MultiQC Report

A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

This report has been generated by the nf-core/rnafusion analysis pipeline. For information about how to interpret these results, please see the documentation.

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Report generated on 2026-05-15, 02:45 UTC based on data in: /tmp/nxf.hRgXfxDS8z

General Statistics

Showing ⁴⁸⁰/₄₈₀ rows and ⁵⁵/₆₂ columns.

Sample Name	Total Reads	Expected Distinct	Fragment Length Mean	Fragment Length Median	pct Mito est. counts	pct Ribo est. counts	pct Housekeeping est. counts	pct Male est. counts	pct Mito TPM	pct Ribo TPM	pct Housekeeping TPM	pct Male TPM	RNA contaminated	RNA contamination %	Reads	Frac Unmapped	Frac Dupes	Unique Reads	Frac Reads On Target	Frac Pairs On Target	Frac Reads Off Target	Frac Pairs Off Target	Reads On Target	Pairs On Target	Reads Off Target	Pairs Off Target	Mean Depth	Q50 Depth	Frac Bases Gt30X	Frac Bases Gt100X	N Q30	GC Bias	Frag Length	% Aligned	M Aligned	Insert Size	Mean Insert Size	Duplication	rRNA	mRNA	% Proper Pairs	% Aligned	M Aligned	% Duplication	% > Q30	Mb Q30 bases	M Reads After Filtering	GC content	% PF	% Adapter	% Dups	% GC	Average Read Length	Median Read Length	% Failed	M Seqs	% Dups	% GC	Average Read Length	Median Read Length	% Failed	M Seqs
FFPE_HD789_01_RNA_13_B23WHYVLT4_1	9999000000	298837526	168.7bp	153.0bp	0.02%	1.91%	0.86%	0.00%	0.04%	5.44%	1.20%	0.00%	Yes	65.10%	196898000	0.01%	0.32%	134735675	0.95%	0.97%	0.05%	0.03%	127575043	64574083	6080844	1721068	279.65	101.00	0.63%	0.50%	0.98	0.000				303bp	917bp	31.7%	0.0%	97.5%	91.2%	96.2%	94.7M	23.6%	97.6%	26314.3Mb	196.9M	53.6%	98.4%	40.0%
FFPE_HD789_01_RNA_13_B23WHYVLT4_1_1																																	168.7bp	98.0%	96.5M																87.8%	53%	151bp	151bp	18%	406.0M
FFPE_HD789_01_RNA_13_B23WHYVLT4_1_2																																																			86.3%	53%	151bp	151bp	18%	406.0M
FFPE_HD789_01_RNA_13_B23WHYVLT4_1_trimmed_1																																																			77.5%	53%	137bp	151bp	9%	98.4M	77.5%	53%	137bp	151bp	9%	98.4M
FFPE_HD789_01_RNA_13_B23WHYVLT4_1_trimmed_2																																																			76.5%	54%	137bp	151bp	9%	98.4M	76.5%	54%	137bp	151bp	9%	98.4M
FFPE_V4_0002_RNA_0001_B23WHYVLT4_1	9999000000	223400642	159.4bp	145.0bp	0.02%	2.13%	1.50%	0.00%	0.03%	5.72%	1.94%	0.00%	No	0.00%	196703800	0.01%	0.37%	123694749	0.94%	0.97%	0.05%	0.03%	116233013	58816561	6245334	1950028	241.86	51.00	0.54%	0.44%	0.98	0.000				242bp	695bp	37.3%	0.0%	97.0%	90.8%	95.9%	94.3M	26.8%	97.7%	25748.6Mb	196.7M	53.0%	98.4%	46.0%
FFPE_V4_0002_RNA_0001_B23WHYVLT4_1_1																																	159.4bp	97.2%	95.6M																90.1%	52%	151bp	151bp	18%	524.1M
FFPE_V4_0002_RNA_0001_B23WHYVLT4_1_2																																																			88.7%	53%	151bp	151bp	18%	524.1M
FFPE_V4_0002_RNA_0001_B23WHYVLT4_1_trimmed_1																																																			78.4%	52%	134bp	151bp	9%	98.4M	78.4%	52%	134bp	151bp	9%	98.4M
FFPE_V4_0002_RNA_0001_B23WHYVLT4_1_trimmed_2																																																			78.1%	53%	134bp	151bp	9%	98.4M	78.1%	53%	134bp	151bp	9%	98.4M
NTC_0001_0001_B23WHYVLT4_1	9999000000	45440	44.7bp	31.0bp	0.00%	0.00%	0.35%	0.00%	0.00%	0.00%	1.10%	0.00%	Skipped	N/A%	46656	0.64%	0.18%	38086	0.01%	0.04%	0.21%	0.96%	283	150	7821	3769	0.00	0.00	0.00%	0.00%	0.83	0.000				67bp	69bp	51.4%	0.0%	20.4%	4.8%	10.3%	0.0M	65.8%	84.3%	4.6Mb	0.0M	68.8%	2.4%	50.1%
NTC_0001_0001_B23WHYVLT4_1_1																																	44.7bp	12.4%	0.0M																98.7%	73%	151bp	151bp	45%	1.0M
NTC_0001_0001_B23WHYVLT4_1_2																																																			96.5%	98%	151bp	151bp	55%	1.0M
NTC_0001_0001_B23WHYVLT4_1_trimmed_1																																																			67.2%	64%	117bp	137bp	55%	0.0M	67.2%	64%	117bp	137bp	55%	0.0M
NTC_0001_0001_B23WHYVLT4_1_trimmed_2																																																			69.9%	73%	117bp	137bp	55%	0.0M	69.9%	73%	117bp	137bp	55%	0.0M
aih-tih-sc-0183f6-R1_B23WHYVLT4_1	9999000000	89242871	111.6bp	106.0bp	0.06%	1.24%	0.42%	0.00%	0.12%	3.68%	0.64%	0.00%	No	0.00%	196510412	0.01%	0.60%	78211362	0.89%	0.92%	0.09%	0.08%	69602575	34885964	7047673	3132679	100.83	43.00	0.56%	0.31%	0.98	0.000				109bp	149bp	60.7%	0.0%	96.1%	82.6%	92.9%	91.3M	34.1%	97.7%	21346.7Mb	196.5M	52.4%	98.3%	83.2%
aih-tih-sc-0183f6-R1_B23WHYVLT4_1_1																																	111.6bp	97.0%	95.3M																86.0%	51%	151bp	151bp	27%	218.9M
aih-tih-sc-0183f6-R1_B23WHYVLT4_1_2																																																			84.4%	52%	151bp	151bp	27%	218.9M
aih-tih-sc-0183f6-R1_B23WHYVLT4_1_trimmed_1																																																			78.6%	52%	111bp	112bp	9%	98.3M	78.6%	52%	111bp	112bp	9%	98.3M
aih-tih-sc-0183f6-R1_B23WHYVLT4_1_trimmed_2																																																			77.6%	52%	111bp	112bp	9%	98.3M	77.6%	52%	111bp	112bp	9%	98.3M
aih-tih-sc-027d27-R1_B23WHYVLT4_1	9999000000	188327931	131.1bp	122.0bp	0.02%	1.27%	0.45%	0.00%	0.04%	3.69%	0.67%	0.00%	Yes	0.62%	196604932	0.01%	0.38%	121367120	0.93%	0.95%	0.06%	0.05%	112885099	56777803	7084001	2787440	197.40	98.00	0.68%	0.50%	0.98	0.000				135bp	236bp	38.5%	0.0%	96.1%	87.8%	95.0%	93.3M	23.8%	97.7%	23626.5Mb	196.6M	54.0%	98.3%	66.4%
aih-tih-sc-027d27-R1_B23WHYVLT4_1_1																																	131.1bp	97.2%	95.5M																82.3%	52%	151bp	151bp	18%	264.1M
aih-tih-sc-027d27-R1_B23WHYVLT4_1_2																																																			80.3%	53%	151bp	151bp	18%	264.1M
aih-tih-sc-027d27-R1_B23WHYVLT4_1_trimmed_1																																																			72.5%	53%	123bp	127bp	9%	98.3M	72.5%	53%	123bp	127bp	9%	98.3M
aih-tih-sc-027d27-R1_B23WHYVLT4_1_trimmed_2																																																			71.1%	54%	123bp	127bp	9%	98.3M	71.1%	54%	123bp	127bp	9%	98.3M
aih-tih-sc-03bdd8-R1_B23WHYVLT4_1	9999000000	195905537	135.4bp	125.0bp	0.07%	1.01%	0.49%	0.00%	0.15%	3.14%	0.75%	0.00%	No	0.00%	196609000	0.01%	0.39%	120750708	0.94%	0.96%	0.05%	0.04%	113414892	57090949	5898718	2141657	206.94	95.00	0.67%	0.49%	0.98	0.000				151bp	296bp	38.9%	0.0%	96.8%	87.1%	94.7%	93.0M	24.9%	97.6%	24099.8Mb	196.6M	56.1%	98.3%	62.0%
aih-tih-sc-03bdd8-R1_B23WHYVLT4_1_1																																	135.4bp	97.7%	96.0M																89.2%	54%	151bp	151bp	18%	572.8M
aih-tih-sc-03bdd8-R1_B23WHYVLT4_1_2																																																			87.3%	56%	151bp	151bp	18%	572.8M
aih-tih-sc-03bdd8-R1_B23WHYVLT4_1_trimmed_1																																																			75.2%	55%	125bp	132bp	9%	98.3M	75.2%	55%	125bp	132bp	9%	98.3M
aih-tih-sc-03bdd8-R1_B23WHYVLT4_1_trimmed_2																																																			74.2%	56%	126bp	132bp	9%	98.3M	74.2%	56%	126bp	132bp	9%	98.3M
aih-tih-sc-070e1d-R1_B23WHYVLT4_1	9999000000	82896826	119.1bp	112.0bp	0.02%	1.55%	0.70%	0.00%	0.04%	4.13%	0.98%	0.00%	No	0.00%	196129140	0.01%	0.57%	85149285	0.92%	0.94%	0.07%	0.06%	78031777	39184969	5816397	2384424	123.80	50.00	0.58%	0.35%	0.98	0.000				124bp	216bp	57.0%	0.0%	96.8%	87.2%	94.7%	92.8M	34.3%	97.6%	22411.9Mb	196.1M	55.0%	98.1%	75.4%
aih-tih-sc-070e1d-R1_B23WHYVLT4_1_1																																	119.1bp	97.2%	95.3M																88.1%	53%	151bp	151bp	18%	282.0M
aih-tih-sc-070e1d-R1_B23WHYVLT4_1_2																																																			86.4%	55%	151bp	151bp	18%	282.0M
aih-tih-sc-070e1d-R1_B23WHYVLT4_1_trimmed_1																																																			78.9%	54%	117bp	117bp	9%	98.1M	78.9%	54%	117bp	117bp	9%	98.1M
aih-tih-sc-070e1d-R1_B23WHYVLT4_1_trimmed_2																																																			78.4%	55%	117bp	117bp	9%	98.1M	78.4%	55%	117bp	117bp	9%	98.1M
aih-tih-sc-099ac4-R1_B23WHYVLT4_1	9999000000	258205487	142.8bp	132.0bp	0.02%	0.48%	0.34%	0.00%	0.04%	1.55%	0.53%	0.00%	No	0.00%	196847122	0.01%	0.34%	129329310	0.94%	0.96%	0.05%	0.04%	121355684	61137094	6334866	2261227	231.85	84.00	0.64%	0.47%	0.98	0.000				156bp	327bp	34.6%	0.0%	97.0%	88.6%	95.2%	93.7M	22.8%	97.7%	24704.8Mb	196.8M	54.0%	98.4%	58.0%
aih-tih-sc-099ac4-R1_B23WHYVLT4_1_1																																	142.8bp	97.7%	96.2M																87.4%	53%	151bp	151bp	18%	457.9M
aih-tih-sc-099ac4-R1_B23WHYVLT4_1_2																																																			85.9%	54%	151bp	151bp	18%	457.9M
aih-tih-sc-099ac4-R1_B23WHYVLT4_1_trimmed_1																																																			75.8%	53%	128bp	137bp	9%	98.4M	75.8%	53%	128bp	137bp	9%	98.4M
aih-tih-sc-099ac4-R1_B23WHYVLT4_1_trimmed_2																																																			74.9%	54%	129bp	137bp	9%	98.4M	74.9%	54%	129bp	137bp	9%	98.4M
aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1	9999000000	245720980	137.3bp	126.0bp	0.02%	1.21%	0.70%	0.00%	0.05%	3.85%	1.09%	0.00%	No	0.00%	196848858	0.01%	0.37%	124899573	0.95%	0.97%	0.04%	0.03%	118516010	59684382	5216779	1752262	221.66	97.00	0.67%	0.50%	0.98	0.000				160bp	365bp	36.8%	0.0%	97.4%	87.5%	94.7%	93.2M	23.4%	97.7%	24252.2Mb	196.8M	54.8%	98.4%	60.5%
aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1_1																																	137.3bp	98.2%	96.7M																88.3%	54%	151bp	151bp	18%	508.5M
aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1_2																																																			87.0%	54%	151bp	151bp	18%	508.5M
aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1_trimmed_1																																																			75.8%	54%	126bp	137bp	9%	98.4M	75.8%	54%	126bp	137bp	9%	98.4M
aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1_trimmed_2																																																			75.2%	55%	126bp	137bp	9%	98.4M	75.2%	55%	126bp	137bp	9%	98.4M
aih-tih-sc-0fc41c-R1_B23WHYVLT4_1	9999000000	188908325	131.3bp	122.0bp	0.04%	0.66%	0.41%	0.00%	0.07%	2.08%	0.63%	0.00%	No	0.00%	196646954	0.01%	0.39%	119606908	0.93%	0.96%	0.05%	0.04%	111735487	56209425	6145450	2296817	197.51	79.00	0.63%	0.46%	0.98	0.000				134bp	236bp	39.5%	0.0%	96.9%	86.1%	94.2%	92.7M	24.0%	97.6%	23607.1Mb	196.6M	55.2%	98.3%	66.6%
aih-tih-sc-0fc41c-R1_B23WHYVLT4_1_1																																	131.3bp	97.8%	96.2M																82.2%	54%	151bp	151bp	18%	229.1M
