+ mkdir aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1 + all_qc -bam aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai 0it [00:00, ?it/s] 142887it [00:00, 1428803.23it/s] 293495it [00:00, 1474231.16it/s] 446616it [00:00, 1500222.88it/s] 606405it [00:00, 1538735.56it/s] 776272it [00:00, 1596385.77it/s] 947669it [00:00, 1636343.90it/s] 1119866it [00:00, 1664317.35it/s] 1291176it [00:00, 1679824.56it/s] 1460406it [00:00, 1683703.63it/s] 1630045it [00:01, 1687598.20it/s] 1801871it [00:01, 1696846.33it/s] 1975227it [00:01, 1707986.19it/s] 2147351it [00:01, 1711979.21it/s] 2318549it [00:01, 1711504.22it/s] 2490676it [00:01, 1714393.54it/s] 2662611it [00:01, 1715863.49it/s] 2834198it [00:01, 1715786.00it/s] 3006199it [00:01, 1717035.91it/s] 3178172it [00:01, 1717825.80it/s] 3349955it [00:02, 1702448.03it/s] 3522998it [00:02, 1710772.69it/s] 3698558it [00:02, 1724145.87it/s] 3871533it [00:02, 1725792.03it/s] 4044131it [00:02, 1708748.15it/s] 4218069it [00:02, 1717834.56it/s] 4393086it [00:02, 1727459.01it/s] 4569002it [00:02, 1736909.50it/s] 4745056it [00:02, 1743959.91it/s] 4920894it [00:02, 1748256.40it/s] 5095828it [00:03, 1748559.03it/s] 5271153it [00:03, 1749791.60it/s] 5446141it [00:03, 1748547.67it/s] 5621002it [00:03, 1738810.42it/s] 5794899it [00:03, 1734743.49it/s] 5968385it [00:03, 1729348.26it/s] 6143444it [00:03, 1735664.39it/s] 6317021it [00:03, 1730997.93it/s] 6490662it [00:03, 1732592.26it/s] 6663928it [00:03, 1722726.42it/s] 6836215it [00:04, 1710613.89it/s] 7008321it [00:04, 1713667.90it/s] 7181013it [00:04, 1717595.87it/s] 7354903it [00:04, 1723937.16it/s] 7531240it [00:04, 1735708.00it/s] 7707088it [00:04, 1742436.19it/s] 7882379it [00:04, 1745548.76it/s] 8058477it [00:04, 1750152.02it/s] 8233976it [00:04, 1751581.45it/s] 8297353it [00:04, 1710104.03it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md.bai + merge_qc -folders aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1/aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1.md -output aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1/aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1/aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1/aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1_full_basic_stats.tsv