[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-37dd87-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (16800451) splicing reads. 266733 splicing junctions. 186651 known splicing junctions. 80082 novel splicing junctions.
sampling 10% (33600903) splicing reads. 327814 splicing junctions. 199857 known splicing junctions. 127957 novel splicing junctions.
sampling 15% (50401354) splicing reads. 371746 splicing junctions. 207050 known splicing junctions. 164696 novel splicing junctions.
sampling 20% (67201806) splicing reads. 406076 splicing junctions. 211830 known splicing junctions. 194246 novel splicing junctions.
sampling 25% (84002257) splicing reads. 434639 splicing junctions. 215314 known splicing junctions. 219325 novel splicing junctions.
sampling 30% (100802709) splicing reads. 458753 splicing junctions. 218022 known splicing junctions. 240731 novel splicing junctions.
sampling 35% (117603160) splicing reads. 479270 splicing junctions. 220082 known splicing junctions. 259188 novel splicing junctions.
sampling 40% (134403612) splicing reads. 497427 splicing junctions. 221803 known splicing junctions. 275624 novel splicing junctions.
sampling 45% (151204063) splicing reads. 513054 splicing junctions. 223251 known splicing junctions. 289803 novel splicing junctions.
sampling 50% (168004515) splicing reads. 527297 splicing junctions. 224457 known splicing junctions. 302840 novel splicing junctions.
sampling 55% (184804967) splicing reads. 539537 splicing junctions. 225497 known splicing junctions. 314040 novel splicing junctions.
sampling 60% (201605418) splicing reads. 550486 splicing junctions. 226382 known splicing junctions. 324104 novel splicing junctions.
sampling 65% (218405870) splicing reads. 560237 splicing junctions. 227214 known splicing junctions. 333023 novel splicing junctions.
sampling 70% (235206321) splicing reads. 568841 splicing junctions. 227861 known splicing junctions. 340980 novel splicing junctions.
sampling 75% (252006773) splicing reads. 576307 splicing junctions. 228423 known splicing junctions. 347884 novel splicing junctions.
sampling 80% (268807224) splicing reads. 582663 splicing junctions. 228879 known splicing junctions. 353784 novel splicing junctions.
sampling 85% (285607676) splicing reads. 588378 splicing junctions. 229282 known splicing junctions. 359096 novel splicing junctions.
sampling 90% (302408127) splicing reads. 593113 splicing junctions. 229632 known splicing junctions. 363481 novel splicing junctions.
sampling 95% (319208579) splicing reads. 596991 splicing junctions. 229903 known splicing junctions. 367088 novel splicing junctions.
sampling 100% (336009031) splicing reads. 600129 splicing junctions. 230125 known splicing junctions. 370004 novel splicing junctions.