Done.
-parsing GTF file: /tmp/nxf.o3F6AwNrIQ/aih-tih-sc-505123-R1_B23WHYVLT4_1.gtf
* Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.o3F6AwNrIQ/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.o3F6AwNrIQ/aih-tih-sc-505123-R1_B23WHYVLT4_1_1.fastp.fastq.gz /tmp/nxf.o3F6AwNrIQ/aih-tih-sc-505123-R1_B23WHYVLT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.o3F6AwNrIQ/fi_workdir/aih-tih-sc-505123-R1_B23WHYVLT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.o3F6AwNrIQ/fi_workdir/aih-tih-sc-505123-R1_B23WHYVLT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c'
* Running CMD: mv Aligned.sortedByCoord.out.bam aih-tih-sc-505123-R1_B23WHYVLT4_1.star.sortedByCoord.out.bam
* Running CMD: samtools index aih-tih-sc-505123-R1_B23WHYVLT4_1.star.sortedByCoord.out.bam
09:02:24 : INFO : Done.
-parsing GTF file: /tmp/nxf.o3F6AwNrIQ/fi_workdir/aih-tih-sc-505123-R1_B23WHYVLT4_1.gtf
-parsing /tmp/nxf.o3F6AwNrIQ/fi_workdir/aih-tih-sc-505123-R1_B23WHYVLT4_1.star.cSorted.dupsMarked.bam
[10000]
[20000]
[30000]
[40000]
[50000]
[60000]
[70000]
[80000]
[90000]
[100000]
[110000]
[120000]
[130000]
[140000]
[150000]
[160000]
[170000]
[180000]
[190000]
[200000]
[210000]
[220000]
[230000]
[240000]
[250000]
[260000]
[270000]
[280000]
[290000]
[300000]
[310000]
[320000]
[330000]
[340000]
[350000]
[360000]
[370000]
[380000]
[390000]
[400000]
[410000]
[420000]
[430000]
[440000]
[450000]
[460000]
[470000]
[480000]
[490000]
[500000]
[510000]
[520000]
[530000]
[540000]
[550000]
[560000]
[570000]
[580000]
[590000]
[600000]
[610000] -done parsing /tmp/nxf.o3F6AwNrIQ/fi_workdir/aih-tih-sc-505123-R1_B23WHYVLT4_1.star.cSorted.dupsMarked.bam. Extracting junction info.
junction read elimination tally: $VAR1 = {
'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 2,
'num_hits: 4 != num_counted_on_fusion_contigs 1 ' => 22,
'per_id < 96' => 2368,
'num genes matched < 2' => 4,
'num_hits: 4 != num_counted_on_fusion_contigs 3 ' => 30,
'num_hits: 5 != num_counted_on_fusion_contigs 3 ' => 12,
'small anchor length' => 44,
'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 23,
'excessive soft clipping' => 2372,
' ** passed ** ' => 8751,
'low complexity anchor region' => 3,
'seq-similar region overlap' => 1
};
-writing fusion junction support info.
-outputting the spanning read info: /tmp/nxf.o3F6AwNrIQ/fi_workdir/aih-tih-sc-505123-R1_B23WHYVLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info.
- counting read alignments among fusion contigs.
