[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-18a6f2-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (10158345) splicing reads. 218327 splicing junctions. 171600 known splicing junctions. 46727 novel splicing junctions.
sampling 10% (20316691) splicing reads. 251872 splicing junctions. 182208 known splicing junctions. 69664 novel splicing junctions.
sampling 15% (30475036) splicing reads. 272024 splicing junctions. 187298 known splicing junctions. 84726 novel splicing junctions.
sampling 20% (40633382) splicing reads. 285476 splicing junctions. 190328 known splicing junctions. 95148 novel splicing junctions.
sampling 25% (50791728) splicing reads. 295419 splicing junctions. 192429 known splicing junctions. 102990 novel splicing junctions.
sampling 30% (60950073) splicing reads. 302989 splicing junctions. 193874 known splicing junctions. 109115 novel splicing junctions.
sampling 35% (71108419) splicing reads. 309122 splicing junctions. 195041 known splicing junctions. 114081 novel splicing junctions.
sampling 40% (81266765) splicing reads. 314011 splicing junctions. 195930 known splicing junctions. 118081 novel splicing junctions.
sampling 45% (91425110) splicing reads. 317983 splicing junctions. 196620 known splicing junctions. 121363 novel splicing junctions.
sampling 50% (101583456) splicing reads. 321277 splicing junctions. 197196 known splicing junctions. 124081 novel splicing junctions.
sampling 55% (111741802) splicing reads. 323991 splicing junctions. 197645 known splicing junctions. 126346 novel splicing junctions.
sampling 60% (121900147) splicing reads. 326297 splicing junctions. 198028 known splicing junctions. 128269 novel splicing junctions.
sampling 65% (132058493) splicing reads. 328115 splicing junctions. 198318 known splicing junctions. 129797 novel splicing junctions.
sampling 70% (142216839) splicing reads. 329704 splicing junctions. 198570 known splicing junctions. 131134 novel splicing junctions.
sampling 75% (152375184) splicing reads. 330981 splicing junctions. 198781 known splicing junctions. 132200 novel splicing junctions.
sampling 80% (162533530) splicing reads. 332016 splicing junctions. 198951 known splicing junctions. 133065 novel splicing junctions.
sampling 85% (172691876) splicing reads. 332898 splicing junctions. 199078 known splicing junctions. 133820 novel splicing junctions.
sampling 90% (182850221) splicing reads. 333523 splicing junctions. 199163 known splicing junctions. 134360 novel splicing junctions.
sampling 95% (193008567) splicing reads. 333970 splicing junctions. 199233 known splicing junctions. 134737 novel splicing junctions.
sampling 100% (203166913) splicing reads. 334220 splicing junctions. 199274 known splicing junctions. 134946 novel splicing junctions.