aih-tih-sc-0fc41c-R1_B23WHYVLT4_1_2																																																			80.5%	55%	151bp	151bp	18%	229.1M
aih-tih-sc-0fc41c-R1_B23WHYVLT4_1_trimmed_1																																																			74.2%	54%	123bp	127bp	9%	98.3M	74.2%	54%	123bp	127bp	9%	98.3M
aih-tih-sc-0fc41c-R1_B23WHYVLT4_1_trimmed_2																																																			73.4%	55%	123bp	127bp	9%	98.3M	73.4%	55%	123bp	127bp	9%	98.3M
aih-tih-sc-13a2f6-R1_B23WHYVLT4_1	9999000000	52950048	116.5bp	111.0bp	0.03%	1.11%	0.49%	0.00%	0.06%	3.62%	0.80%	0.00%	No	0.00%	196417432	0.01%	0.72%	54876534	0.88%	0.92%	0.09%	0.08%	48104171	24095920	4942774	2169258	71.99	29.00	0.49%	0.20%	0.98	0.000				118bp	180bp	72.7%	0.0%	96.8%	86.9%	94.5%	92.8M	43.8%	97.6%	21938.9Mb	196.4M	54.5%	98.2%	79.7%
aih-tih-sc-13a2f6-R1_B23WHYVLT4_1_1																																	116.5bp	97.5%	95.8M																83.0%	53%	151bp	151bp	18%	106.1M
aih-tih-sc-13a2f6-R1_B23WHYVLT4_1_2																																																			81.2%	54%	151bp	151bp	27%	106.1M
aih-tih-sc-13a2f6-R1_B23WHYVLT4_1_trimmed_1																																																			82.4%	54%	114bp	112bp	9%	98.2M	82.4%	54%	114bp	112bp	9%	98.2M
aih-tih-sc-13a2f6-R1_B23WHYVLT4_1_trimmed_2																																																			81.7%	54%	115bp	112bp	9%	98.2M	81.7%	54%	115bp	112bp	9%	98.2M
aih-tih-sc-16054b-R1_B23WHYVLT4_1	9999000000	237823732	142.2bp	131.0bp	0.02%	0.77%	0.42%	0.00%	0.06%	2.59%	0.70%	0.00%	No	0.00%	196983676	0.01%	0.39%	120692323	0.94%	0.97%	0.05%	0.03%	113428971	57206655	5943947	2065338	217.73	98.00	0.67%	0.50%	0.98	0.000				185bp	423bp	39.0%	0.0%	96.6%	90.3%	95.8%	94.3M	26.5%	97.7%	24796.9Mb	197.0M	54.5%	98.5%	56.0%
aih-tih-sc-16054b-R1_B23WHYVLT4_1_1																																	142.2bp	97.9%	96.4M																86.1%	53%	151bp	151bp	18%	372.7M
aih-tih-sc-16054b-R1_B23WHYVLT4_1_2																																																			84.5%	54%	151bp	151bp	18%	372.7M
aih-tih-sc-16054b-R1_B23WHYVLT4_1_trimmed_1																																																			75.6%	54%	129bp	142bp	9%	98.5M	75.6%	54%	129bp	142bp	9%	98.5M
aih-tih-sc-16054b-R1_B23WHYVLT4_1_trimmed_2																																																			74.4%	54%	129bp	142bp	9%	98.5M	74.4%	54%	129bp	142bp	9%	98.5M
aih-tih-sc-176637-R1_B23WHYVLT4_1	9999000000	265943309	148.1bp	134.0bp	0.05%	0.77%	0.45%	0.00%	0.12%	2.39%	0.69%	0.00%	No	0.00%	196465992	0.01%	0.34%	129573675	0.94%	0.96%	0.05%	0.04%	121595580	61351429	6661014	2253552	236.51	104.00	0.68%	0.51%	0.98	0.000				179bp	441bp	34.3%	0.0%	96.6%	89.8%	95.6%	93.9M	22.5%	97.7%	24813.6Mb	196.5M	53.0%	98.2%	54.2%
aih-tih-sc-176637-R1_B23WHYVLT4_1_1																																	148.1bp	97.7%	96.0M																82.3%	52%	151bp	151bp	18%	193.1M
aih-tih-sc-176637-R1_B23WHYVLT4_1_2																																																			80.4%	53%	151bp	151bp	18%	193.1M
aih-tih-sc-176637-R1_B23WHYVLT4_1_trimmed_1																																																			76.4%	52%	129bp	142bp	9%	98.2M	76.4%	52%	129bp	142bp	9%	98.2M
aih-tih-sc-176637-R1_B23WHYVLT4_1_trimmed_2																																																			75.3%	53%	129bp	142bp	9%	98.2M	75.3%	53%	129bp	142bp	9%	98.2M
aih-tih-sc-18a6f2-R1_B23WHYVLT4_1	9999000000	65061613	117.3bp	110.0bp	0.04%	0.98%	0.48%	0.00%	0.08%	3.19%	0.77%	0.00%	No	0.00%	196719152	0.01%	0.65%	69299658	0.91%	0.94%	0.07%	0.06%	62906211	31560169	5138787	2182917	97.31	40.00	0.56%	0.29%	0.98	0.000				124bp	215bp	65.2%	0.0%	96.8%	90.1%	95.8%	94.2M	41.1%	97.7%	22216.7Mb	196.7M	54.2%	98.4%	77.4%
aih-tih-sc-18a6f2-R1_B23WHYVLT4_1_1																																	117.3bp	97.7%	96.1M																86.6%	53%	151bp	151bp	18%	196.4M
aih-tih-sc-18a6f2-R1_B23WHYVLT4_1_2																																																			84.7%	53%	151bp	151bp	18%	196.4M
aih-tih-sc-18a6f2-R1_B23WHYVLT4_1_trimmed_1																																																			80.4%	53%	115bp	117bp	9%	98.4M	80.4%	53%	115bp	117bp	9%	98.4M
aih-tih-sc-18a6f2-R1_B23WHYVLT4_1_trimmed_2																																																			79.4%	54%	116bp	117bp	9%	98.4M	79.4%	54%	116bp	117bp	9%	98.4M
aih-tih-sc-1ba302-R1_B23WHYVLT4_1	9999000000	230223442	147.3bp	135.0bp	0.03%	1.26%	0.59%	0.00%	0.07%	4.20%	0.94%	0.00%	No	0.00%	197245090	0.01%	0.38%	121580738	0.94%	0.96%	0.05%	0.04%	114192585	57637287	6146813	2120056	225.22	98.00	0.66%	0.50%	0.98	0.000				193bp	468bp	38.6%	0.0%	96.7%	89.6%	95.6%	94.3M	27.0%	97.7%	25189.5Mb	197.2M	54.0%	98.6%	52.9%
aih-tih-sc-1ba302-R1_B23WHYVLT4_1_1																																	147.3bp	97.9%	96.5M																88.3%	53%	151bp	151bp	18%	415.2M
aih-tih-sc-1ba302-R1_B23WHYVLT4_1_2																																																			86.8%	54%	151bp	151bp	18%	415.2M
aih-tih-sc-1ba302-R1_B23WHYVLT4_1_trimmed_1																																																			77.7%	53%	131bp	147bp	9%	98.6M	77.7%	53%	131bp	147bp	9%	98.6M
aih-tih-sc-1ba302-R1_B23WHYVLT4_1_trimmed_2																																																			76.4%	54%	131bp	147bp	9%	98.6M	76.4%	54%	131bp	147bp	9%	98.6M
aih-tih-sc-1d89ab-R1_B23WHYVLT4_1	9999000000	169156151	130.0bp	121.0bp	0.02%	0.79%	0.46%	0.00%	0.05%	2.55%	0.70%	0.00%	No	0.00%	196741702	0.01%	0.42%	113967065	0.93%	0.96%	0.05%	0.04%	106369401	53507983	6217449	2352766	187.46	85.00	0.65%	0.47%	0.98	0.000				141bp	268bp	42.4%	0.0%	96.7%	89.0%	95.3%	93.7M	25.7%	97.5%	23602.9Mb	196.7M	54.2%	98.4%	66.5%
aih-tih-sc-1d89ab-R1_B23WHYVLT4_1_1																																	130.0bp	97.4%	95.8M																85.8%	53%	151bp	151bp	18%	313.3M
aih-tih-sc-1d89ab-R1_B23WHYVLT4_1_2																																																			84.1%	54%	151bp	151bp	18%	313.3M
aih-tih-sc-1d89ab-R1_B23WHYVLT4_1_trimmed_1																																																			75.3%	53%	123bp	127bp	9%	98.4M	75.3%	53%	123bp	127bp	9%	98.4M
aih-tih-sc-1d89ab-R1_B23WHYVLT4_1_trimmed_2																																																			74.4%	54%	123bp	127bp	9%	98.4M	74.4%	54%	123bp	127bp	9%	98.4M
aih-tih-sc-1d9134-R1_B23WHYVLT4_1	9999000000	245259041	134.9bp	124.0bp	0.02%	0.90%	0.47%	0.00%	0.04%	3.06%	0.76%	0.00%	No	0.00%	197211186	0.01%	0.40%	117809243	0.94%	0.97%	0.05%	0.03%	111052910	55932318	5644271	2026618	205.77	85.00	0.65%	0.47%	0.98	0.000				174bp	437bp	40.5%	0.0%	97.1%	91.2%	96.1%	94.8M	26.6%	97.8%	24163.3Mb	197.2M	53.0%	98.6%	62.2%
aih-tih-sc-1d9134-R1_B23WHYVLT4_1_1																																	134.9bp	98.2%	96.8M																84.3%	52%	151bp	151bp	18%	241.1M
aih-tih-sc-1d9134-R1_B23WHYVLT4_1_2																																																			82.6%	53%	151bp	151bp	18%	241.1M
aih-tih-sc-1d9134-R1_B23WHYVLT4_1_trimmed_1																																																			77.3%	52%	125bp	132bp	9%	98.6M	77.3%	52%	125bp	132bp	9%	98.6M
aih-tih-sc-1d9134-R1_B23WHYVLT4_1_trimmed_2																																																			76.0%	53%	125bp	132bp	9%	98.6M	76.0%	53%	125bp	132bp	9%	98.6M
aih-tih-sc-20b7e4-R1_B23WHYVLT4_1	9999000000	56137633	112.5bp	107.0bp	0.01%	0.52%	0.33%	0.00%	0.03%	1.65%	0.51%	0.00%	No	0.00%	196118500	0.01%	0.75%	48690874	0.83%	0.87%	0.13%	0.13%	40620575	20333890	6376628	2931296	57.46	21.00	0.44%	0.16%	0.98	0.000				113bp	152bp	75.8%	0.0%	95.5%	85.9%	94.0%	92.2M	37.2%	97.6%	21417.0Mb	196.1M	54.2%	98.1%	82.1%
aih-tih-sc-20b7e4-R1_B23WHYVLT4_1_1																																	112.5bp	96.5%	94.7M																83.8%	53%	151bp	151bp	18%	113.3M
aih-tih-sc-20b7e4-R1_B23WHYVLT4_1_2																																																			81.9%	54%	151bp	151bp	27%	113.3M
aih-tih-sc-20b7e4-R1_B23WHYVLT4_1_trimmed_1																																																			82.6%	53%	112bp	112bp	9%	98.1M	82.6%	53%	112bp	112bp	9%	98.1M
aih-tih-sc-20b7e4-R1_B23WHYVLT4_1_trimmed_2																																																			81.7%	54%	112bp	112bp	9%	98.1M	81.7%	54%	112bp	112bp	9%	98.1M
aih-tih-sc-2a5468-R1_B23WHYVLT4_1	9999000000	240629194	141.5bp	129.0bp	0.04%	1.28%	0.56%	0.00%	0.09%	3.78%	0.85%	0.00%	No	0.00%	195820002	0.01%	0.35%	127279240	0.94%	0.97%	0.05%	0.03%	119737446	60321977	6205779	2104766	226.37	98.00	0.66%	0.50%	0.98	0.000				164bp	399bp	35.2%	0.0%	96.7%	88.8%	95.3%	93.3M	23.6%	97.6%	24308.2Mb	195.8M	52.6%	97.9%	57.9%
aih-tih-sc-2a5468-R1_B23WHYVLT4_1_1																																	141.5bp	97.8%	95.7M																89.0%	51%	151bp	151bp	27%	525.7M
aih-tih-sc-2a5468-R1_B23WHYVLT4_1_2																																																			86.8%	53%	151bp	151bp	18%	525.7M
aih-tih-sc-2a5468-R1_B23WHYVLT4_1_trimmed_1																																																			76.6%	52%	127bp	137bp	18%	97.9M	76.6%	52%	127bp	137bp	18%	97.9M
aih-tih-sc-2a5468-R1_B23WHYVLT4_1_trimmed_2																																																			75.2%	52%	127bp	137bp	9%	97.9M	75.2%	52%	127bp	137bp	9%	97.9M
aih-tih-sc-2f84cc-R1_B23WHYVLT4_1	9999000000	107438048	129.3bp	122.0bp	0.01%	1.15%	0.49%	0.00%	0.03%	3.54%	0.75%	0.00%	No	0.00%	196287862	0.01%	0.50%	97513387	0.90%	0.93%	0.08%	0.07%	87936966	44171916	7700900	3255613	146.91	55.00	0.59%	0.39%	0.98	0.000				128bp	187bp	50.8%	0.0%	95.5%	87.0%	94.5%	92.8M	31.5%	97.6%	23347.4Mb	196.3M	53.2%	98.1%	70.0%
aih-tih-sc-2f84cc-R1_B23WHYVLT4_1_1																																	129.3bp	96.5%	94.7M																76.7%	52%	151bp	151bp	18%	124.4M
aih-tih-sc-2f84cc-R1_B23WHYVLT4_1_2																																																			74.7%	53%	151bp	151bp	18%	124.4M
aih-tih-sc-2f84cc-R1_B23WHYVLT4_1_trimmed_1																																																			74.0%	52%	122bp	127bp	9%	98.1M	74.0%	52%	122bp	127bp	9%	98.1M
aih-tih-sc-2f84cc-R1_B23WHYVLT4_1_trimmed_2																																																			73.1%	53%	122bp	127bp	9%	98.1M	73.1%	53%	122bp	127bp	9%	98.1M
aih-tih-sc-3376ed-R1_B23WHYVLT4_1	9999000000	205678194	154.1bp	140.0bp	0.04%	0.78%	0.82%	0.00%	0.09%	2.39%	1.09%	0.00%	No	0.00%	196555484	0.01%	0.38%	122246312	0.95%	0.97%	0.04%	0.03%	115871246	58502172	5187922	1555057	235.90	81.00	0.62%	0.47%	0.98	0.000				211bp	589bp	38.0%	0.0%	97.7%	90.6%	95.8%	94.2M	27.5%	97.7%	25492.9Mb	196.6M	53.8%	98.3%	49.0%
aih-tih-sc-3376ed-R1_B23WHYVLT4_1_1																																	154.1bp	98.3%	96.6M																88.3%	52%	151bp	151bp	18%	393.6M
