[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0fc41c-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (10953863) splicing reads. 237537 splicing junctions. 176661 known splicing junctions. 60876 novel splicing junctions.
sampling 10% (21907726) splicing reads. 285236 splicing junctions. 189298 known splicing junctions. 95938 novel splicing junctions.
sampling 15% (32861590) splicing reads. 317844 splicing junctions. 196148 known splicing junctions. 121696 novel splicing junctions.
sampling 20% (43815453) splicing reads. 342755 splicing junctions. 200586 known splicing junctions. 142169 novel splicing junctions.
sampling 25% (54769317) splicing reads. 363111 splicing junctions. 203863 known splicing junctions. 159248 novel splicing junctions.
sampling 30% (65723180) splicing reads. 379805 splicing junctions. 206347 known splicing junctions. 173458 novel splicing junctions.
sampling 35% (76677043) splicing reads. 393845 splicing junctions. 208358 known splicing junctions. 185487 novel splicing junctions.
sampling 40% (87630907) splicing reads. 405932 splicing junctions. 209911 known splicing junctions. 196021 novel splicing junctions.
sampling 45% (98584770) splicing reads. 416111 splicing junctions. 211210 known splicing junctions. 204901 novel splicing junctions.
sampling 50% (109538634) splicing reads. 424952 splicing junctions. 212243 known splicing junctions. 212709 novel splicing junctions.
sampling 55% (120492497) splicing reads. 432470 splicing junctions. 213120 known splicing junctions. 219350 novel splicing junctions.
sampling 60% (131446360) splicing reads. 439225 splicing junctions. 213884 known splicing junctions. 225341 novel splicing junctions.
sampling 65% (142400224) splicing reads. 444856 splicing junctions. 214525 known splicing junctions. 230331 novel splicing junctions.
sampling 70% (153354087) splicing reads. 449835 splicing junctions. 215075 known splicing junctions. 234760 novel splicing junctions.
sampling 75% (164307951) splicing reads. 454042 splicing junctions. 215551 known splicing junctions. 238491 novel splicing junctions.
sampling 80% (175261814) splicing reads. 457647 splicing junctions. 215946 known splicing junctions. 241701 novel splicing junctions.
sampling 85% (186215677) splicing reads. 460741 splicing junctions. 216247 known splicing junctions. 244494 novel splicing junctions.
sampling 90% (197169541) splicing reads. 463290 splicing junctions. 216515 known splicing junctions. 246775 novel splicing junctions.
sampling 95% (208123404) splicing reads. 465153 splicing junctions. 216695 known splicing junctions. 248458 novel splicing junctions.
sampling 100% (219077268) splicing reads. 466497 splicing junctions. 216832 known splicing junctions. 249665 novel splicing junctions.