/s] 9300928it [00:06, 1128556.48it/s] 9413945it [00:07, 1120952.70it/s] 9526145it [00:07, 1115820.56it/s] 9637793it [00:07, 1103390.88it/s] 9755987it [00:07, 1126397.48it/s] 9884245it [00:07, 1172480.34it/s] 10001631it [00:07, 1156265.81it/s] 10119948it [00:07, 1164165.16it/s] 10236471it [00:07, 1157337.56it/s] 10352281it [00:07, 1153468.88it/s] 10464554it [00:07, 1317270.32it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-176637-R1_B23WHYVLT4_1.md.bai + merge_qc -folders aih-tih-sc-176637-R1_B23WHYVLT4_1/aih-tih-sc-176637-R1_B23WHYVLT4_1.md -output aih-tih-sc-176637-R1_B23WHYVLT4_1/aih-tih-sc-176637-R1_B23WHYVLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-176637-R1_B23WHYVLT4_1/aih-tih-sc-176637-R1_B23WHYVLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-176637-R1_B23WHYVLT4_1/aih-tih-sc-176637-R1_B23WHYVLT4_1_full_basic_stats.tsv