+ mkdir aih-tih-sc-7c8bae-R1_B23WHYVLT4_1 + all_qc -bam aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-7c8bae-R1_B23WHYVLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai 0it [00:00, ?it/s] 139910it [00:00, 1398997.93it/s] 279810it [00:00, 1398780.23it/s] 419689it [00:00, 1390684.72it/s] 571981it [00:00, 1442475.06it/s] 731641it [00:00, 1497896.95it/s] 895072it [00:00, 1544169.32it/s] 1058371it [00:00, 1573148.93it/s] 1225234it [00:00, 1603501.32it/s] 1385600it [00:00, 1600641.96it/s] 1549475it [00:01, 1612357.33it/s] 1715663it [00:01, 1627473.35it/s] 1882451it [00:01, 1639734.73it/s] 2046432it [00:01, 1552672.79it/s] 2202649it [00:01, 1424745.34it/s] 2349798it [00:01, 1437600.66it/s] 2503955it [00:01, 1466936.54it/s] 2660783it [00:01, 1496045.63it/s] 2827488it [00:01, 1545800.88it/s] 2983037it [00:01, 1538758.58it/s] 3137586it [00:02, 1511584.88it/s] 3289280it [00:02, 1396887.69it/s] 3447049it [00:02, 1447062.09it/s] 3611976it [00:02, 1504514.47it/s] 3779907it [00:02, 1554940.31it/s] 3950387it [00:02, 1598686.54it/s] 4111263it [00:02, 1564650.38it/s] 4268550it [00:02, 1535610.83it/s] 4432138it [00:02, 1564555.58it/s] 4591354it [00:03, 1572604.37it/s] 4749219it [00:03, 1574358.30it/s] 4909833it [00:03, 1583746.67it/s] 5068418it [00:03, 1545879.53it/s] 5223328it [00:03, 1529109.04it/s] 5383073it [00:03, 1548991.34it/s] 5545551it [00:03, 1571244.46it/s] 5712954it [00:03, 1601684.47it/s] 5881182it [00:03, 1625634.56it/s] 6047350it [00:03, 1636194.29it/s] 6214136it [00:04, 1645508.50it/s] 6387227it [00:04, 1671031.93it/s] 6554391it [00:04, 1667352.83it/s] 6722775it [00:04, 1671552.21it/s] 6890687it [00:04, 1673793.47it/s] 7059860it [00:04, 1679145.07it/s] 7227792it [00:04, 1607943.91it/s] 7389233it [00:04, 1491829.29it/s] 7540316it [00:04, 1496040.62it/s] 7698764it [00:04, 1521142.07it/s] 7861570it [00:05, 1551983.69it/s] 8021804it [00:05, 1566667.55it/s] 8179133it [00:05, 1554085.76it/s] 8335009it [00:05, 1534713.18it/s] 8502422it [00:05, 1575547.55it/s] 8670512it [00:05, 1606614.61it/s] 8831464it [00:05, 1583139.83it/s] 8990327it [00:05, 1584730.95it/s] 9148984it [00:05, 1574095.34it/s] 9307624it [00:05, 1577653.64it/s] 9466951it [00:06, 1582263.01it/s] 9625251it [00:06, 1577472.75it/s] 9796195it [00:06, 1616758.62it/s] 9966302it [00:06, 1641899.75it/s] 10133844it [00:06, 1651898.41it/s] 10299902it [00:06, 1654474.46it/s] 10465381it [00:06, 1636287.61it/s] 10632166it [00:06, 1645601.78it/s] 10800309it [00:06, 1656259.11it/s] 10968433it [00:06, 1663695.62it/s] 11134837it [00:07, 1604467.15it/s] 11295748it [00:07, 1518228.40it/s] 11448690it [00:07, 1492073.38it/s] 11602048it [00:07, 1503713.84it/s] 11765243it [00:07, 1540565.88it/s] 11928329it [00:07, 1566031.96it/s] 12085579it [00:07, 1567901.43it/s] 12252873it [00:07, 1598944.56it/s] 12419913it [00:07, 1620050.71it/s] 12591434it [00:08, 1648377.29it/s] 12762684it [00:08, 1667496.94it/s] 12929561it [00:08, 1665485.89it/s] 13098526it [00:08, 1672631.02it/s] 13270086it [00:08, 1685466.94it/s] 13439462it [00:08, 1687925.78it/s] 13614917it [00:08, 1707865.05it/s] 13794474it [00:08, 1734129.79it/s] 13967908it [00:08, 1723043.02it/s] 14140243it [00:08, 1678474.43it/s] 14308347it [00:09, 1625005.75it/s] 14471303it [00:09, 1616531.58it/s] 14636684it [00:09, 1627298.95it/s] 14799657it [00:09, 1622834.69it/s] 14966403it [00:09, 1635964.44it/s] 15130138it [00:09, 1625990.37it/s] 15292837it [00:09, 1616697.89it/s] 15457684it [00:09, 1626084.21it/s] 15620355it [00:09, 1616107.07it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai 15787524it [00:09, 1632564.91it/s] 15950833it [00:10, 1606065.50it/s] 16111555it [00:10, 1587764.13it/s] 16270431it [00:10, 1559823.47it/s] 16431596it [00:10, 1574786.04it/s] 16590874it [00:10, 1580063.49it/s] 16752129it [00:10, 1589567.85it/s] 16911170it [00:10, 1576504.10it/s] 17068893it [00:10, 1574678.49it/s] 17226410it [00:10, 1545423.49it/s] 17381083it [00:10, 1535313.67it/s] 17534702it [00:11, 1514358.66it/s] 17686228it [00:11, 1513592.90it/s] 17837649it [00:11, 1496890.27it/s] 17987397it [00:11, 1478746.12it/s] 18151720it [00:11, 1526964.23it/s] 18312521it [00:11, 1550876.94it/s] 18479163it [00:11, 1585135.84it/s] 18646508it [00:11, 1611404.62it/s] 18814596it [00:11, 1632113.28it/s] 18933832it [00:11, 1582655.00it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md.bai + merge_qc -folders aih-tih-sc-7c8bae-R1_B23WHYVLT4_1/aih-tih-sc-7c8bae-R1_B23WHYVLT4_1.md -output aih-tih-sc-7c8bae-R1_B23WHYVLT4_1/aih-tih-sc-7c8bae-R1_B23WHYVLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-7c8bae-R1_B23WHYVLT4_1/aih-tih-sc-7c8bae-R1_B23WHYVLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-7c8bae-R1_B23WHYVLT4_1/aih-tih-sc-7c8bae-R1_B23WHYVLT4_1_full_basic_stats.tsv