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000]
[9000]
[10000]
[11000]
[12000]
[13000]
[14000]
[15000]
[16000]
[17000]
[18000]
[19000]
[20000]
[21000]
[22000]
[23000]
[24000]
[25000]
[26000]
[27000]
[28000]
[29000]
[30000]
[31000]
[32000]
[33000]
[34000]
[35000]
[36000]
[37000]
[38000]
[39000]
[40000]
[41000]
[42000]
[43000]
[44000]
[45000]
[46000] -fusion SPANNING read extraction for scaff: PCDHA3--PCDHA13
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000]
[9000]
[10000]
[11000]
[12000]
[13000]
[14000]
[15000]
[16000]
[17000]
[18000]
[19000]
[20000]
[21000]
[22000]
[23000]
[24000]
[25000]
[26000]
[27000]
[28000]
[29000]
[30000]
[31000]
[32000]
[33000]
[34000]
[35000]
[36000]
[37000]
[38000]
[39000]
[40000]
[41000]
[42000]
[43000]
[44000] -fusion SPANNING read extraction for scaff: PCDHA3--PCDHAC1
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000]
[9000]
[10000]
[11000]
[12000]
[13000]
[14000]
[15000]
[16000]
[17000]
[18000]
[19000]
[20000]
[21000]
[22000]
[23000]
[24000]
[25000]
[26000]
[27000]
[28000]
[29000]
[30000]
[31000]
[32000]
[33000]
[34000]
[35000]
[36000]
[37000]
[38000] -fusion SPANNING read extraction for scaff: PCDHA3--PCDHAC2
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000]
[9000]
[10000]
[11000]
[12000]
[13000]
[14000]
[15000]
[16000]
[17000]
[18000]
[19000]
[20000]
[21000]
[22000]
[23000]
[24000]
[25000]
[26000]
[27000]
[28000]
[29000]
[30000] -fusion SPANNING read extraction for scaff: PCDHA4--PCDHAC2
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000]
[9000]
[10000]
[11000]
[12000]
[13000]
[14000]
[15000]
[16000]
[17000]
[18000]
[19000]
[20000]
[21000]
[22000]
[23000]
[24000]
[25000]
[26000]
[27000]
[28000]
[29000]
[30000]
[31000]
[32000]
[33000]
[34000]
[35000]
[36000] -fusion SPANNING read extraction for scaff: PCDHA4--PCDHAC1
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000]
[9000]
[10000]
[11000]
[12000]
[13000]
[14000]
[15000]
[16000]
[17000]
[18000]
[19000]
[20000]
[21000]
[22000]
[23000]
[24000]
[25000]
[26000]
[27000]
[28000]
[29000]
[30000]
[31000]
[32000]
[33000]
[34000]
[35000]
[36000]
[37000]
[38000] -fusion SPANNING read extraction for scaff: PCDHA4--PCDHA13
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000]
[9000]
[10000]
[11000]
[12000]
[13000]
[14000]
[15000]
[16000]
[17000]
[18000]
[19000]
[20000]
[21000]
[22000]
[23000]
[24000]
[25000]
[26000]
[27000]
[28000]
[29000]
[30000]
[31000]
[32000]
[33000]
[34000]
[35000]
[36000]
[37000]
[38000]
[39000]
[40000]
[41000] -fusion SPANNING read extraction for scaff: PCDHA6--PCDHAC2
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000]
[9000]
[10000]
[11000]
[12000]
[13000]
[14000]
[15000]
[16000]
[17000]
[18000]
[19000]
[20000]
[21000]
[22000]
[23000]
[24000]
[25000]
[26000]
[27000]
[28000]
[29000]
[30000]
[31000]
[32000]
[33000]
[34000]
[35000]
[36000]
[37000]
[38000]
[39000]
[40000]
[41000]
[42000]
[43000]
[44000]
[45000]
[46000]
[47000] -fusion SPANNING read extraction for scaff: PCDHA6--PCDHAC1
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000]
[9000]
[10000]
[11000]
[12000]
[13000]
[14000]
[15000]
[16000]
[17000]
[18000]
[19000]
[20000]
[21000]
[22000]
[23000]
[24000]
[25000]
[26000]
[27000]
[28000]
[29000]
[30000]
[31000]
[32000]
[33000]
[34000]
[35000]
[36000]
[37000]
[38000]
[39000]
[40000]
[41000]
[42000]
[43000]
[44000]
[45000]
[46000]
[47000]
[48000] -fusion SPANNING read extraction for scaff: PCDHA6--PCDHA13
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000]
[9000]
[10000]
[11000]
[12000]
[13000]
[14000]
[15000]
[16000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGC3
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGC5
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000]
[9000]
[10000]
[11000]
[12000]
[13000]
[14000]
[15000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGC4
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000]
[9000]
[10000]
[11000]
[12000]
[13000]
[14000]
[15000]
[16000]
[17000]
[18000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA8
[1000]
[2000]
[3000]
[4000]
[5000]
[6000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGB6
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA10
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000]
[9000]
[10000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGB7
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000]
[9000]
[10000]
[11000]
[12000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA11
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000]
[9000]
[10000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA9
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA12
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000]
[9000]
[10000]
[11000]
[12000]
[13000]
[14000]
[15000]
[16000]
[17000] -fusion SPANNING read extraction for scaff: PTPN20--BMS1P7
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000]
[9000]
[10000]
[11000]
[12000]
[13000]
[14000]
[15000]
[16000]
[17000]
[18000]
[19000]
[20000]
[21000]
[22000]
[23000]
[24000]
[25000]
[26000]
[27000]
[28000]
[29000]
[30000]
[31000]
[32000]
[33000]
[34000]
[35000]
[36000]
[37000]
[38000]
[39000]
[40000]
[41000]
[42000]
[43000]
[44000]
[45000]
[46000]
[47000]
[48000]
[49000]
[50000]
[51000]
[52000]
[53000]
[54000]
[55000]
[56000]
[57000]
[58000]
[59000]
[60000]
[61000]
[62000]
[63000]
[64000]
[65000]
[66000]
[67000]
[68000]
[69000]
[70000]
[71000]
[72000] -fusion SPANNING read extraction for scaff: RASA4--POLR2J2
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000]
[8000]
[9000]
[10000]
[11000]
[12000]
[13000]
[14000]
[15000]
[16000]
[17000]
[18000]
[19000]
[20000]
[21000]
[22000]
[23000]
[24000]
[25000]
[26000]
[27000] -fusion SPANNING read extraction for scaff: TLK2--AC240565.1
[1000]
[2000]
[3000]
[4000]
[5000]
[6000]
[7000] -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1
-filtered reads reasons: $VAR1 = {
'seq similar region alignment' => 10422,
'lacks exon overlap' => 1901
};
EM: Starting log likelihood: -5426.337573
EM: Round [1] log likelihood: -5426.157316
EM: Round [2] log likelihood: -5426.152535
EM: Round [3] log likelihood: -5426.152345
EM: Round [4] log likelihood: -5426.152337
EM: Stopping iterations at round 4 due to insufficient improvement in likelihood.
