+ mkdir aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1 + all_qc -bam aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai 153568it [00:00, 1535337.37it/s] 307102it [00:00, 1485705.61it/s] 455782it [00:00, 1462251.28it/s] 612105it [00:00, 1501257.02it/s] 774796it [00:00, 1545930.73it/s] 939487it [00:00, 1579923.49it/s] 1097601it [00:00, 1544572.72it/s] 1252278it [00:00, 1483983.73it/s] 1418265it [00:00, 1536677.39it/s] 1576241it [00:01, 1549560.33it/s] 1731640it [00:01, 1515827.75it/s] 1903192it [00:01, 1574721.25it/s] 2061131it [00:01, 1564017.40it/s] 2231146it [00:01, 1604269.21it/s] 2400573it [00:01, 1630838.04it/s] 2563909it [00:01, 1406283.83it/s] 2710055it [00:01, 1173568.31it/s] 2867568it [00:01, 1270086.11it/s] 3021776it [00:02, 1339606.14it/s] 3190048it [00:02, 1431382.66it/s] 3353600it [00:02, 1487805.05it/s] 3522978it [00:02, 1546105.33it/s] 3695476it [00:02, 1597582.50it/s] 3868009it [00:02, 1634787.61it/s] 4035000it [00:02, 1645071.27it/s] 4206322it [00:02, 1665078.76it/s] 4373916it [00:02, 1650264.55it/s] 4539725it [00:02, 1576219.08it/s] 4698530it [00:03, 1471267.34it/s] 4847611it [00:03, 1403635.72it/s] 4989607it [00:03, 1335096.93it/s] 5126169it [00:03, 1343301.21it/s] 5261555it [00:03, 1341096.95it/s] 5403934it [00:03, 1364433.82it/s] 5545613it [00:03, 1379510.22it/s] 5684074it [00:03, 1271391.03it/s] 5850333it [00:03, 1379794.12it/s] 6019690it [00:04, 1468796.13it/s] 6185915it [00:04, 1524557.45it/s] 6352514it [00:04, 1565744.60it/s] 6522127it [00:04, 1603937.81it/s] 6683484it [00:04, 1548999.55it/s] 6839421it [00:04, 1520246.93it/s] 7002269it [00:04, 1551287.54it/s] 7161973it [00:04, 1564590.92it/s] 7318932it [00:04, 1564043.60it/s] 7483978it [00:05, 1589554.66it/s] 7643216it [00:05, 1574966.12it/s] 7811420it [00:05, 1606616.84it/s] 7981300it [00:05, 1633943.89it/s] 8146715it [00:05, 1639865.06it/s] 8312928it [00:05, 1646484.04it/s] 8479601it [00:05, 1652505.49it/s] 8647642it [00:05, 1660817.81it/s] 8816720it [00:05, 1669756.88it/s] 8983730it [00:05, 1669781.44it/s] 9150732it [00:06, 1560034.55it/s] 9308206it [00:06, 1540692.77it/s] 9473742it [00:06, 1573540.13it/s] 9642199it [00:06, 1605773.52it/s] 9806829it [00:06, 1617505.29it/s] 9969098it [00:06, 1594832.01it/s] 10128988it [00:06, 1576964.42it/s] 10286981it [00:06, 1571988.95it/s] 10427202it [00:06, 1523251.39it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md.bai + merge_qc -folders aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1/aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1.md -output aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1/aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1/aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1/aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1_full_basic_stats.tsv