aih-tih-sc-3376ed-R1_B23WHYVLT4_1_2																																																			86.7%	54%	151bp	151bp	18%	393.6M
aih-tih-sc-3376ed-R1_B23WHYVLT4_1_trimmed_1																																																			77.6%	53%	133bp	151bp	9%	98.3M	77.6%	53%	133bp	151bp	9%	98.3M
aih-tih-sc-3376ed-R1_B23WHYVLT4_1_trimmed_2																																																			77.1%	54%	133bp	151bp	9%	98.3M	77.1%	54%	133bp	151bp	9%	98.3M
aih-tih-sc-37dd87-R1_B23WHYVLT4_1	9999000000	301288574	141.1bp	129.0bp	0.04%	1.01%	0.65%	0.00%	0.10%	3.10%	0.93%	0.00%	No	0.00%	197053794	0.01%	0.32%	134475953	0.95%	0.97%	0.05%	0.03%	127216154	64134930	6074219	2050694	242.96	105.00	0.66%	0.51%	0.98	0.000				163bp	415bp	31.9%	0.0%	97.3%	90.2%	95.8%	94.4M	21.1%	97.8%	24529.3Mb	197.1M	52.2%	98.5%	57.8%
aih-tih-sc-37dd87-R1_B23WHYVLT4_1_1																																	141.1bp	98.0%	96.6M																85.2%	51%	151bp	151bp	18%	312.9M
aih-tih-sc-37dd87-R1_B23WHYVLT4_1_2																																																			83.3%	52%	151bp	151bp	18%	312.9M
aih-tih-sc-37dd87-R1_B23WHYVLT4_1_trimmed_1																																																			76.2%	51%	127bp	137bp	9%	98.5M	76.2%	51%	127bp	137bp	9%	98.5M
aih-tih-sc-37dd87-R1_B23WHYVLT4_1_trimmed_2																																																			74.6%	52%	127bp	137bp	9%	98.5M	74.6%	52%	127bp	137bp	9%	98.5M
aih-tih-sc-3924cb-R1_B23WHYVLT4_1	9999000000	264567315	137.3bp	127.0bp	0.03%	0.72%	0.50%	0.00%	0.06%	2.24%	0.72%	0.00%	No	0.00%	196790234	0.01%	0.36%	125793175	0.94%	0.97%	0.05%	0.03%	118753782	59815483	5834672	2036743	222.04	93.00	0.65%	0.49%	0.98	0.000				163bp	382bp	36.3%	0.0%	97.0%	91.1%	96.1%	94.5M	22.8%	97.5%	24335.3Mb	196.8M	53.2%	98.4%	60.1%
aih-tih-sc-3924cb-R1_B23WHYVLT4_1_1																																	137.3bp	98.1%	96.5M																85.5%	52%	151bp	151bp	18%	314.0M
aih-tih-sc-3924cb-R1_B23WHYVLT4_1_2																																																			83.9%	53%	151bp	151bp	18%	314.0M
aih-tih-sc-3924cb-R1_B23WHYVLT4_1_trimmed_1																																																			76.2%	52%	127bp	137bp	9%	98.4M	76.2%	52%	127bp	137bp	9%	98.4M
aih-tih-sc-3924cb-R1_B23WHYVLT4_1_trimmed_2																																																			75.1%	53%	127bp	137bp	9%	98.4M	75.1%	53%	127bp	137bp	9%	98.4M
aih-tih-sc-3b3c1e-R1_B23WHYVLT4_1	9999000000	137823223	131.7bp	122.0bp	0.03%	1.04%	0.39%	0.00%	0.06%	3.25%	0.60%	0.00%	No	0.00%	196755142	0.01%	0.45%	108432036	0.93%	0.95%	0.06%	0.05%	100701146	50659104	6339679	2479813	177.03	77.00	0.65%	0.45%	0.98	0.000				134bp	240bp	45.2%	0.0%	96.6%	89.4%	95.5%	94.0M	28.9%	97.7%	23707.0Mb	196.8M	52.8%	98.4%	66.1%
aih-tih-sc-3b3c1e-R1_B23WHYVLT4_1_1																																	131.7bp	97.6%	96.0M																86.4%	51%	151bp	151bp	18%	313.9M
aih-tih-sc-3b3c1e-R1_B23WHYVLT4_1_2																																																			84.5%	53%	151bp	151bp	18%	313.9M
aih-tih-sc-3b3c1e-R1_B23WHYVLT4_1_trimmed_1																																																			76.0%	52%	123bp	127bp	18%	98.4M	76.0%	52%	123bp	127bp	18%	98.4M
aih-tih-sc-3b3c1e-R1_B23WHYVLT4_1_trimmed_2																																																			74.6%	53%	123bp	127bp	9%	98.4M	74.6%	53%	123bp	127bp	9%	98.4M
aih-tih-sc-3e91d2-R1_B23WHYVLT4_1	9999000000	258602220	147.5bp	135.0bp	0.02%	1.26%	0.72%	0.00%	0.03%	3.67%	1.04%	0.00%	No	0.00%	196813766	0.01%	0.36%	126582046	0.94%	0.97%	0.04%	0.03%	119247933	60207024	5671701	1805828	235.47	97.00	0.67%	0.50%	0.98	0.000				191bp	512bp	36.0%	0.0%	97.1%	88.9%	94.9%	93.4M	23.1%	97.7%	25098.2Mb	196.8M	53.1%	98.4%	53.5%
aih-tih-sc-3e91d2-R1_B23WHYVLT4_1_1																																	147.5bp	96.2%	94.7M																86.0%	52%	151bp	151bp	18%	418.3M
aih-tih-sc-3e91d2-R1_B23WHYVLT4_1_2																																																			84.4%	53%	151bp	151bp	18%	418.3M
aih-tih-sc-3e91d2-R1_B23WHYVLT4_1_trimmed_1																																																			74.4%	52%	130bp	147bp	9%	98.4M	74.4%	52%	130bp	147bp	9%	98.4M
aih-tih-sc-3e91d2-R1_B23WHYVLT4_1_trimmed_2																																																			73.4%	53%	131bp	147bp	9%	98.4M	73.4%	53%	131bp	147bp	9%	98.4M
aih-tih-sc-445ab1-R1_B23WHYVLT4_1	9999000000	179850567	152.3bp	140.0bp	0.01%	0.82%	0.55%	0.00%	0.04%	2.68%	0.85%	0.00%	No	0.00%	196595754	0.01%	0.41%	116609273	0.94%	0.96%	0.05%	0.04%	109303817	55139945	5991665	2072604	217.65	97.00	0.68%	0.50%	0.98	0.000				188bp	439bp	41.0%	0.0%	96.9%	89.9%	95.8%	94.2M	29.3%	97.7%	25387.3Mb	196.6M	54.7%	98.3%	50.8%
aih-tih-sc-445ab1-R1_B23WHYVLT4_1_1																																	152.3bp	97.8%	96.2M																86.8%	54%	151bp	151bp	18%	337.0M
aih-tih-sc-445ab1-R1_B23WHYVLT4_1_2																																																			85.3%	54%	151bp	151bp	18%	337.0M
aih-tih-sc-445ab1-R1_B23WHYVLT4_1_trimmed_1																																																			76.5%	54%	132bp	147bp	9%	98.3M	76.5%	54%	132bp	147bp	9%	98.3M
aih-tih-sc-445ab1-R1_B23WHYVLT4_1_trimmed_2																																																			75.8%	55%	132bp	147bp	9%	98.3M	75.8%	55%	132bp	147bp	9%	98.3M
aih-tih-sc-446670-R1_B23WHYVLT4_1	9999000000	180483822	144.9bp	135.0bp	0.02%	0.74%	0.81%	0.00%	0.03%	2.29%	1.18%	0.00%	No	0.00%	196624464	0.01%	0.43%	112809730	0.94%	0.97%	0.05%	0.03%	106001135	53437781	5203184	1746167	204.92	70.00	0.62%	0.44%	0.98	0.000				164bp	346bp	43.0%	0.0%	97.2%	87.2%	94.3%	92.7M	28.0%	97.6%	25036.6Mb	196.6M	55.4%	98.3%	55.7%
aih-tih-sc-446670-R1_B23WHYVLT4_1_1																																	144.9bp	98.0%	96.4M																83.0%	54%	151bp	151bp	18%	225.8M
aih-tih-sc-446670-R1_B23WHYVLT4_1_2																																																			81.4%	55%	151bp	151bp	18%	225.8M
aih-tih-sc-446670-R1_B23WHYVLT4_1_trimmed_1																																																			75.7%	55%	130bp	142bp	9%	98.3M	75.7%	55%	130bp	142bp	9%	98.3M
aih-tih-sc-446670-R1_B23WHYVLT4_1_trimmed_2																																																			75.1%	55%	131bp	142bp	9%	98.3M	75.1%	55%	131bp	142bp	9%	98.3M
aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1	9999000000	152591849	128.2bp	120.0bp	0.02%	1.10%	0.32%	0.00%	0.04%	3.46%	0.52%	0.00%	No	0.00%	196793128	0.01%	0.44%	111041457	0.92%	0.94%	0.07%	0.06%	102179565	51367026	7508157	3083311	175.08	83.00	0.66%	0.46%	0.98	0.000				130bp	203bp	43.9%	0.0%	96.0%	86.6%	94.3%	92.7M	27.9%	97.7%	23542.3Mb	196.8M	52.4%	98.4%	68.7%
aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1_1																																	128.2bp	97.1%	95.6M																82.0%	51%	151bp	151bp	18%	208.6M
aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1_2																																																			80.2%	52%	151bp	151bp	27%	208.6M
aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1_trimmed_1																																																			74.5%	52%	122bp	127bp	9%	98.4M	74.5%	52%	122bp	127bp	9%	98.4M
aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1_trimmed_2																																																			73.5%	52%	123bp	127bp	9%	98.4M	73.5%	52%	123bp	127bp	9%	98.4M
aih-tih-sc-4dcd88-R1_B23WHYVLT4_1	9999000000	250898850	146.2bp	133.0bp	0.02%	0.67%	0.44%	0.00%	0.05%	2.07%	0.67%	0.00%	No	0.00%	196921172	0.01%	0.34%	130214479	0.94%	0.97%	0.05%	0.03%	122789519	61998999	6163795	1994209	238.61	109.00	0.69%	0.52%	0.98	0.000				167bp	366bp	34.1%	0.0%	96.5%	90.1%	95.8%	94.3M	22.7%	97.5%	24881.6Mb	196.9M	54.9%	98.5%	54.6%
aih-tih-sc-4dcd88-R1_B23WHYVLT4_1_1																																	146.2bp	97.8%	96.3M																86.4%	53%	151bp	151bp	18%	380.0M
aih-tih-sc-4dcd88-R1_B23WHYVLT4_1_2																																																			84.6%	55%	151bp	151bp	18%	380.0M
aih-tih-sc-4dcd88-R1_B23WHYVLT4_1_trimmed_1																																																			75.2%	54%	129bp	142bp	9%	98.5M	75.2%	54%	129bp	142bp	9%	98.5M
aih-tih-sc-4dcd88-R1_B23WHYVLT4_1_trimmed_2																																																			73.7%	55%	130bp	142bp	9%	98.5M	73.7%	55%	130bp	142bp	9%	98.5M
aih-tih-sc-4f4d6e-R1_B23WHYVLT4_1	9999000000	193748648	136.6bp	125.0bp	0.02%	1.16%	0.47%	0.00%	0.05%	3.75%	0.76%	0.00%	No	0.00%	197535306	0.01%	0.42%	113690713	0.94%	0.97%	0.05%	0.03%	107133498	54070904	5515814	1891511	200.48	84.00	0.65%	0.47%	0.98	0.000				169bp	407bp	42.7%	0.0%	96.8%	91.9%	96.3%	95.1M	28.1%	97.7%	24432.8Mb	197.5M	52.7%	98.8%	59.9%
aih-tih-sc-4f4d6e-R1_B23WHYVLT4_1_1																																	136.6bp	98.0%	96.8M																84.5%	51%	151bp	151bp	27%	218.5M
aih-tih-sc-4f4d6e-R1_B23WHYVLT4_1_2																																																			82.5%	53%	151bp	151bp	18%	218.5M
aih-tih-sc-4f4d6e-R1_B23WHYVLT4_1_trimmed_1																																																			78.1%	52%	126bp	137bp	18%	98.8M	78.1%	52%	126bp	137bp	18%	98.8M
aih-tih-sc-4f4d6e-R1_B23WHYVLT4_1_trimmed_2																																																			76.2%	53%	127bp	137bp	9%	98.8M	76.2%	53%	127bp	137bp	9%	98.8M
aih-tih-sc-505123-R1_B23WHYVLT4_1	9999000000	121081324	117.2bp	111.0bp	0.01%	0.50%	0.30%	0.00%	0.03%	1.61%	0.44%	0.00%	No	0.00%	196411616	0.01%	0.51%	96898418	0.90%	0.92%	0.09%	0.08%	86917761	43586195	8615497	3831393	133.02	60.00	0.61%	0.39%	0.98	0.000				114bp	145bp	51.0%	0.0%	94.9%	87.2%	94.7%	93.0M	31.0%	97.7%	22045.2Mb	196.4M	52.7%	98.2%	78.5%
aih-tih-sc-505123-R1_B23WHYVLT4_1_1																																	117.2bp	96.3%	94.6M																77.5%	51%	151bp	151bp	18%	135.8M
aih-tih-sc-505123-R1_B23WHYVLT4_1_2																																																			75.2%	53%	151bp	151bp	18%	135.8M
aih-tih-sc-505123-R1_B23WHYVLT4_1_trimmed_1																																																			73.9%	52%	115bp	117bp	9%	98.2M	73.9%	52%	115bp	117bp	9%	98.2M
aih-tih-sc-505123-R1_B23WHYVLT4_1_trimmed_2																																																			72.5%	52%	115bp	117bp	9%	98.2M	72.5%	52%	115bp	117bp	9%	98.2M
aih-tih-sc-51d534-R1_B23WHYVLT4_1	9999000000	239008063	142.7bp	131.0bp	0.03%	1.30%	0.63%	0.00%	0.06%	3.93%	0.94%	0.00%	No	0.00%	196848160	0.01%	0.35%	128190101	0.95%	0.97%	0.04%	0.03%	121425784	61207420	5642836	1889833	231.47	97.00	0.66%	0.50%	0.98	0.000				164bp	381bp	35.1%	0.0%	97.2%	87.2%	94.5%	93.1M	23.6%	97.7%	24645.1Mb	196.8M	53.9%	98.4%	57.5%
