[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-20b7e4-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	89540130
Known Splicing Events:	88600292
Partial Novel Splicing Events:	534157
Novel Splicing Events:	360813
Filtered Splicing Events:	44868

Total splicing  Junctions:	253075
Known Splicing Junctions:	193364
Partial Novel Splicing Junctions:	36138
Novel Splicing Junctions:	23573

===================================================================
Create BED file ...
Create Interact file ...