.command.log - Workdir - NFCORE_RNAFUSION:RNAFUSION:FUSIONINSPECTOR_WORKFLOW:FUSIONINSPECTOR (aih-tih-sc-a70f1e-R1_B23WHYVLT4_1)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2a/79320f4d364d3041cf51b47e6f81ac/.command.log
Size: 6.5 KB
Task: NFCORE_RNAFUSION:RNAFUSION:FUSIONINSPECTOR_WORKFLOW:FUSIONINSPECTOR (aih-tih-sc-a70f1e-R1_B23WHYVLT4_1)
Attempt
Content

  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ca/78884074dccfc107ef28b974127b32/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1_1.fastp.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2a/79320f4d364d3041cf51b47e6f81ac/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2a/79320f4d364d3041cf51b47e6f81ac/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ca/78884074dccfc107ef28b974127b32/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1_2.fastp.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/62/52af5fb56f6c51d2076134ce83049d/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.fusionreport.tsv
==> STAGING COMPLETE (6 inputs)

Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_pair_to_mini_genome_join.pl --fusions aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.fusionreport.tsv --gtf /tmp/nxf.vmNTrAKzF1/ctat_genome_lib_build_dir/ref_annot.gtf --genome_fa /tmp/nxf.vmNTrAKzF1/ctat_genome_lib_build_dir/ref_genome.fa --out_prefix /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1 --shrink_introns --max_intron_length 1000 
Done.
Execution Time = 0.11 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_pair_to_mini_genome_join.pl --fusions aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.fusionreport.tsv --gtf /tmp/nxf.vmNTrAKzF1/ctat_genome_lib_build_dir/ref_annot.gtf --genome_fa /tmp/nxf.vmNTrAKzF1/ctat_genome_lib_build_dir/ref_genome.fa --out_prefix /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1 --shrink_introns --max_intron_length 1000 
Running: touch /tmp/nxf.vmNTrAKzF1/chckpts_dir/fusion_contigs.ok
Running: cp /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.fa /tmp/nxf.vmNTrAKzF1/fi_workdir/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.fa
Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.fa /tmp/nxf.vmNTrAKzF1/fi_workdir/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.fa
Running: touch /tmp/nxf.vmNTrAKzF1/chckpts_dir/cp_contigs_file_workdir
Running: cp /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.gtf /tmp/nxf.vmNTrAKzF1/fi_workdir/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.gtf
Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.gtf /tmp/nxf.vmNTrAKzF1/fi_workdir/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.gtf
Running: touch /tmp/nxf.vmNTrAKzF1/chckpts_dir/cp_gtf_file_workdir.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fasta_and_gtf_to_cytoband.pl /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.fa /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.gtf > /tmp/nxf.vmNTrAKzF1/cytoBand.txt
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fasta_and_gtf_to_cytoband.pl /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.fa /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.gtf > /tmp/nxf.vmNTrAKzF1/cytoBand.txt
Running: touch /tmp/nxf.vmNTrAKzF1/chckpts_dir/cytoband.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/gtf_gene_to_bed.pl /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.gtf > /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.bed
-parsing GTF file: /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.gtf
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/gtf_gene_to_bed.pl /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.gtf > /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.bed
Running: touch /tmp/nxf.vmNTrAKzF1/chckpts_dir/merged_contig_gtf_to_bed.ok
Running: sort -k1,1 -k2,2n /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.bed > /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.bed.sorted.bed
Execution Time = 0.00 minutes. CMD: sort -k1,1 -k2,2n /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.bed > /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.bed.sorted.bed
Running: touch /tmp/nxf.vmNTrAKzF1/chckpts_dir/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.bed.bedsort.ok
Running: bgzip -f /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.bed.sorted.bed
Execution Time = 0.00 minutes. CMD: bgzip -f /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.bed.sorted.bed
Running: touch /tmp/nxf.vmNTrAKzF1/chckpts_dir/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.bed.bgzip.ok
Running: tabix -p bed /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.bed.sorted.bed.gz
Execution Time = 0.00 minutes. CMD: tabix -p bed /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.bed.sorted.bed.gz
Running: touch /tmp/nxf.vmNTrAKzF1/chckpts_dir/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.bed.tabix.ok
Running: samtools faidx /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.fa
Execution Time = 0.00 minutes. CMD: samtools faidx /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.fa
Running: touch /tmp/nxf.vmNTrAKzF1/chckpts_dir/merged_contig_fai.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/run_FI_STAR.pl --genome /tmp/nxf.vmNTrAKzF1/ctat_genome_lib_build_dir/ref_genome.fa --patch /tmp/nxf.vmNTrAKzF1/fi_workdir/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.fa --max_mate_dist 100000 -G /tmp/nxf.vmNTrAKzF1/fi_workdir/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.gtf --CPU 8 --out_prefix aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.star --out_dir /tmp/nxf.vmNTrAKzF1/fi_workdir --reads "/tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1_1.fastp.fastq.gz /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1_2.fastp.fastq.gz"
* Running CMD: /usr/local/bin/STAR  --runThreadN 8  --genomeDir /tmp/nxf.vmNTrAKzF1/ctat_genome_lib_build_dir/ref_genome.fa.star.idx  --outSAMtype BAM SortedByCoordinate  --twopassMode None  --alignSJDBoverhangMin 10  --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705  --alignInsertionFlush Right   --alignMatesGapMax 100000  --alignIntronMax 100000  --outSAMattributes All  --readFilesIn /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1_1.fastp.fastq.gz /tmp/nxf.vmNTrAKzF1/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1_2.fastp.fastq.gz  --genomeFastaFiles /tmp/nxf.vmNTrAKzF1/fi_workdir/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.fa  --outSAMfilter KeepAllAddedReferences  --sjdbGTFfile /tmp/nxf.vmNTrAKzF1/fi_workdir/aih-tih-sc-a70f1e-R1_B23WHYVLT4_1.gtf  --alignSJstitchMismatchNmax 5 -1 5 5  --scoreGapNoncan -6  --readFilesCommand 'gunzip -c' 
Jun 08 09:23:43 ..... started STAR run
Jun 08 09:23:43 ..... loading genome
Jun 08 09:25:22 ... generating Suffix Array index
Jun 08 09:29:01 ... completed Suffix Array index
Jun 08 09:29:01 ..... processing annotations GTF
Jun 08 09:29:02 ..... inserting junctions into the genome indices
Jun 08 09:30:02 ..... started mapping