[bam_sort_core] merging from 0 files and 8 in-memory blocks...
-extracting read coordinates from /tmp/nxf.o3F6AwNrIQ/aih-tih-sc-505123-R1_B23WHYVLT4_1.consolidated.bam into /tmp/nxf.o3F6AwNrIQ/aih-tih-sc-505123-R1_B23WHYVLT4_1.consolidated.bam.read_coords
CMD: touch /tmp/nxf.o3F6AwNrIQ/aih-tih-sc-505123-R1_B23WHYVLT4_1.consolidated.bam.read_coords.ok
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.o3F6AwNrIQ/aih-tih-sc-505123-R1_B23WHYVLT4_1.consolidated.bam.read_coords > /tmp/nxf.o3F6AwNrIQ/aih-tih-sc-505123-R1_B23WHYVLT4_1.consolidated.bam.read_coords.sort_by_readname
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.o3F6AwNrIQ/aih-tih-sc-505123-R1_B23WHYVLT4_1.consolidated.bam.frag_coords > /tmp/nxf.o3F6AwNrIQ/aih-tih-sc-505123-R1_B23WHYVLT4_1.consolidated.bam.frag_coords.coord_sorted
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.o3F6AwNrIQ/fi_workdir/aih-tih-sc-505123-R1_B23WHYVLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.o3F6AwNrIQ/fi_workdir/aih-tih-sc-505123-R1_B23WHYVLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50
CMD: cp /tmp/nxf.o3F6AwNrIQ/fi_workdir/aih-tih-sc-505123-R1_B23WHYVLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.o3F6AwNrIQ/aih-tih-sc-505123-R1_B23WHYVLT4_1.post_blast_and_promiscuity_filter
-done, see /tmp/nxf.o3F6AwNrIQ/aih-tih-sc-505123-R1_B23WHYVLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass
EM: Starting log likelihood: -3391.707586
EM: Round [1] log likelihood: -3391.590994
EM: Round [2] log likelihood: -3391.586262
EM: Round [3] log likelihood: -3391.586072
EM: Round [4] log likelihood: -3391.586064
EM: Stopping iterations at round 4 due to insufficient improvement in likelihood.
-total frags in /tmp/nxf.o3F6AwNrIQ/aih-tih-sc-505123-R1_B23WHYVLT4_1_1.fastp.fastq.gz: 133409442
Warning - not locating file: /tmp/nxf.o3F6AwNrIQ/aih-tih-sc-505123-R1_B23WHYVLT4_1.gmap_trinity_GG.fusions.gff3.bed
WARNING, no entry stored in dbm for [ENSG00000270025.2]
WARNING, no entry stored in dbm for [ENSG00000270025.2]
WARNING, no entry stored in dbm for [ENSG00000270025.2]
WARNING, no entry stored in dbm for [ENSG00000270025.2]
WARNING, no entry stored in dbm for [ENSG00000270025.2]
WARNING, no entry stored in dbm for [ENSG00000270025.2]
WARNING, no entry stored in dbm for [ENSG00000226049.3]
WARNING, no entry stored in dbm for [ENSG00000260404.3]
WARNING, no entry stored in dbm for [ENSG00000280136.2]
ls: cannot access 'IGV_inputs': No such file or directory