[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-099ac4-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (15780231) splicing reads. 269008 splicing junctions. 185877 known splicing junctions. 83131 novel splicing junctions.
sampling 10% (31560462) splicing reads. 328284 splicing junctions. 198806 known splicing junctions. 129478 novel splicing junctions.
sampling 15% (47340693) splicing reads. 369705 splicing junctions. 205604 known splicing junctions. 164101 novel splicing junctions.
sampling 20% (63120924) splicing reads. 401452 splicing junctions. 210230 known splicing junctions. 191222 novel splicing junctions.
sampling 25% (78901155) splicing reads. 427080 splicing junctions. 213530 known splicing junctions. 213550 novel splicing junctions.
sampling 30% (94681386) splicing reads. 448905 splicing junctions. 216047 known splicing junctions. 232858 novel splicing junctions.
sampling 35% (110461618) splicing reads. 467418 splicing junctions. 218004 known splicing junctions. 249414 novel splicing junctions.
sampling 40% (126241849) splicing reads. 483054 splicing junctions. 219647 known splicing junctions. 263407 novel splicing junctions.
sampling 45% (142022080) splicing reads. 496905 splicing junctions. 221041 known splicing junctions. 275864 novel splicing junctions.
sampling 50% (157802311) splicing reads. 508890 splicing junctions. 222211 known splicing junctions. 286679 novel splicing junctions.
sampling 55% (173582542) splicing reads. 519269 splicing junctions. 223174 known splicing junctions. 296095 novel splicing junctions.
sampling 60% (189362773) splicing reads. 528579 splicing junctions. 224028 known splicing junctions. 304551 novel splicing junctions.
sampling 65% (205143004) splicing reads. 536686 splicing junctions. 224716 known splicing junctions. 311970 novel splicing junctions.
sampling 70% (220923236) splicing reads. 543717 splicing junctions. 225312 known splicing junctions. 318405 novel splicing junctions.
sampling 75% (236703467) splicing reads. 550037 splicing junctions. 225850 known splicing junctions. 324187 novel splicing junctions.
sampling 80% (252483698) splicing reads. 555330 splicing junctions. 226295 known splicing junctions. 329035 novel splicing junctions.
sampling 85% (268263929) splicing reads. 559863 splicing junctions. 226666 known splicing junctions. 333197 novel splicing junctions.
sampling 90% (284044160) splicing reads. 563587 splicing junctions. 226998 known splicing junctions. 336589 novel splicing junctions.
sampling 95% (299824391) splicing reads. 566615 splicing junctions. 227229 known splicing junctions. 339386 novel splicing junctions.
sampling 100% (315604623) splicing reads. 569000 splicing junctions. 227408 known splicing junctions. 341592 novel splicing junctions.