[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-027d27-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	254029602
Known Splicing Events:	250976017
Partial Novel Splicing Events:	1649149
Novel Splicing Events:	1321407
Filtered Splicing Events:	83029

Total splicing  Junctions:	504055
Known Splicing Junctions:	272501
Partial Novel Splicing Junctions:	144576
Novel Splicing Junctions:	86978

===================================================================
Create BED file ...
Create Interact file ...