aih-tih-sc-51d534-R1_B23WHYVLT4_1_1																																	142.7bp	98.1%	96.6M																84.8%	52%	151bp	151bp	18%	276.2M
aih-tih-sc-51d534-R1_B23WHYVLT4_1_2																																																			83.3%	53%	151bp	151bp	18%	276.2M
aih-tih-sc-51d534-R1_B23WHYVLT4_1_trimmed_1																																																			76.1%	53%	128bp	137bp	9%	98.4M	76.1%	53%	128bp	137bp	9%	98.4M
aih-tih-sc-51d534-R1_B23WHYVLT4_1_trimmed_2																																																			75.3%	54%	128bp	137bp	9%	98.4M	75.3%	54%	128bp	137bp	9%	98.4M
aih-tih-sc-51ee59-R1_B23WHYVLT4_1	9999000000	255322891	146.9bp	134.0bp	0.02%	1.11%	0.48%	0.00%	0.05%	3.55%	0.72%	0.00%	No	0.00%	196991568	0.01%	0.35%	127853954	0.94%	0.97%	0.05%	0.03%	120762021	60952332	5910889	1950028	237.10	108.00	0.69%	0.52%	0.98	0.000				182bp	489bp	35.3%	0.0%	97.1%	91.3%	96.2%	94.7M	24.1%	97.7%	25032.0Mb	197.0M	53.3%	98.5%	54.2%
aih-tih-sc-51ee59-R1_B23WHYVLT4_1_1																																	146.9bp	98.0%	96.6M																86.6%	52%	151bp	151bp	27%	383.0M
aih-tih-sc-51ee59-R1_B23WHYVLT4_1_2																																																			85.0%	53%	151bp	151bp	18%	383.0M
aih-tih-sc-51ee59-R1_B23WHYVLT4_1_trimmed_1																																																			76.0%	52%	130bp	142bp	18%	98.5M	76.0%	52%	130bp	142bp	18%	98.5M
aih-tih-sc-51ee59-R1_B23WHYVLT4_1_trimmed_2																																																			74.7%	53%	130bp	142bp	9%	98.5M	74.7%	53%	130bp	142bp	9%	98.5M
aih-tih-sc-522d83-R1_B23WHYVLT4_1	9999000000	239428181	144.1bp	132.0bp	0.03%	1.31%	0.67%	0.00%	0.07%	3.93%	1.00%	0.00%	No	0.00%	196363658	0.01%	0.35%	127731327	0.95%	0.97%	0.04%	0.03%	120788716	60863364	5683774	1864597	234.25	98.00	0.67%	0.50%	0.98	0.000				176bp	408bp	35.2%	0.0%	97.2%	88.9%	95.3%	93.5M	23.3%	97.6%	24786.9Mb	196.4M	55.8%	98.2%	55.1%
aih-tih-sc-522d83-R1_B23WHYVLT4_1_1																																	144.1bp	97.9%	96.1M																85.5%	54%	151bp	151bp	18%	320.5M
aih-tih-sc-522d83-R1_B23WHYVLT4_1_2																																																			83.7%	55%	151bp	151bp	18%	320.5M
aih-tih-sc-522d83-R1_B23WHYVLT4_1_trimmed_1																																																			75.6%	55%	129bp	142bp	9%	98.2M	75.6%	55%	129bp	142bp	9%	98.2M
aih-tih-sc-522d83-R1_B23WHYVLT4_1_trimmed_2																																																			74.8%	56%	129bp	142bp	9%	98.2M	74.8%	56%	129bp	142bp	9%	98.2M
aih-tih-sc-5c902f-R1_B23WHYVLT4_1	9999000000	56759941	122.1bp	116.0bp	0.01%	1.19%	0.41%	0.00%	0.03%	3.89%	0.73%	0.00%	No	0.00%	195558468	0.01%	0.67%	64386539	0.89%	0.93%	0.08%	0.07%	57006767	28575274	5254410	2243947	90.68	30.00	0.50%	0.24%	0.98	0.000				124bp	176bp	67.8%	0.0%	96.5%	81.5%	92.2%	90.2M	42.2%	97.4%	22671.9Mb	195.6M	58.8%	97.8%	73.6%
aih-tih-sc-5c902f-R1_B23WHYVLT4_1_1																																	122.1bp	97.1%	95.0M																88.3%	57%	151bp	151bp	27%	225.9M
aih-tih-sc-5c902f-R1_B23WHYVLT4_1_2																																																			86.2%	58%	151bp	151bp	27%	225.9M
aih-tih-sc-5c902f-R1_B23WHYVLT4_1_trimmed_1																																																			81.1%	58%	119bp	122bp	9%	97.8M	81.1%	58%	119bp	122bp	9%	97.8M
aih-tih-sc-5c902f-R1_B23WHYVLT4_1_trimmed_2																																																			80.3%	59%	119bp	122bp	9%	97.8M	80.3%	59%	119bp	122bp	9%	97.8M
aih-tih-sc-5ef3cb-R1_B23WHYVLT4_1	9999000000	71159231	138.4bp	128.0bp	0.01%	0.16%	0.31%	0.00%	0.02%	0.47%	0.48%	0.00%	No	0.00%	195969198	0.01%	0.60%	77537831	0.89%	0.93%	0.08%	0.07%	69121208	34856729	6589130	2634202	120.10	30.00	0.50%	0.30%	0.98	0.000				139bp	204bp	61.0%	0.0%	94.1%	87.3%	95.0%	93.0M	41.4%	97.5%	24134.7Mb	196.0M	57.4%	98.0%	60.5%
aih-tih-sc-5ef3cb-R1_B23WHYVLT4_1_1																																	138.4bp	96.2%	94.2M																88.9%	56%	151bp	151bp	18%	280.9M
aih-tih-sc-5ef3cb-R1_B23WHYVLT4_1_2																																																			86.9%	57%	151bp	151bp	18%	280.9M
aih-tih-sc-5ef3cb-R1_B23WHYVLT4_1_trimmed_1																																																			80.1%	57%	126bp	137bp	9%	98.0M	80.1%	57%	126bp	137bp	9%	98.0M
aih-tih-sc-5ef3cb-R1_B23WHYVLT4_1_trimmed_2																																																			79.4%	57%	126bp	137bp	9%	98.0M	79.4%	57%	126bp	137bp	9%	98.0M
aih-tih-sc-60f9b9-R1_B23WHYVLT4_1	9999000000	115857010	131.9bp	123.0bp	0.02%	1.71%	0.41%	0.00%	0.04%	5.20%	0.72%	0.00%	No	0.00%	196535824	0.01%	0.50%	98584223	0.92%	0.95%	0.06%	0.05%	90841348	45688158	6049907	2381789	157.95	66.00	0.62%	0.42%	0.98	0.000				137bp	241bp	50.3%	0.0%	96.7%	86.7%	94.4%	92.8M	32.6%	97.7%	23815.4Mb	196.5M	52.9%	98.3%	66.6%
aih-tih-sc-60f9b9-R1_B23WHYVLT4_1_1																																	131.9bp	97.7%	96.0M																79.9%	52%	151bp	151bp	27%	130.0M
aih-tih-sc-60f9b9-R1_B23WHYVLT4_1_2																																																			77.9%	53%	151bp	151bp	18%	130.0M
aih-tih-sc-60f9b9-R1_B23WHYVLT4_1_trimmed_1																																																			77.1%	52%	124bp	127bp	18%	98.3M	77.1%	52%	124bp	127bp	18%	98.3M
aih-tih-sc-60f9b9-R1_B23WHYVLT4_1_trimmed_2																																																			76.1%	53%	124bp	127bp	9%	98.3M	76.1%	53%	124bp	127bp	9%	98.3M
aih-tih-sc-6284ec-R1_B23WHYVLT4_1	9999000000	191219410	136.8bp	127.0bp	0.02%	1.03%	0.40%	0.00%	0.05%	3.21%	0.64%	0.00%	No	0.00%	196981462	0.01%	0.39%	119970994	0.94%	0.96%	0.05%	0.04%	112209720	56514985	6407808	2400469	205.48	97.00	0.68%	0.49%	0.98	0.000				152bp	324bp	39.4%	0.0%	96.5%	89.6%	95.5%	94.1M	25.3%	97.7%	24352.6Mb	197.0M	52.5%	98.5%	61.8%
aih-tih-sc-6284ec-R1_B23WHYVLT4_1_1																																	136.8bp	97.7%	96.2M																84.0%	52%	151bp	151bp	18%	280.1M
aih-tih-sc-6284ec-R1_B23WHYVLT4_1_2																																																			82.2%	52%	151bp	151bp	18%	280.1M
aih-tih-sc-6284ec-R1_B23WHYVLT4_1_trimmed_1																																																			74.6%	52%	126bp	132bp	9%	98.5M	74.6%	52%	126bp	132bp	9%	98.5M
aih-tih-sc-6284ec-R1_B23WHYVLT4_1_trimmed_2																																																			73.2%	52%	127bp	132bp	9%	98.5M	73.2%	52%	127bp	132bp	9%	98.5M
aih-tih-sc-62dadb-R1_B23WHYVLT4_1	9999000000	72845619	122.0bp	114.0bp	0.02%	0.76%	0.38%	0.00%	0.04%	2.38%	0.59%	0.00%	No	0.00%	179706190	0.01%	0.59%	74162037	0.89%	0.92%	0.09%	0.08%	66183956	33208812	6329328	2740984	104.63	50.00	0.60%	0.33%	0.98	0.000				120bp	170bp	59.3%	0.0%	95.6%	86.2%	94.4%	84.8M	36.4%	97.6%	20561.1Mb	179.7M	54.8%	98.1%	75.0%
aih-tih-sc-62dadb-R1_B23WHYVLT4_1_1																																	122.0bp	96.7%	86.9M																75.7%	53%	151bp	151bp	18%	91.6M
aih-tih-sc-62dadb-R1_B23WHYVLT4_1_2																																																			73.7%	54%	151bp	151bp	18%	91.6M
aih-tih-sc-62dadb-R1_B23WHYVLT4_1_trimmed_1																																																			75.6%	54%	117bp	117bp	9%	89.9M	75.6%	54%	117bp	117bp	9%	89.9M
aih-tih-sc-62dadb-R1_B23WHYVLT4_1_trimmed_2																																																			74.7%	55%	117bp	117bp	9%	89.9M	74.7%	55%	117bp	117bp	9%	89.9M
aih-tih-sc-63b0e7-R1_B23WHYVLT4_1	9999000000	253854435	140.4bp	128.0bp	0.02%	0.92%	0.47%	0.00%	0.05%	2.85%	0.69%	0.00%	No	0.00%	196773806	0.01%	0.34%	129673203	0.94%	0.97%	0.05%	0.03%	121991426	61532076	6311272	2169389	229.18	115.00	0.71%	0.53%	0.98	0.000				157bp	368bp	34.3%	0.0%	96.6%	90.3%	95.8%	94.2M	22.6%	97.7%	24414.9Mb	196.8M	53.0%	98.4%	58.6%
aih-tih-sc-63b0e7-R1_B23WHYVLT4_1_1																																	140.4bp	97.9%	96.3M																84.5%	52%	151bp	151bp	27%	321.4M
aih-tih-sc-63b0e7-R1_B23WHYVLT4_1_2																																																			83.0%	53%	151bp	151bp	18%	321.4M
aih-tih-sc-63b0e7-R1_B23WHYVLT4_1_trimmed_1																																																			74.3%	52%	127bp	137bp	18%	98.4M	74.3%	52%	127bp	137bp	18%	98.4M
aih-tih-sc-63b0e7-R1_B23WHYVLT4_1_trimmed_2																																																			73.1%	53%	127bp	137bp	9%	98.4M	73.1%	53%	127bp	137bp	9%	98.4M
aih-tih-sc-63c8b4-R1_B23WHYVLT4_1	9999000000	117334067	128.6bp	119.0bp	0.02%	0.88%	0.38%	0.00%	0.04%	2.94%	0.63%	0.00%	No	0.00%	196666082	0.01%	0.52%	94513251	0.93%	0.96%	0.05%	0.04%	87967539	44256767	5123154	1937751	155.56	65.00	0.63%	0.41%	0.98	0.000				154bp	321bp	52.3%	0.0%	97.1%	90.8%	96.0%	94.4M	30.7%	97.6%	23614.4Mb	196.7M	54.7%	98.3%	66.7%
aih-tih-sc-63c8b4-R1_B23WHYVLT4_1_1																																	128.6bp	97.9%	96.3M																86.4%	53%	151bp	151bp	27%	296.3M
aih-tih-sc-63c8b4-R1_B23WHYVLT4_1_2																																																			84.4%	54%	151bp	151bp	18%	296.3M
aih-tih-sc-63c8b4-R1_B23WHYVLT4_1_trimmed_1																																																			76.6%	54%	123bp	127bp	27%	98.3M	76.6%	54%	123bp	127bp	27%	98.3M
aih-tih-sc-63c8b4-R1_B23WHYVLT4_1_trimmed_2																																																			75.1%	54%	123bp	127bp	9%	98.3M	75.1%	54%	123bp	127bp	9%	98.3M
aih-tih-sc-6473fb-R1_B23WHYVLT4_1	9999000000	68057240	127.0bp	119.0bp	0.07%	0.94%	0.43%	0.00%	0.16%	2.96%	0.68%	0.00%	No	0.00%	196485444	0.01%	0.63%	72525368	0.92%	0.95%	0.06%	0.05%	66501287	33384921	4651414	1883856	109.47	45.00	0.57%	0.31%	0.98	0.000				128bp	199bp	63.5%	0.0%	96.8%	88.1%	94.9%	93.2M	41.6%	97.7%	23371.6Mb	196.5M	54.4%	98.2%	70.2%
aih-tih-sc-6473fb-R1_B23WHYVLT4_1_1																																	127.0bp	97.9%	96.1M																87.7%	53%	151bp	151bp	18%	238.4M
aih-tih-sc-6473fb-R1_B23WHYVLT4_1_2																																																			86.1%	54%	151bp	151bp	18%	238.4M
aih-tih-sc-6473fb-R1_B23WHYVLT4_1_trimmed_1																																																			79.7%	54%	122bp	127bp	9%	98.2M	79.7%	54%	122bp	127bp	9%	98.2M
aih-tih-sc-6473fb-R1_B23WHYVLT4_1_trimmed_2																																																			78.9%	54%	122bp	127bp	9%	98.2M	78.9%	54%	122bp	127bp	9%	98.2M
aih-tih-sc-6e7384-R1_B23WHYVLT4_1	9999000000	214450694	141.6bp	128.0bp	0.02%	0.94%	0.39%	0.00%	0.04%	3.21%	0.64%	0.00%	No	0.00%	197018284	0.01%	0.40%	117366840	0.95%	0.97%	0.05%	0.03%	111022776	55991677	5295224	1762931	215.25	93.00	0.67%	0.49%	0.98	0.000				188bp	478bp	40.6%	0.0%	97.3%	91.6%	96.2%	94.8M	26.9%	97.7%	24677.0Mb	197.0M	53.5%	98.5%	56.3%
