+ mkdir aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1 + all_qc -bam aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai 155852it [00:00, 1558428.60it/s] 318887it [00:00, 1600663.35it/s] 483401it [00:00, 1620935.21it/s] 645495it [00:00, 1611281.75it/s] 815174it [00:00, 1641950.99it/s] 983277it [00:00, 1655121.48it/s] 1148804it [00:00, 1628546.65it/s] 1319862it [00:00, 1654322.68it/s] 1493307it [00:00, 1679133.62it/s] 1661306it [00:01, 1666172.29it/s] 1827998it [00:01, 1648338.19it/s] 1995048it [00:01, 1654959.99it/s] 2163540it [00:01, 1663922.34it/s] 2336600it [00:01, 1683830.78it/s] 2507902it [00:01, 1692564.74it/s] 2680130it [00:01, 1701456.75it/s] 2850307it [00:01, 1697146.42it/s] 3020405it [00:01, 1698092.36it/s] 3198833it [00:01, 1723789.78it/s] 3371230it [00:02, 1709794.97it/s] 3542246it [00:02, 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1419648.49it/s] 8472802it [00:05, 1427050.46it/s] 8615723it [00:05, 1404171.35it/s] 8756342it [00:05, 1400606.87it/s] 8907757it [00:05, 1434019.80it/s] 9056284it [00:05, 1449154.96it/s] 9205867it [00:05, 1462925.15it/s] 9352262it [00:05, 1445541.08it/s] 9511598it [00:05, 1489277.85it/s] 9671059it [00:06, 1520556.78it/s] 9834632it [00:06, 1554781.76it/s] 9990218it [00:06, 1539173.05it/s] 10144240it [00:06, 1515663.75it/s] 10312244it [00:06, 1564059.74it/s] 10468832it [00:06, 1562478.72it/s] 10625571it [00:06, 1563910.26it/s] 10782052it [00:06, 1518107.59it/s] 10934193it [00:06, 1438247.96it/s] 11078974it [00:06, 1421743.89it/s] 11221778it [00:07, 1375446.65it/s] 11363505it [00:07, 1387236.69it/s] 11502710it [00:07, 1379307.20it/s] 11640957it [00:07, 1367464.96it/s] 11777911it [00:07, 1366039.64it/s] 11914874it [00:07, 1367063.28it/s] 12067015it [00:07, 1412541.76it/s] 12216684it [00:07, 1437427.63it/s] 12364532it [00:07, 1449608.35it/s] 12512519it [00:07, 1458601.47it/s] 12664931it [00:08, 1478120.41it/s] 12812803it [00:08, 1477901.74it/s] 12963165it [00:08, 1485569.39it/s] 13111753it [00:08, 1479389.08it/s] 13268532it [00:08, 1505776.52it/s] 13422487it [00:08, 1515851.18it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai 13574408it [00:08, 1516823.69it/s] 13726107it [00:08, 1497043.48it/s] 13875879it [00:08, 1493529.12it/s] 14028718it [00:08, 1503866.24it/s] 14181296it [00:09, 1510374.01it/s] 14332367it [00:09, 1493087.41it/s] 14482137it [00:09, 1494417.08it/s] 14631623it [00:09, 1373813.59it/s] 14770887it [00:09, 1314936.93it/s] 14916650it [00:09, 1354397.95it/s] 15060729it [00:09, 1378717.22it/s] 15203537it [00:09, 1392897.29it/s] 15345025it [00:09, 1399248.42it/s] 15485710it [00:10, 1401446.79it/s] 15636737it [00:10, 1433602.89it/s] 15780452it [00:10, 1420135.62it/s] 15922732it [00:10, 1418889.78it/s] 16071691it [00:10, 1439831.94it/s] 16215827it [00:10, 1426017.36it/s] 16364215it [00:10, 1443125.65it/s] 16508639it [00:10, 1379342.24it/s] 16650679it [00:10, 1391130.45it/s] 16795230it [00:10, 1406987.91it/s] 16949944it [00:11, 1448102.18it/s] 17104541it [00:11, 1477048.33it/s] 17262406it [00:11, 1507220.09it/s] 17413354it [00:11, 1485699.46it/s] 17564564it [00:11, 1493459.73it/s] 17672038it [00:11, 1531509.36it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md.bai + merge_qc -folders aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1/aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1.md -output aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1/aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1/aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1/aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1_full_basic_stats.tsv