+ mkdir aih-tih-sc-5c902f-R1_B23WHYVLT4_1 + all_qc -bam aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-5c902f-R1_B23WHYVLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai 90694it [00:00, 906800.34it/s] 181375it [00:00, 871660.26it/s] 271549it [00:00, 885065.85it/s] 360136it [00:00, 776797.66it/s] 449758it [00:00, 815958.89it/s] 532811it [00:00, 783153.95it/s] 644791it [00:00, 885145.85it/s] 749475it [00:00, 933983.33it/s] 844163it [00:00, 930224.58it/s] 938065it [00:01, 826498.69it/s] 1036808it [00:01, 870701.43it/s] 1126142it [00:01, 866085.60it/s] 1217800it [00:01, 880434.01it/s] 1308765it [00:01, 888838.54it/s] 1405706it [00:01, 912360.79it/s] 1497617it [00:01, 885294.31it/s] 1588816it [00:01, 893002.69it/s] 1684597it [00:01, 911940.67it/s] 1792033it [00:02, 959730.30it/s] 1903116it [00:02, 1004468.12it/s] 2016997it [00:02, 1044395.66it/s] 2129759it [00:02, 1069188.09it/s] 2241863it [00:02, 1084657.98it/s] 2354008it [00:02, 1095644.25it/s] 2467316it [00:02, 1106827.46it/s] 2580719it [00:02, 1114910.05it/s] 2693272it [00:02, 1118070.46it/s] 2807572it [00:02, 1125522.84it/s] 2920386it [00:03, 1126286.13it/s] 3034095it [00:03, 1129508.52it/s] 3147432it [00:03, 1130557.00it/s] 3260692it [00:03, 1131100.50it/s] 3375090it [00:03, 1134942.66it/s] 3488589it [00:03, 1134455.81it/s] 3603074it [00:03, 1137442.12it/s] 3716995it [00:03, 1137952.26it/s] 3831839it [00:03, 1141068.65it/s] 3945948it [00:03, 1136123.53it/s] 4060097it [00:04, 1137708.38it/s] 4173873it [00:04, 1133783.73it/s] 4287257it [00:04, 1131026.71it/s] 4400364it [00:04, 1121067.66it/s] 4512490it [00:04, 1114915.73it/s] 4627081it [00:04, 1124077.61it/s] 4739510it [00:04, 1059901.27it/s] 4846235it [00:04, 1025601.40it/s] 4959351it [00:04, 1055464.34it/s] 5073442it [00:04, 1080050.88it/s] 5181985it [00:05, 1072329.26it/s] 5296090it [00:05, 1092389.19it/s] 5409188it [00:05, 1103724.00it/s] 5524161it [00:05, 1117324.64it/s] 5637710it [00:05, 1122639.01it/s] 5751503it [00:05, 1127112.15it/s] 5866003it [00:05, 1132433.33it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai 5979317it [00:05, 1131101.47it/s] 6092477it [00:05, 1123637.13it/s] 6204885it [00:05, 1122035.21it/s] 6317937it [00:06, 1124545.00it/s] 6430587it [00:06, 1125106.12it/s] 6544099it [00:06, 1128080.02it/s] 6658703it [00:06, 1133436.08it/s] 6773327it [00:06, 1137253.25it/s] 6888445it [00:06, 1141405.23it/s] 7003718it [00:06, 1144779.19it/s] 7118200it [00:06, 1144098.19it/s] 7232613it [00:06, 1141928.19it/s] 7348421it [00:06, 1146739.52it/s] 7463098it [00:07, 1145981.98it/s] 7577699it [00:07, 1142946.53it/s] 7691997it [00:07, 1137701.22it/s] 7805774it [00:07, 1131991.34it/s] 7918982it [00:07, 1126756.39it/s] 8032978it [00:07, 1130561.48it/s] 8146043it [00:07, 1128848.83it/s] 8258934it [00:07, 1125912.86it/s] 8371774it [00:07, 1126633.60it/s] 8484441it [00:07, 1123685.69it/s] 8597974it [00:08, 1127059.13it/s] 8711314it [00:08, 1128832.41it/s] 8824201it [00:08, 1123345.86it/s] 8938249it [00:08, 1128435.54it/s] 9052265it [00:08, 1131914.86it/s] 9165463it [00:08, 1120957.99it/s] 9277585it [00:08, 1118264.70it/s] 9389787it [00:08, 1119363.83it/s] 9504118it [00:08, 1126488.91it/s] 9618059it [00:08, 1130328.18it/s] 9732660it [00:09, 1134997.71it/s] 9846169it [00:09, 1132495.11it/s] 9959426it [00:09, 1127264.02it/s] 10073027it [00:09, 1129814.01it/s] 10089038it [00:09, 1073453.48it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md.bai + merge_qc -folders aih-tih-sc-5c902f-R1_B23WHYVLT4_1/aih-tih-sc-5c902f-R1_B23WHYVLT4_1.md -output aih-tih-sc-5c902f-R1_B23WHYVLT4_1/aih-tih-sc-5c902f-R1_B23WHYVLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-5c902f-R1_B23WHYVLT4_1/aih-tih-sc-5c902f-R1_B23WHYVLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-5c902f-R1_B23WHYVLT4_1/aih-tih-sc-5c902f-R1_B23WHYVLT4_1_full_basic_stats.tsv