aih-tih-sc-6e7384-R1_B23WHYVLT4_1_1																																	141.6bp	98.3%	96.8M																88.4%	52%	151bp	151bp	27%	424.3M
aih-tih-sc-6e7384-R1_B23WHYVLT4_1_2																																																			86.6%	53%	151bp	151bp	18%	424.3M
aih-tih-sc-6e7384-R1_B23WHYVLT4_1_trimmed_1																																																			77.8%	53%	128bp	142bp	18%	98.5M	77.8%	53%	128bp	142bp	18%	98.5M
aih-tih-sc-6e7384-R1_B23WHYVLT4_1_trimmed_2																																																			76.1%	53%	128bp	142bp	9%	98.5M	76.1%	53%	128bp	142bp	9%	98.5M
aih-tih-sc-72546a-R1_B23WHYVLT4_1	9999000000	164517330	133.9bp	124.0bp	0.01%	0.93%	0.51%	0.00%	0.03%	3.12%	0.84%	0.00%	No	0.00%	196872808	0.01%	0.43%	112419915	0.94%	0.96%	0.05%	0.04%	105150877	52906831	5872341	2220553	190.76	88.00	0.67%	0.47%	0.98	0.000				153bp	305bp	43.2%	0.0%	96.8%	90.2%	95.8%	94.3M	26.9%	97.6%	24072.3Mb	196.9M	55.3%	98.4%	63.0%
aih-tih-sc-72546a-R1_B23WHYVLT4_1_1																																	133.9bp	97.7%	96.1M																85.9%	54%	151bp	151bp	18%	346.3M
aih-tih-sc-72546a-R1_B23WHYVLT4_1_2																																																			84.3%	55%	151bp	151bp	18%	346.3M
aih-tih-sc-72546a-R1_B23WHYVLT4_1_trimmed_1																																																			74.6%	55%	125bp	132bp	9%	98.4M	74.6%	55%	125bp	132bp	9%	98.4M
aih-tih-sc-72546a-R1_B23WHYVLT4_1_trimmed_2																																																			73.7%	55%	125bp	132bp	9%	98.4M	73.7%	55%	125bp	132bp	9%	98.4M
aih-tih-sc-758b5a-R1_B23WHYVLT4_1	9999000000	302251285	146.0bp	134.0bp	0.03%	0.95%	0.51%	0.00%	0.08%	2.94%	0.76%	0.00%	No	0.00%	196788020	0.01%	0.32%	133112630	0.95%	0.97%	0.05%	0.03%	125822624	63435269	6015778	2015704	248.66	113.00	0.69%	0.52%	0.98	0.000				174bp	445bp	32.6%	0.0%	97.2%	88.8%	95.1%	93.5M	22.4%	97.7%	25109.7Mb	196.8M	53.3%	98.4%	54.0%
aih-tih-sc-758b5a-R1_B23WHYVLT4_1_1																																	146.0bp	98.1%	96.5M																86.9%	52%	151bp	151bp	18%	445.8M
aih-tih-sc-758b5a-R1_B23WHYVLT4_1_2																																																			85.4%	53%	151bp	151bp	18%	445.8M
aih-tih-sc-758b5a-R1_B23WHYVLT4_1_trimmed_1																																																			75.0%	52%	131bp	142bp	9%	98.4M	75.0%	52%	131bp	142bp	9%	98.4M
aih-tih-sc-758b5a-R1_B23WHYVLT4_1_trimmed_2																																																			74.1%	53%	131bp	142bp	9%	98.4M	74.1%	53%	131bp	142bp	9%	98.4M
aih-tih-sc-75c3a9-R1_B23WHYVLT4_1	9999000000	189680107	129.2bp	120.0bp	0.02%	0.92%	0.61%	0.00%	0.04%	2.92%	0.93%	0.00%	No	0.00%	196699622	0.01%	0.42%	114229553	0.94%	0.96%	0.05%	0.04%	107294583	53988029	5703178	2095906	191.61	84.00	0.66%	0.46%	0.98	0.000				152bp	308bp	42.2%	0.0%	96.9%	89.6%	95.5%	93.9M	26.6%	97.6%	23746.4Mb	196.7M	55.8%	98.3%	65.2%
aih-tih-sc-75c3a9-R1_B23WHYVLT4_1_1																																	129.2bp	98.0%	96.4M																88.8%	54%	151bp	151bp	18%	460.3M
aih-tih-sc-75c3a9-R1_B23WHYVLT4_1_2																																																			87.0%	55%	151bp	151bp	18%	460.3M
aih-tih-sc-75c3a9-R1_B23WHYVLT4_1_trimmed_1																																																			77.0%	55%	124bp	127bp	9%	98.3M	77.0%	55%	124bp	127bp	9%	98.3M
aih-tih-sc-75c3a9-R1_B23WHYVLT4_1_trimmed_2																																																			75.8%	56%	124bp	132bp	9%	98.3M	75.8%	56%	124bp	132bp	9%	98.3M
aih-tih-sc-762402-R1_B23WHYVLT4_1	9999000000	216635440	137.1bp	126.0bp	0.01%	0.77%	0.47%	0.00%	0.03%	2.78%	0.82%	0.00%	No	0.00%	196888542	0.01%	0.44%	109407691	0.95%	0.97%	0.04%	0.03%	103454547	52114520	4680213	1552057	197.72	76.00	0.64%	0.45%	0.98	0.000				211bp	503bp	44.7%	0.0%	97.6%	92.2%	96.4%	94.9M	27.6%	97.7%	24416.5Mb	196.9M	54.9%	98.4%	59.2%
aih-tih-sc-762402-R1_B23WHYVLT4_1_1																																	137.1bp	98.3%	96.8M																83.8%	53%	151bp	151bp	27%	215.3M
aih-tih-sc-762402-R1_B23WHYVLT4_1_2																																																			82.0%	55%	151bp	151bp	27%	215.3M
aih-tih-sc-762402-R1_B23WHYVLT4_1_trimmed_1																																																			77.8%	54%	127bp	137bp	18%	98.4M	77.8%	54%	127bp	137bp	18%	98.4M
aih-tih-sc-762402-R1_B23WHYVLT4_1_trimmed_2																																																			76.7%	55%	127bp	137bp	18%	98.4M	76.7%	55%	127bp	137bp	18%	98.4M
aih-tih-sc-76a805-R1_B23WHYVLT4_1	9999000000	229285176	149.3bp	137.0bp	0.02%	1.16%	0.48%	0.00%	0.05%	3.65%	0.77%	0.00%	No	0.00%	196457470	0.01%	0.35%	127308917	0.94%	0.97%	0.05%	0.03%	120067857	60548797	5800208	1910768	235.06	95.00	0.66%	0.49%	0.98	0.000				170bp	350bp	35.5%	0.0%	96.8%	85.2%	93.9%	92.3M	24.7%	97.6%	25116.9Mb	196.5M	56.7%	98.2%	53.0%
aih-tih-sc-76a805-R1_B23WHYVLT4_1_1																																	149.3bp	97.9%	96.2M																88.8%	55%	151bp	151bp	18%	517.0M
aih-tih-sc-76a805-R1_B23WHYVLT4_1_2																																																			87.5%	56%	151bp	151bp	18%	517.0M
aih-tih-sc-76a805-R1_B23WHYVLT4_1_trimmed_1																																																			75.8%	56%	131bp	147bp	9%	98.2M	75.8%	56%	131bp	147bp	9%	98.2M
aih-tih-sc-76a805-R1_B23WHYVLT4_1_trimmed_2																																																			75.4%	57%	131bp	147bp	9%	98.2M	75.4%	57%	131bp	147bp	9%	98.2M
aih-tih-sc-79f487-R1_B23WHYVLT4_1	9999000000	291815729	154.9bp	141.0bp	0.03%	1.43%	0.70%	0.00%	0.07%	4.31%	1.06%	0.00%	No	0.00%	196750352	0.01%	0.33%	132322564	0.95%	0.97%	0.04%	0.03%	125366325	63306296	5663440	1709300	257.74	100.00	0.65%	0.50%	0.98	0.000				198bp	500bp	33.0%	0.0%	97.4%	86.7%	94.2%	92.7M	22.3%	97.6%	25438.3Mb	196.8M	55.3%	98.4%	48.5%
aih-tih-sc-79f487-R1_B23WHYVLT4_1_1																																	154.9bp	98.1%	96.5M																84.1%	54%	151bp	151bp	18%	278.1M
aih-tih-sc-79f487-R1_B23WHYVLT4_1_2																																																			82.4%	55%	151bp	151bp	18%	278.1M
aih-tih-sc-79f487-R1_B23WHYVLT4_1_trimmed_1																																																			75.5%	54%	132bp	151bp	9%	98.4M	75.5%	54%	132bp	151bp	9%	98.4M
aih-tih-sc-79f487-R1_B23WHYVLT4_1_trimmed_2																																																			74.7%	55%	133bp	151bp	9%	98.4M	74.7%	55%	133bp	151bp	9%	98.4M
aih-tih-sc-7c610c-R1_B23WHYVLT4_1	9999000000	168868594	123.5bp	116.0bp	0.07%	1.67%	0.64%	0.00%	0.14%	4.73%	0.95%	0.00%	No	0.00%	196546874	0.01%	0.42%	113871032	0.94%	0.96%	0.05%	0.04%	106747851	53628775	5649086	2165434	179.31	76.00	0.64%	0.45%	0.98	0.000				125bp	212bp	42.4%	0.0%	97.5%	83.0%	92.9%	91.3M	24.3%	97.6%	22843.1Mb	196.5M	54.9%	98.3%	72.7%
aih-tih-sc-7c610c-R1_B23WHYVLT4_1_1																																	123.5bp	98.0%	96.3M																89.4%	53%	151bp	151bp	27%	569.8M
aih-tih-sc-7c610c-R1_B23WHYVLT4_1_2																																																			87.6%	54%	151bp	151bp	18%	569.8M
aih-tih-sc-7c610c-R1_B23WHYVLT4_1_trimmed_1																																																			75.4%	54%	119bp	122bp	18%	98.3M	75.4%	54%	119bp	122bp	18%	98.3M
aih-tih-sc-7c610c-R1_B23WHYVLT4_1_trimmed_2																																																			74.0%	55%	119bp	122bp	9%	98.3M	74.0%	55%	119bp	122bp	9%	98.3M
aih-tih-sc-7c6c07-R1_B23WHYVLT4_1	9999000000	268217269	139.4bp	128.0bp	0.01%	3.03%	0.83%	0.00%	0.02%	8.01%	1.14%	0.00%	No	0.00%	196776510	0.01%	0.36%	126663719	0.94%	0.96%	0.05%	0.04%	119309285	60090090	6202494	2186488	225.91	81.00	0.63%	0.47%	0.98	0.000				156bp	339bp	35.8%	0.0%	97.1%	88.4%	95.0%	93.4M	22.6%	97.6%	24513.6Mb	196.8M	54.2%	98.4%	59.0%
aih-tih-sc-7c6c07-R1_B23WHYVLT4_1_1																																	139.4bp	97.8%	96.2M																85.6%	53%	151bp	151bp	18%	368.7M
aih-tih-sc-7c6c07-R1_B23WHYVLT4_1_2																																																			84.2%	54%	151bp	151bp	18%	368.7M
aih-tih-sc-7c6c07-R1_B23WHYVLT4_1_trimmed_1																																																			75.2%	53%	128bp	137bp	9%	98.4M	75.2%	53%	128bp	137bp	9%	98.4M
aih-tih-sc-7c6c07-R1_B23WHYVLT4_1_trimmed_2																																																			74.4%	54%	128bp	137bp	9%	98.4M	74.4%	54%	128bp	137bp	9%	98.4M
aih-tih-sc-7c8bae-R1_B23WHYVLT4_1	9999000000	304092652	149.4bp	134.0bp	0.03%	1.61%	0.58%	0.00%	0.07%	4.69%	0.87%	0.00%	No	0.00%	196371736	0.00%	0.31%	135093755	0.95%	0.97%	0.04%	0.03%	128146309	64662308	5982965	1846687	255.30	92.00	0.63%	0.49%	0.98	0.000				206bp	620bp	31.4%	0.0%	97.4%	90.8%	96.0%	94.2M	21.4%	97.7%	24865.5Mb	196.4M	52.0%	98.2%	52.4%
aih-tih-sc-7c8bae-R1_B23WHYVLT4_1_1																																	149.4bp	98.2%	96.4M																86.0%	51%	151bp	151bp	18%	312.9M
aih-tih-sc-7c8bae-R1_B23WHYVLT4_1_2																																																			84.3%	52%	151bp	151bp	18%	312.9M
aih-tih-sc-7c8bae-R1_B23WHYVLT4_1_trimmed_1																																																			77.0%	51%	129bp	147bp	9%	98.2M	77.0%	51%	129bp	147bp	9%	98.2M
aih-tih-sc-7c8bae-R1_B23WHYVLT4_1_trimmed_2																																																			76.3%	52%	130bp	147bp	9%	98.2M	76.3%	52%	130bp	147bp	9%	98.2M
aih-tih-sc-7d62c4-R1_B23WHYVLT4_1	9999000000	222316855	147.7bp	136.0bp	0.01%	0.79%	0.50%	0.00%	0.03%	2.48%	0.75%	0.00%	No	0.00%	196746334	0.01%	0.36%	125403456	0.94%	0.97%	0.05%	0.03%	118106294	59559611	5910753	1971125	230.58	111.00	0.70%	0.52%	0.98	0.000				179bp	412bp	36.5%	0.0%	96.8%	89.5%	95.5%	93.9M	25.6%	97.5%	25263.5Mb	196.7M	54.9%	98.4%	53.1%
aih-tih-sc-7d62c4-R1_B23WHYVLT4_1_1																																	147.7bp	97.9%	96.3M																86.9%	54%	151bp	151bp	18%	388.3M
aih-tih-sc-7d62c4-R1_B23WHYVLT4_1_2																																																			85.2%	54%	151bp	151bp	18%	388.3M
aih-tih-sc-7d62c4-R1_B23WHYVLT4_1_trimmed_1																																																			75.1%	54%	132bp	147bp	9%	98.4M	75.1%	54%	132bp	147bp	9%	98.4M
aih-tih-sc-7d62c4-R1_B23WHYVLT4_1_trimmed_2																																																			74.0%	55%	132bp	147bp	9%	98.4M	74.0%	55%	132bp	147bp	9%	98.4M
aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1	9999000000	101344272	131.6bp	123.0bp	0.02%	0.72%	0.48%	0.00%	0.04%	2.18%	0.70%	0.00%	No	0.00%	196153888	0.01%	0.52%	93876812	0.93%	0.96%	0.05%	0.04%	87189731	43815408	5081431	1936663	151.34	60.00	0.63%	0.38%	0.98	0.000				138bp	241bp	52.6%	0.0%	97.2%	87.6%	94.8%	92.9M	34.4%	97.6%	23681.1Mb	196.2M	58.0%	98.1%	66.6%
aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1_1																																	131.6bp	97.5%	95.6M																86.1%	56%	151bp	151bp	27%	213.8M
aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1_2																																																			84.1%	57%	151bp	151bp	18%	213.8M
aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1_trimmed_1																																																			79.3%	57%	124bp	127bp	18%	98.1M	79.3%	57%	124bp	127bp	18%	98.1M
aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1_trimmed_2																																																			78.3%	58%	124bp	127bp	9%	98.1M	78.3%	58%	124bp	127bp	9%	98.1M
aih-tih-sc-7fc50e-R1_B23WHYVLT4_1	9999000000	133641696	130.1bp	121.0bp	0.02%	0.81%	0.36%	0.00%	0.04%	2.49%	0.54%	0.00%	No	0.00%	196834078	0.01%	0.46%	106689001	0.92%	0.95%	0.07%	0.05%	98235815	49402667	6935662	2802258	170.82	77.00	0.65%	0.44%	0.98	0.000				135bp	234bp	46.2%	0.0%	96.2%	88.1%	95.0%	93.5M	29.3%	97.8%	23658.9Mb	196.8M	53.3%	98.4%	67.0%
aih-tih-sc-7fc50e-R1_B23WHYVLT4_1_1																																	130.1bp	97.2%	95.6M																82.2%	52%	151bp	151bp	18%	183.9M
aih-tih-sc-7fc50e-R1_B23WHYVLT4_1_2																																																			80.3%	53%	151bp	151bp	18%	183.9M
aih-tih-sc-7fc50e-R1_B23WHYVLT4_1_trimmed_1																																																			76.1%	53%	123bp	127bp	9%	98.4M	76.1%	53%	123bp	127bp	9%	98.4M
aih-tih-sc-7fc50e-R1_B23WHYVLT4_1_trimmed_2																																																			74.9%	53%	123bp	127bp	9%	98.4M	74.9%	53%	123bp	127bp	9%	98.4M
aih-tih-sc-82e8c1-R1_B23WHYVLT4_1	9999000000	250957540	134.8bp	124.0bp	0.03%	1.43%	0.49%	0.00%	0.07%	4.37%	0.71%	0.00%	No	0.00%	197041088	0.01%	0.34%	129227608	0.94%	0.96%	0.05%	0.04%	121251426	61043793	6926520	2614553	222.17	96.00	0.67%	0.49%	0.98	0.000				141bp	294bp	34.6%	0.0%	96.8%	90.3%	95.5%	94.1M	22.3%	97.8%	24115.7Mb	197.0M	50.7%	98.5%	62.4%
aih-tih-sc-82e8c1-R1_B23WHYVLT4_1_1																																	134.8bp	97.6%	96.2M																83.8%	50%	151bp	151bp	27%	286.6M
aih-tih-sc-82e8c1-R1_B23WHYVLT4_1_2																																																			82.1%	51%	151bp	151bp	18%	286.6M
aih-tih-sc-82e8c1-R1_B23WHYVLT4_1_trimmed_1																																																			75.0%	50%	125bp	132bp	18%	98.5M	75.0%	50%	125bp	132bp	18%	98.5M
aih-tih-sc-82e8c1-R1_B23WHYVLT4_1_trimmed_2																																																			73.6%	51%	125bp	132bp	9%	98.5M	73.6%	51%	125bp	132bp	9%	98.5M
aih-tih-sc-884b4c-R1_B23WHYVLT4_1	9999000000	121137182	139.7bp	129.0bp	0.00%	0.38%	0.26%	0.00%	0.01%	1.36%	0.46%	0.00%	No	0.00%	196112288	0.01%	0.49%	99516491	0.93%	0.96%	0.06%	0.04%	92320132	46468776	5506158	2023002	172.19	79.00	0.66%	0.45%	0.98	0.000				172bp	335bp	49.7%	0.0%	96.6%	87.8%	95.0%	93.2M	33.7%	97.5%	24467.7Mb	196.1M	57.4%	98.1%	58.3%
aih-tih-sc-884b4c-R1_B23WHYVLT4_1_1																																	139.7bp	97.3%	95.4M																89.9%	56%	151bp	151bp	27%	441.4M
aih-tih-sc-884b4c-R1_B23WHYVLT4_1_2																																																			87.9%	57%	151bp	151bp	27%	441.4M
aih-tih-sc-884b4c-R1_B23WHYVLT4_1_trimmed_1																																																			77.5%	57%	128bp	137bp	18%	98.1M	77.5%	57%	128bp	137bp	18%	98.1M
aih-tih-sc-884b4c-R1_B23WHYVLT4_1_trimmed_2																																																			76.6%	57%	128bp	137bp	18%	98.1M	76.6%	57%	128bp	137bp	18%	98.1M
aih-tih-sc-89cc59-R1_B23WHYVLT4_1	9999000000	47288001	119.3bp	113.0bp	0.00%	0.52%	0.41%	0.00%	0.01%	1.69%	0.76%	0.00%	No	0.00%	194886180	0.01%	0.76%	47576482	0.85%	0.92%	0.09%	0.08%	40654353	20352772	4098835	1725697	60.58	8.00	0.32%	0.15%	0.97	0.000				129bp	199bp	76.7%	0.0%	96.6%	81.6%	92.4%	90.1M	48.3%	97.1%	22175.3Mb	194.9M	64.3%	97.4%	75.7%
aih-tih-sc-89cc59-R1_B23WHYVLT4_1_1																																	119.3bp	96.8%	94.3M																90.4%	60%	151bp	151bp	27%	181.0M
aih-tih-sc-89cc59-R1_B23WHYVLT4_1_2																																																			87.9%	62%	151bp	151bp	36%	181.0M
aih-tih-sc-89cc59-R1_B23WHYVLT4_1_trimmed_1																																																			86.5%	64%	117bp	117bp	18%	97.4M	86.5%	64%	117bp	117bp	18%	97.4M
aih-tih-sc-89cc59-R1_B23WHYVLT4_1_trimmed_2																																																			85.7%	64%	117bp	117bp	9%	97.4M	85.7%	64%	117bp	117bp	9%	97.4M
aih-tih-sc-8a623b-R1_B23WHYVLT4_1	9999000000	175525286	125.0bp	116.0bp	0.02%	0.91%	0.53%	0.00%	0.04%	2.85%	0.81%	0.00%	No	0.00%	196753320	0.01%	0.44%	109890449	0.94%	0.96%	0.05%	0.04%	102752063	51650907	5824274	2252975	175.45	79.00	0.66%	0.45%	0.98	0.000				137bp	274bp	44.4%	0.0%	96.8%	90.2%	95.8%	94.2M	24.7%	97.7%	23107.9Mb	196.8M	53.9%	98.4%	70.8%
aih-tih-sc-8a623b-R1_B23WHYVLT4_1_1																																	125.0bp	97.7%	96.1M																84.1%	52%	151bp	151bp	18%	314.8M
aih-tih-sc-8a623b-R1_B23WHYVLT4_1_2																																																			82.2%	53%	151bp	151bp	18%	314.8M
aih-tih-sc-8a623b-R1_B23WHYVLT4_1_trimmed_1																																																			73.5%	53%	120bp	122bp	9%	98.4M	73.5%	53%	120bp	122bp	9%	98.4M
aih-tih-sc-8a623b-R1_B23WHYVLT4_1_trimmed_2																																																			72.3%	54%	120bp	122bp	9%	98.4M	72.3%	54%	120bp	122bp	9%	98.4M
aih-tih-sc-8b4971-R1_B23WHYVLT4_1	9999000000	186317646	134.4bp	125.0bp	0.02%	0.75%	0.47%	0.00%	0.05%	2.41%	0.72%	0.00%	No	0.00%	196843592	0.01%	0.40%	118714039	0.93%	0.96%	0.05%	0.04%	110984198	55855421	6422362	2437464	197.67	89.00	0.66%	0.48%	0.98	0.000				140bp	249bp	40.0%	0.0%	96.4%	87.3%	95.0%	93.5M	24.4%	97.7%	24035.9Mb	196.8M	53.7%	98.4%	64.9%
aih-tih-sc-8b4971-R1_B23WHYVLT4_1_1																																	134.4bp	97.5%	96.0M																83.0%	52%	151bp	151bp	18%	291.1M
aih-tih-sc-8b4971-R1_B23WHYVLT4_1_2																																																			81.2%	53%	151bp	151bp	18%	291.1M
aih-tih-sc-8b4971-R1_B23WHYVLT4_1_trimmed_1																																																			72.5%	53%	125bp	132bp	9%	98.4M	72.5%	53%	125bp	132bp	9%	98.4M
aih-tih-sc-8b4971-R1_B23WHYVLT4_1_trimmed_2																																																			71.2%	53%	125bp	132bp	9%	98.4M	71.2%	53%	125bp	132bp	9%	98.4M
aih-tih-sc-8b9375-R1_B23WHYVLT4_1	9999000000	309302197	144.5bp	132.0bp	0.04%	0.76%	0.41%	0.00%	0.10%	2.32%	0.65%	0.00%	No	0.00%	196451008	0.01%	0.32%	133989608	0.94%	0.96%	0.05%	0.04%	125324613	63248221	7070230	2449866	241.74	110.00	0.68%	0.52%	0.98	0.000				166bp	391bp	32.1%	0.0%	96.3%	89.6%	95.4%	93.7M	21.5%	97.8%	24693.9Mb	196.5M	52.1%	98.2%	55.1%
aih-tih-sc-8b9375-R1_B23WHYVLT4_1_1																																	144.5bp	97.5%	95.8M																86.2%	51%	151bp	151bp	18%	396.4M
aih-tih-sc-8b9375-R1_B23WHYVLT4_1_2																																																			84.4%	52%	151bp	151bp	18%	396.4M
aih-tih-sc-8b9375-R1_B23WHYVLT4_1_trimmed_1																																																			75.1%	51%	128bp	142bp	9%	98.2M	75.1%	51%	128bp	142bp	9%	98.2M
aih-tih-sc-8b9375-R1_B23WHYVLT4_1_trimmed_2																																																			74.0%	52%	129bp	142bp	9%	98.2M	74.0%	52%	129bp	142bp	9%	98.2M
aih-tih-sc-8d3f7c-R1_B23WHYVLT4_1	9999000000	113275465	115.9bp	109.0bp	0.06%	0.69%	0.40%	0.00%	0.12%	2.02%	0.57%	0.00%	No	0.00%	196849686	0.01%	0.54%	89675910	0.91%	0.93%	0.08%	0.07%	81350239	40820305	6997533	3031270	123.87	51.00	0.59%	0.36%	0.98	0.000				115bp	168bp	54.8%	0.0%	96.2%	88.0%	95.1%	93.6M	33.1%	97.8%	22015.3Mb	196.8M	52.3%	98.4%	79.2%
aih-tih-sc-8d3f7c-R1_B23WHYVLT4_1_1																																	115.9bp	97.1%	95.6M																84.0%	51%	151bp	151bp	18%	204.9M
aih-tih-sc-8d3f7c-R1_B23WHYVLT4_1_2																																																			82.1%	53%	151bp	151bp	18%	204.9M
aih-tih-sc-8d3f7c-R1_B23WHYVLT4_1_trimmed_1																																																			76.9%	52%	114bp	112bp	9%	98.4M	76.9%	52%	114bp	112bp	9%	98.4M
aih-tih-sc-8d3f7c-R1_B23WHYVLT4_1_trimmed_2																																																			75.6%	52%	114bp	112bp	9%	98.4M	75.6%	52%	114bp	112bp	9%	98.4M
aih-tih-sc-910896-R1_B23WHYVLT4_1	9999000000	118429467	138.5bp	128.0bp	0.03%	1.06%	0.49%	0.00%	0.07%	3.25%	0.74%	0.00%	No	0.00%	196558752	0.01%	0.48%	101705910	0.93%	0.95%	0.06%	0.05%	94220303	47423139	6197487	2383374	170.97	74.00	0.64%	0.44%	0.98	0.000				146bp	264bp	48.6%	0.0%	96.6%	88.2%	95.2%	93.6M	33.3%	97.6%	24355.0Mb	196.6M	55.4%	98.3%	61.0%
aih-tih-sc-910896-R1_B23WHYVLT4_1_1																																	138.5bp	97.5%	95.8M																85.9%	54%	151bp	151bp	18%	245.0M
aih-tih-sc-910896-R1_B23WHYVLT4_1_2																																																			84.1%	55%	151bp	151bp	18%	245.0M
aih-tih-sc-910896-R1_B23WHYVLT4_1_trimmed_1																																																			77.5%	55%	127bp	137bp	9%	98.3M	77.5%	55%	127bp	137bp	9%	98.3M
aih-tih-sc-910896-R1_B23WHYVLT4_1_trimmed_2																																																			76.7%	55%	127bp	137bp	9%	98.3M	76.7%	55%	127bp	137bp	9%	98.3M
aih-tih-sc-9114fc-R1_B23WHYVLT4_1	9999000000	270601032	157.7bp	143.0bp	0.02%	1.29%	0.49%	0.00%	0.04%	4.04%	0.78%	0.00%	No	0.00%	196758014	0.01%	0.32%	133042092	0.94%	0.97%	0.05%	0.03%	125456376	63418657	6426100	2043650	258.06	123.00	0.68%	0.54%	0.98	0.000				204bp	489bp	32.6%	0.0%	96.5%	89.9%	95.8%	94.3M	24.1%	97.7%	25823.4Mb	196.8M	55.1%	98.4%	46.2%
aih-tih-sc-9114fc-R1_B23WHYVLT4_1_1																																	157.7bp	97.8%	96.2M																87.6%	54%	151bp	151bp	18%	457.9M
aih-tih-sc-9114fc-R1_B23WHYVLT4_1_2																																																			86.1%	55%	151bp	151bp	18%	457.9M
aih-tih-sc-9114fc-R1_B23WHYVLT4_1_trimmed_1																																																			75.4%	54%	134bp	151bp	9%	98.4M	75.4%	54%	134bp	151bp	9%	98.4M
aih-tih-sc-9114fc-R1_B23WHYVLT4_1_trimmed_2																																																			74.4%	55%	134bp	151bp	9%	98.4M	74.4%	55%	134bp	151bp	9%	98.4M
