Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/af/ff6f471a2567bd706601567e3178a2/aih-tih-sc-027d27-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/af/9bd7f6623e3cc7c6fd351e821160d7/aih-tih-sc-027d27-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/bb/013786bf60e48795a82bf865049234/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/eb/9280d051a76962c8beb06959d2202b/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/eb/9280d051a76962c8beb06959d2202b/.command.run
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-027d27-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (12696825) splicing reads. 259839 splicing junctions. 188523 known splicing junctions. 71316 novel splicing junctions.
sampling 10% (25393650) splicing reads. 312983 splicing junctions. 201085 known splicing junctions. 111898 novel splicing junctions.
sampling 15% (38090475) splicing reads. 348798 splicing junctions. 207701 known splicing junctions. 141097 novel splicing junctions.
sampling 20% (50787300) splicing reads. 376003 splicing junctions. 211988 known splicing junctions. 164015 novel splicing junctions.
sampling 25% (63484125) splicing reads. 397561 splicing junctions. 214969 known splicing junctions. 182592 novel splicing junctions.
sampling 30% (76180950) splicing reads. 415077 splicing junctions. 217158 known splicing junctions. 197919 novel splicing junctions.
sampling 35% (88877775) splicing reads. 429735 splicing junctions. 218990 known splicing junctions. 210745 novel splicing junctions.
sampling 40% (101574600) splicing reads. 442174 splicing junctions. 220469 known splicing junctions. 221705 novel splicing junctions.
sampling 45% (114271425) splicing reads. 452806 splicing junctions. 221703 known splicing junctions. 231103 novel splicing junctions.
sampling 50% (126968250) splicing reads. 461988 splicing junctions. 222725 known splicing junctions. 239263 novel splicing junctions.
sampling 55% (139665075) splicing reads. 469879 splicing junctions. 223569 known splicing junctions. 246310 novel splicing junctions.
sampling 60% (152361900) splicing reads. 476711 splicing junctions. 224319 known splicing junctions. 252392 novel splicing junctions.
sampling 65% (165058725) splicing reads. 482417 splicing junctions. 224889 known splicing junctions. 257528 novel splicing junctions.
sampling 70% (177755550) splicing reads. 487383 splicing junctions. 225377 known splicing junctions. 262006 novel splicing junctions.
sampling 75% (190452375) splicing reads. 491719 splicing junctions. 225786 known splicing junctions. 265933 novel splicing junctions.
sampling 80% (203149200) splicing reads. 495262 splicing junctions. 226164 known splicing junctions. 269098 novel splicing junctions.
sampling 85% (215846025) splicing reads. 498230 splicing junctions. 226439 known splicing junctions. 271791 novel splicing junctions.
sampling 90% (228542850) splicing reads. 500618 splicing junctions. 226670 known splicing junctions. 273948 novel splicing junctions.
sampling 95% (241239675) splicing reads. 502525 splicing junctions. 226838 known splicing junctions. 275687 novel splicing junctions.
sampling 100% (253936500) splicing reads. 503860 splicing junctions. 226983 known splicing junctions. 276877 novel splicing junctions.
null device 
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