[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b3c1e-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (14626031) splicing reads. 263207 splicing junctions. 188604 known splicing junctions. 74603 novel splicing junctions.
sampling 10% (29252063) splicing reads. 314670 splicing junctions. 200362 known splicing junctions. 114308 novel splicing junctions.
sampling 15% (43878095) splicing reads. 348741 splicing junctions. 206541 known splicing junctions. 142200 novel splicing junctions.
sampling 20% (58504127) splicing reads. 373396 splicing junctions. 210562 known splicing junctions. 162834 novel splicing junctions.
sampling 25% (73130158) splicing reads. 392373 splicing junctions. 213202 known splicing junctions. 179171 novel splicing junctions.
sampling 30% (87756190) splicing reads. 407506 splicing junctions. 215113 known splicing junctions. 192393 novel splicing junctions.
sampling 35% (102382222) splicing reads. 419917 splicing junctions. 216652 known splicing junctions. 203265 novel splicing junctions.
sampling 40% (117008254) splicing reads. 430078 splicing junctions. 217874 known splicing junctions. 212204 novel splicing junctions.
sampling 45% (131634285) splicing reads. 438738 splicing junctions. 218886 known splicing junctions. 219852 novel splicing junctions.
sampling 50% (146260317) splicing reads. 446184 splicing junctions. 219679 known splicing junctions. 226505 novel splicing junctions.
sampling 55% (160886349) splicing reads. 452401 splicing junctions. 220333 known splicing junctions. 232068 novel splicing junctions.
sampling 60% (175512381) splicing reads. 457635 splicing junctions. 220905 known splicing junctions. 236730 novel splicing junctions.
sampling 65% (190138412) splicing reads. 462136 splicing junctions. 221360 known splicing junctions. 240776 novel splicing junctions.
sampling 70% (204764444) splicing reads. 465940 splicing junctions. 221758 known splicing junctions. 244182 novel splicing junctions.
sampling 75% (219390476) splicing reads. 469187 splicing junctions. 222086 known splicing junctions. 247101 novel splicing junctions.
sampling 80% (234016508) splicing reads. 471912 splicing junctions. 222347 known splicing junctions. 249565 novel splicing junctions.
sampling 85% (248642539) splicing reads. 474133 splicing junctions. 222589 known splicing junctions. 251544 novel splicing junctions.
sampling 90% (263268571) splicing reads. 475887 splicing junctions. 222768 known splicing junctions. 253119 novel splicing junctions.
sampling 95% (277894603) splicing reads. 477208 splicing junctions. 222905 known splicing junctions. 254303 novel splicing junctions.
sampling 100% (292520635) splicing reads. 478217 splicing junctions. 223012 known splicing junctions. 255205 novel splicing junctions.