aih-tih-sc-934f55-R1_B23WHYVLT4_1	9999000000	308917534	143.7bp	132.0bp	0.03%	0.85%	0.40%	0.00%	0.06%	2.69%	0.61%	0.00%	No	0.00%	196758878	0.01%	0.31%	134820310	0.94%	0.97%	0.05%	0.03%	126831332	63953518	6071998	2031785	245.30	116.00	0.69%	0.53%	0.98	0.000				160bp	349bp	31.8%	0.0%	97.0%	87.0%	94.4%	92.8M	19.8%	97.6%	24706.4Mb	196.8M	53.9%	98.4%	56.3%
aih-tih-sc-934f55-R1_B23WHYVLT4_1_1																																	143.7bp	98.0%	96.4M																84.3%	53%	151bp	151bp	27%	369.1M
aih-tih-sc-934f55-R1_B23WHYVLT4_1_2																																																			82.6%	53%	151bp	151bp	18%	369.1M
aih-tih-sc-934f55-R1_B23WHYVLT4_1_trimmed_1																																																			72.8%	53%	129bp	142bp	9%	98.4M	72.8%	53%	129bp	142bp	9%	98.4M
aih-tih-sc-934f55-R1_B23WHYVLT4_1_trimmed_2																																																			71.8%	54%	129bp	142bp	9%	98.4M	71.8%	54%	129bp	142bp	9%	98.4M
aih-tih-sc-973c73-R1_B23WHYVLT4_1	9999000000	228484068	128.3bp	119.0bp	0.02%	0.77%	0.45%	0.00%	0.05%	2.46%	0.70%	0.00%	No	0.00%	197057530	0.01%	0.37%	124559023	0.94%	0.96%	0.05%	0.04%	116919497	58814283	6387112	2434337	204.69	88.00	0.67%	0.47%	0.98	0.000				140bp	278bp	37.0%	0.0%	96.7%	89.8%	95.7%	94.3M	22.7%	97.7%	23508.1Mb	197.1M	54.2%	98.5%	67.4%
aih-tih-sc-973c73-R1_B23WHYVLT4_1_1																																	128.3bp	97.9%	96.4M																87.8%	53%	151bp	151bp	18%	448.3M
aih-tih-sc-973c73-R1_B23WHYVLT4_1_2																																																			86.3%	54%	151bp	151bp	18%	448.3M
aih-tih-sc-973c73-R1_B23WHYVLT4_1_trimmed_1																																																			75.9%	53%	122bp	127bp	9%	98.5M	75.9%	53%	122bp	127bp	9%	98.5M
aih-tih-sc-973c73-R1_B23WHYVLT4_1_trimmed_2																																																			74.7%	54%	122bp	127bp	9%	98.5M	74.7%	54%	122bp	127bp	9%	98.5M
aih-tih-sc-976eb1-R1_B23WHYVLT4_1	9999000000	38671956	115.3bp	110.0bp	0.03%	0.55%	0.25%	0.00%	0.06%	1.74%	0.40%	0.00%	No	0.00%	144234166	0.03%	0.76%	35145874	0.72%	0.84%	0.15%	0.16%	25329581	12670177	5221586	2430726	36.14	14.00	0.34%	0.08%	0.98	0.000				112bp	141bp	78.1%	0.0%	95.1%	82.8%	91.8%	66.2M	45.5%	97.6%	15975.2Mb	144.2M	54.4%	97.5%	80.2%
aih-tih-sc-976eb1-R1_B23WHYVLT4_1_1																																	115.3bp	94.0%	67.8M																83.3%	53%	151bp	151bp	27%	74.0M
aih-tih-sc-976eb1-R1_B23WHYVLT4_1_2																																																			81.0%	54%	151bp	151bp	27%	74.0M
aih-tih-sc-976eb1-R1_B23WHYVLT4_1_trimmed_1																																																			83.1%	54%	113bp	112bp	18%	72.1M	83.1%	54%	113bp	112bp	18%	72.1M
aih-tih-sc-976eb1-R1_B23WHYVLT4_1_trimmed_2																																																			82.2%	54%	114bp	112bp	9%	72.1M	82.2%	54%	114bp	112bp	9%	72.1M
aih-tih-sc-97c26d-R1_B23WHYVLT4_1	9999000000	76932570	106.7bp	102.0bp	0.04%	1.52%	0.42%	0.00%	0.08%	4.41%	0.63%	0.00%	No	0.00%	196619568	0.01%	0.68%	61958390	0.86%	0.89%	0.12%	0.11%	53195037	26647848	7320601	3359627	73.14	26.00	0.48%	0.21%	0.98	0.000				104bp	141bp	69.0%	0.0%	95.7%	88.1%	95.1%	93.5M	37.4%	97.7%	20581.3Mb	196.6M	51.4%	98.3%	86.1%
aih-tih-sc-97c26d-R1_B23WHYVLT4_1_1																																	106.7bp	96.4%	94.8M																81.1%	51%	151bp	151bp	18%	112.3M
aih-tih-sc-97c26d-R1_B23WHYVLT4_1_2																																																			79.2%	51%	151bp	151bp	27%	112.3M
aih-tih-sc-97c26d-R1_B23WHYVLT4_1_trimmed_1																																																			79.8%	51%	107bp	107bp	18%	98.3M	79.8%	51%	107bp	107bp	18%	98.3M
aih-tih-sc-97c26d-R1_B23WHYVLT4_1_trimmed_2																																																			78.7%	51%	107bp	107bp	9%	98.3M	78.7%	51%	107bp	107bp	9%	98.3M
aih-tih-sc-97ecde-R1_B23WHYVLT4_1	9999000000	62947141	123.8bp	115.0bp	0.04%	0.64%	0.30%	0.00%	0.08%	2.08%	0.48%	0.00%	Yes	24.06%	196435664	0.01%	0.62%	73931534	0.88%	0.92%	0.09%	0.08%	65345251	32817574	6786182	2941062	105.36	48.00	0.59%	0.32%	0.98	0.000				123bp	186bp	62.9%	0.0%	95.8%	86.5%	94.6%	92.9M	38.6%	97.7%	22714.6Mb	196.4M	54.8%	98.2%	72.2%
aih-tih-sc-97ecde-R1_B23WHYVLT4_1_1																																	123.8bp	96.9%	95.2M																87.6%	53%	151bp	151bp	27%	254.3M
aih-tih-sc-97ecde-R1_B23WHYVLT4_1_2																																																			85.5%	55%	151bp	151bp	18%	254.3M
aih-tih-sc-97ecde-R1_B23WHYVLT4_1_trimmed_1																																																			79.2%	54%	118bp	122bp	18%	98.2M	79.2%	54%	118bp	122bp	18%	98.2M
aih-tih-sc-97ecde-R1_B23WHYVLT4_1_trimmed_2																																																			77.6%	55%	119bp	122bp	9%	98.2M	77.6%	55%	119bp	122bp	9%	98.2M
aih-tih-sc-a03f86-R1_B23WHYVLT4_1	9999000000	254671443	150.3bp	136.0bp	0.03%	0.81%	0.48%	0.00%	0.08%	2.76%	0.77%	0.00%	No	0.00%	197018374	0.01%	0.37%	124269732	0.94%	0.97%	0.05%	0.03%	116840907	59005516	6240971	2094487	233.52	108.00	0.69%	0.52%	0.98	0.000				199bp	497bp	37.1%	0.0%	96.7%	90.7%	96.0%	94.6M	25.4%	97.7%	25191.0Mb	197.0M	53.8%	98.5%	51.8%
aih-tih-sc-a03f86-R1_B23WHYVLT4_1_1																																	150.3bp	97.9%	96.4M																81.3%	53%	151bp	151bp	18%	161.5M
aih-tih-sc-a03f86-R1_B23WHYVLT4_1_2																																																			78.6%	54%	151bp	151bp	18%	161.5M
aih-tih-sc-a03f86-R1_B23WHYVLT4_1_trimmed_1																																																			77.1%	53%	131bp	147bp	9%	98.5M	77.1%	53%	131bp	147bp	9%	98.5M
aih-tih-sc-a03f86-R1_B23WHYVLT4_1_trimmed_2																																																			75.0%	54%	131bp	147bp	9%	98.5M	75.0%	54%	131bp	147bp	9%	98.5M
aih-tih-sc-a3a521-R1_B23WHYVLT4_1	9999000000	249846611	136.8bp	126.0bp	0.04%	0.90%	0.64%	0.00%	0.08%	2.67%	0.91%	0.00%	No	0.00%	197011026	0.01%	0.34%	130473770	0.94%	0.96%	0.05%	0.04%	122883733	61901820	6368228	2295538	226.19	110.00	0.68%	0.52%	0.98	0.000				153bp	365bp	34.0%	0.0%	97.0%	90.3%	95.7%	94.3M	22.1%	97.8%	24182.6Mb	197.0M	52.2%	98.5%	61.6%
aih-tih-sc-a3a521-R1_B23WHYVLT4_1_1																																	136.8bp	97.8%	96.4M																84.1%	51%	151bp	151bp	18%	299.5M
aih-tih-sc-a3a521-R1_B23WHYVLT4_1_2																																																			82.7%	52%	151bp	151bp	18%	299.5M
aih-tih-sc-a3a521-R1_B23WHYVLT4_1_trimmed_1																																																			74.5%	51%	125bp	132bp	9%	98.5M	74.5%	51%	125bp	132bp	9%	98.5M
aih-tih-sc-a3a521-R1_B23WHYVLT4_1_trimmed_2																																																			73.4%	52%	126bp	132bp	9%	98.5M	73.4%	52%	126bp	132bp	9%	98.5M
aih-tih-sc-a49d10-R1_B23WHYVLT4_1	9999000000	223964576	133.1bp	124.0bp	0.05%	0.91%	0.38%	0.00%	0.11%	2.79%	0.54%	0.00%	No	0.00%	196770766	0.01%	0.37%	124183802	0.94%	0.96%	0.05%	0.04%	116122283	58405688	6282826	2364384	209.27	82.00	0.63%	0.47%	0.98	0.000				141bp	291bp	37.2%	0.0%	97.2%	87.0%	94.6%	93.1M	24.1%	97.6%	23954.3Mb	196.8M	53.2%	98.4%	64.8%
aih-tih-sc-a49d10-R1_B23WHYVLT4_1_1																																	133.1bp	98.0%	96.4M																88.3%	52%	151bp	151bp	27%	476.7M
aih-tih-sc-a49d10-R1_B23WHYVLT4_1_2																																																			86.3%	53%	151bp	151bp	18%	476.7M
aih-tih-sc-a49d10-R1_B23WHYVLT4_1_trimmed_1																																																			76.0%	52%	125bp	132bp	18%	98.4M	76.0%	52%	125bp	132bp	18%	98.4M
aih-tih-sc-a49d10-R1_B23WHYVLT4_1_trimmed_2																																																			74.5%	53%	125bp	132bp	9%	98.4M	74.5%	53%	125bp	132bp	9%	98.4M
aih-tih-sc-a70f1e-R1_B23WHYVLT4_1	9999000000	69927622	113.0bp	107.0bp	0.03%	1.98%	1.03%	0.00%	0.06%	5.25%	1.38%	0.00%	No	0.00%	196516214	0.01%	0.63%	73636898	0.89%	0.92%	0.09%	0.08%	65875815	33035171	6335467	2778125	97.24	31.00	0.51%	0.29%	0.98	0.000				111bp	167bp	63.0%	0.0%	96.5%	87.9%	94.9%	93.3M	34.8%	97.8%	21463.8Mb	196.5M	51.6%	98.3%	81.8%
aih-tih-sc-a70f1e-R1_B23WHYVLT4_1_1																																	113.0bp	97.1%	95.4M																87.7%	51%	151bp	151bp	18%	250.4M
aih-tih-sc-a70f1e-R1_B23WHYVLT4_1_2																																																			86.1%	52%	151bp	151bp	27%	250.4M
aih-tih-sc-a70f1e-R1_B23WHYVLT4_1_trimmed_1																																																			79.5%	51%	112bp	112bp	9%	98.3M	79.5%	51%	112bp	112bp	9%	98.3M
aih-tih-sc-a70f1e-R1_B23WHYVLT4_1_trimmed_2																																																			78.6%	51%	112bp	112bp	9%	98.3M	78.6%	51%	112bp	112bp	9%	98.3M
aih-tih-sc-ac6a71-R1_B23WHYVLT4_1	9999000000	253400109	147.1bp	134.0bp	0.03%	1.08%	0.59%	0.00%	0.07%	3.50%	0.90%	0.00%	No	0.00%	197031828	0.01%	0.36%	125220907	0.95%	0.97%	0.04%	0.03%	118505420	59816113	5633782	1810075	234.78	103.00	0.68%	0.51%	0.98	0.000				205bp	531bp	36.6%	0.0%	97.1%	91.9%	96.4%	95.0M	25.2%	97.7%	25038.4Mb	197.0M	53.7%	98.5%	53.0%
aih-tih-sc-ac6a71-R1_B23WHYVLT4_1_1																																	147.1bp	98.2%	96.7M																86.7%	52%	151bp	151bp	18%	334.4M
aih-tih-sc-ac6a71-R1_B23WHYVLT4_1_2																																																			84.9%	53%	151bp	151bp	18%	334.4M
aih-tih-sc-ac6a71-R1_B23WHYVLT4_1_trimmed_1																																																			77.4%	53%	130bp	147bp	9%	98.5M	77.4%	53%	130bp	147bp	9%	98.5M
aih-tih-sc-ac6a71-R1_B23WHYVLT4_1_trimmed_2																																																			75.8%	54%	130bp	147bp	9%	98.5M	75.8%	54%	130bp	147bp	9%	98.5M
aih-tih-sc-b157e9-R1_B23WHYVLT4_1	9999000000	172873164	134.4bp	124.0bp	0.03%	0.79%	0.43%	0.00%	0.07%	2.34%	0.62%	0.00%	No	0.00%	196676680	0.01%	0.42%	114703229	0.94%	0.97%	0.05%	0.03%	107277500	53956811	5189329	1870626	193.11	85.00	0.66%	0.47%	0.98	0.000				142bp	271bp	42.2%	0.0%	97.3%	87.2%	94.2%	92.6M	25.8%	97.7%	23974.2Mb	196.7M	54.9%	98.3%	64.6%
aih-tih-sc-b157e9-R1_B23WHYVLT4_1_1																																	134.4bp	98.0%	96.3M																86.8%	54%	151bp	151bp	27%	393.8M
aih-tih-sc-b157e9-R1_B23WHYVLT4_1_2																																																			85.0%	55%	151bp	151bp	18%	393.8M
aih-tih-sc-b157e9-R1_B23WHYVLT4_1_trimmed_1																																																			74.7%	54%	125bp	132bp	18%	98.3M	74.7%	54%	125bp	132bp	18%	98.3M
aih-tih-sc-b157e9-R1_B23WHYVLT4_1_trimmed_2																																																			73.4%	55%	125bp	132bp	9%	98.3M	73.4%	55%	125bp	132bp	9%	98.3M
aih-tih-sc-b845f7-R1_B23WHYVLT4_1	9999000000	205877233	140.5bp	130.0bp	0.03%	0.83%	0.34%	0.00%	0.06%	2.53%	0.52%	0.00%	Yes	0.92%	196617850	0.01%	0.38%	122814891	0.93%	0.96%	0.06%	0.04%	114427928	57699198	6867595	2510769	212.21	95.00	0.68%	0.49%	0.98	0.000				151bp	278bp	37.8%	0.0%	95.8%	88.4%	95.1%	93.5M	25.5%	97.6%	24607.0Mb	196.6M	55.8%	98.3%	58.7%
aih-tih-sc-b845f7-R1_B23WHYVLT4_1_1																																	140.5bp	97.4%	95.7M																85.0%	54%	151bp	151bp	27%	286.1M
aih-tih-sc-b845f7-R1_B23WHYVLT4_1_2																																																			83.1%	55%	151bp	151bp	18%	286.1M
aih-tih-sc-b845f7-R1_B23WHYVLT4_1_trimmed_1																																																			75.6%	55%	128bp	137bp	18%	98.3M	75.6%	55%	128bp	137bp	18%	98.3M
aih-tih-sc-b845f7-R1_B23WHYVLT4_1_trimmed_2																																																			74.4%	56%	128bp	137bp	9%	98.3M	74.4%	56%	128bp	137bp	9%	98.3M
aih-tih-sc-b93682-R1_B23WHYVLT4_1	9999000000	272635907	169.9bp	154.0bp	0.04%	2.61%	0.60%	0.00%	0.07%	7.74%	0.93%	0.00%	No	0.00%	196965852	0.01%	0.37%	123183629	0.94%	0.97%	0.05%	0.03%	116369745	58982048	5822341	1630239	254.03	85.00	0.63%	0.48%	0.98	0.000				304bp	877bp	37.6%	0.0%	97.1%	91.1%	96.3%	94.9M	28.0%	97.7%	26307.7Mb	197.0M	53.8%	98.5%	40.5%
aih-tih-sc-b93682-R1_B23WHYVLT4_1_1																																	169.9bp	97.9%	96.5M																88.5%	53%	151bp	151bp	18%	440.3M
aih-tih-sc-b93682-R1_B23WHYVLT4_1_2																																																			87.0%	54%	151bp	151bp	18%	440.3M
aih-tih-sc-b93682-R1_B23WHYVLT4_1_trimmed_1																																																			78.6%	53%	137bp	151bp	9%	98.5M	78.6%	53%	137bp	151bp	9%	98.5M
aih-tih-sc-b93682-R1_B23WHYVLT4_1_trimmed_2																																																			77.5%	54%	137bp	151bp	9%	98.5M	77.5%	54%	137bp	151bp	9%	98.5M
aih-tih-sc-be4b28-R1_B23WHYVLT4_1	9999000000	158484800	123.2bp	115.0bp	0.04%	1.28%	0.53%	0.00%	0.09%	3.51%	0.74%	0.00%	No	0.00%	196711426	0.01%	0.44%	110919849	0.94%	0.96%	0.05%	0.04%	104244687	52371423	5518213	2123725	173.58	70.00	0.62%	0.43%	0.98	0.000				127bp	250bp	43.9%	0.0%	97.4%	90.0%	95.6%	94.0M	25.6%	97.8%	22827.6Mb	196.7M	52.1%	98.4%	73.2%
aih-tih-sc-be4b28-R1_B23WHYVLT4_1_1																																	123.2bp	98.0%	96.4M																83.8%	51%	151bp	151bp	27%	230.0M
aih-tih-sc-be4b28-R1_B23WHYVLT4_1_2																																																			81.9%	52%	151bp	151bp	27%	230.0M
aih-tih-sc-be4b28-R1_B23WHYVLT4_1_trimmed_1																																																			76.1%	51%	119bp	122bp	18%	98.4M	76.1%	51%	119bp	122bp	18%	98.4M
aih-tih-sc-be4b28-R1_B23WHYVLT4_1_trimmed_2																																																			74.9%	52%	119bp	122bp	9%	98.4M	74.9%	52%	119bp	122bp	9%	98.4M
aih-tih-sc-c04072-R1_B23WHYVLT4_1	9999000000	171175814	148.4bp	139.0bp	0.01%	0.64%	0.51%	0.00%	0.03%	2.25%	0.84%	0.00%	No	0.00%	197018056	0.01%	0.45%	108145727	0.94%	0.97%	0.05%	0.03%	101793973	51363015	4977517	1645232	202.40	88.00	0.66%	0.47%	0.98	0.000				222bp	547bp	45.4%	0.0%	96.9%	91.3%	95.9%	94.5M	32.1%	97.7%	25629.6Mb	197.0M	54.9%	98.5%	51.5%
aih-tih-sc-c04072-R1_B23WHYVLT4_1_1																																	148.4bp	98.0%	96.5M																87.0%	53%	151bp	151bp	18%	314.9M
aih-tih-sc-c04072-R1_B23WHYVLT4_1_2																																																			85.4%	54%	151bp	151bp	18%	314.9M
aih-tih-sc-c04072-R1_B23WHYVLT4_1_trimmed_1																																																			77.6%	54%	133bp	147bp	18%	98.5M	77.6%	54%	133bp	147bp	18%	98.5M
aih-tih-sc-c04072-R1_B23WHYVLT4_1_trimmed_2																																																			76.9%	55%	133bp	147bp	18%	98.5M	76.9%	55%	133bp	147bp	18%	98.5M
aih-tih-sc-c55577-R1_B23WHYVLT4_1	9999000000	53197336	132.5bp	124.0bp	0.05%	1.10%	0.43%	0.00%	0.12%	3.59%	0.74%	0.00%	No	0.00%	195585534	0.01%	0.67%	64343095	0.90%	0.94%	0.07%	0.06%	57648291	28953399	4504277	1783593	99.16	29.00	0.50%	0.26%	0.98	0.000				132bp	188bp	67.9%	0.0%	97.1%	73.6%	88.8%	86.8M	46.1%	97.4%	23573.2Mb	195.6M	58.7%	97.8%	65.7%
aih-tih-sc-c55577-R1_B23WHYVLT4_1_1																																	132.5bp	97.5%	95.4M																89.0%	57%	151bp	151bp	27%	198.6M
aih-tih-sc-c55577-R1_B23WHYVLT4_1_2																																																			86.8%	58%	151bp	151bp	18%	198.6M
aih-tih-sc-c55577-R1_B23WHYVLT4_1_trimmed_1																																																			83.6%	58%	124bp	127bp	18%	97.8M	83.6%	58%	124bp	127bp	18%	97.8M
aih-tih-sc-c55577-R1_B23WHYVLT4_1_trimmed_2																																																			82.8%	59%	124bp	127bp	9%	97.8M	82.8%	59%	124bp	127bp	9%	97.8M
aih-tih-sc-c5cb90-R1_B23WHYVLT4_1	9999000000	92300320	121.1bp	114.0bp	0.09%	2.41%	0.80%	0.00%	0.16%	6.52%	1.18%	0.00%	No	0.00%	195743752	0.01%	0.55%	88013804	0.92%	0.95%	0.06%	0.05%	80976002	40638157	5402627	2166438	130.13	46.00	0.56%	0.34%	0.98	0.000				123bp	194bp	55.5%	0.0%	97.3%	80.2%	91.7%	89.7M	32.7%	97.5%	22581.0Mb	195.7M	56.1%	97.9%	74.7%
aih-tih-sc-c5cb90-R1_B23WHYVLT4_1_1																																	121.1bp	97.7%	95.6M																90.8%	54%	151bp	151bp	27%	513.3M
aih-tih-sc-c5cb90-R1_B23WHYVLT4_1_2																																																			89.0%	56%	151bp	151bp	18%	513.3M
aih-tih-sc-c5cb90-R1_B23WHYVLT4_1_trimmed_1																																																			77.5%	55%	118bp	117bp	18%	97.9M	77.5%	55%	118bp	117bp	18%	97.9M
aih-tih-sc-c5cb90-R1_B23WHYVLT4_1_trimmed_2																																																			76.9%	56%	118bp	122bp	9%	97.9M	76.9%	56%	118bp	122bp	9%	97.9M
aih-tih-sc-c63171-R1_B23WHYVLT4_1	9999000000	201278891	129.1bp	120.0bp	0.04%	1.13%	0.58%	0.00%	0.10%	3.41%	0.87%	0.00%	No	0.00%	196740290	0.01%	0.38%	121923545	0.94%	0.96%	0.05%	0.04%	114290606	57469416	6170016	2340565	200.27	90.00	0.65%	0.48%	0.98	0.000				135bp	249bp	38.3%	0.0%	97.0%	85.0%	93.8%	92.2M	22.0%	97.6%	23497.2Mb	196.7M	54.3%	98.4%	68.2%
aih-tih-sc-c63171-R1_B23WHYVLT4_1_1																																	129.1bp	97.7%	96.1M																84.4%	53%	151bp	151bp	18%	335.7M
aih-tih-sc-c63171-R1_B23WHYVLT4_1_2																																																			82.9%	54%	151bp	151bp	18%	335.7M
aih-tih-sc-c63171-R1_B23WHYVLT4_1_trimmed_1																																																			73.2%	53%	122bp	127bp	9%	98.4M	73.2%	53%	122bp	127bp	9%	98.4M
aih-tih-sc-c63171-R1_B23WHYVLT4_1_trimmed_2																																																			72.2%	54%	122bp	127bp	9%	98.4M	72.2%	54%	122bp	127bp	9%	98.4M
aih-tih-sc-c7b68f-R1_B23WHYVLT4_1	9999000000	211678053	130.4bp	121.0bp	0.06%	1.17%	0.88%	0.00%	0.13%	3.40%	1.22%	0.00%	No	0.00%	196715210	0.01%	0.38%	121074352	0.95%	0.97%	0.04%	0.03%	114593578	57643531	5401405	1931858	203.09	81.00	0.64%	0.46%	0.98	0.000				145bp	343bp	38.7%	0.0%	97.6%	91.0%	96.0%	94.4M	23.9%	97.7%	23543.2Mb	196.7M	52.9%	98.4%	66.8%
aih-tih-sc-c7b68f-R1_B23WHYVLT4_1_1																																	130.4bp	98.3%	96.7M																86.1%	52%	151bp	151bp	27%	322.9M
aih-tih-sc-c7b68f-R1_B23WHYVLT4_1_2																																																			84.5%	53%	151bp	151bp	18%	322.9M
aih-tih-sc-c7b68f-R1_B23WHYVLT4_1_trimmed_1																																																			76.3%	52%	122bp	127bp	18%	98.4M	76.3%	52%	122bp	127bp	18%	98.4M
aih-tih-sc-c7b68f-R1_B23WHYVLT4_1_trimmed_2																																																			75.6%	53%	123bp	127bp	9%	98.4M	75.6%	53%	123bp	127bp	9%	98.4M
aih-tih-sc-c9a01b-R1_B23WHYVLT4_1	9999000000	171846292	140.7bp	130.0bp	0.04%	0.76%	0.55%	0.00%	0.08%	2.52%	0.86%	0.00%	No	0.00%	196841658	0.01%	0.45%	107314630	0.94%	0.96%	0.05%	0.04%	100529260	50720330	5495346	1894445	191.27	79.00	0.65%	0.45%	0.98	0.000				189bp	439bp	45.8%	0.0%	96.9%	91.3%	96.3%	94.7M	30.7%	97.5%	24628.6Mb	196.8M	55.5%	98.4%	57.4%
aih-tih-sc-c9a01b-R1_B23WHYVLT4_1_1																																	140.7bp	98.0%	96.5M																90.0%	54%	151bp	151bp	18%	471.3M
aih-tih-sc-c9a01b-R1_B23WHYVLT4_1_2																																																			88.1%	55%	151bp	151bp	18%	471.3M
aih-tih-sc-c9a01b-R1_B23WHYVLT4_1_trimmed_1																																																			78.8%	55%	128bp	137bp	9%	98.4M	78.8%	55%	128bp	137bp	9%	98.4M
aih-tih-sc-c9a01b-R1_B23WHYVLT4_1_trimmed_2																																																			77.2%	55%	128bp	142bp	9%	98.4M	77.2%	55%	128bp	142bp	9%	98.4M
aih-tih-sc-ce6733-R1_B23WHYVLT4_1	9999000000	225452652	143.8bp	133.0bp	0.04%	1.08%	0.65%	0.00%	0.08%	3.37%	1.00%	0.00%	No	0.00%	196695882	0.01%	0.37%	124704856	0.94%	0.97%	0.05%	0.03%	117702273	59318686	5664371	1908147	226.54	89.00	0.65%	0.48%	0.98	0.000				168bp	355bp	36.9%	0.0%	97.2%	86.4%	94.3%	92.7M	24.5%	97.5%	24772.7Mb	196.7M	56.5%	98.3%	56.3%
aih-tih-sc-ce6733-R1_B23WHYVLT4_1_1																																	143.8bp	98.0%	96.4M																88.6%	55%	151bp	151bp	18%	523.9M
aih-tih-sc-ce6733-R1_B23WHYVLT4_1_2																																																			87.3%	56%	151bp	151bp	18%	523.9M
aih-tih-sc-ce6733-R1_B23WHYVLT4_1_trimmed_1																																																			75.7%	56%	129bp	142bp	9%	98.3M	75.7%	56%	129bp	142bp	9%	98.3M
aih-tih-sc-ce6733-R1_B23WHYVLT4_1_trimmed_2																																																			75.2%	56%	129bp	142bp	9%	98.3M	75.2%	56%	129bp	142bp	9%	98.3M
aih-tih-sc-d113b5-R1_B23WHYVLT4_1	9999000000	111688590	123.7bp	115.0bp	0.01%	0.97%	0.53%	0.00%	0.03%	3.09%	0.86%	0.00%	No	0.00%	196628356	0.01%	0.52%	95295715	0.93%	0.96%	0.05%	0.04%	89014937	44723720	4862830	1860693	149.57	69.00	0.64%	0.42%	0.98	0.000				143bp	288bp	51.9%	0.0%	97.3%	89.8%	95.5%	93.9M	30.9%	97.6%	22963.2Mb	196.6M	56.2%	98.3%	71.1%
aih-tih-sc-d113b5-R1_B23WHYVLT4_1_1																																	123.7bp	98.0%	96.3M																87.3%	54%	151bp	151bp	18%	313.6M
aih-tih-sc-d113b5-R1_B23WHYVLT4_1_2																																																			85.5%	55%	151bp	151bp	18%	313.6M
aih-tih-sc-d113b5-R1_B23WHYVLT4_1_trimmed_1																																																			76.9%	55%	120bp	122bp	18%	98.3M	76.9%	55%	120bp	122bp	18%	98.3M
aih-tih-sc-d113b5-R1_B23WHYVLT4_1_trimmed_2																																																			76.1%	56%	120bp	122bp	18%	98.3M	76.1%	56%	120bp	122bp	18%	98.3M
aih-tih-sc-d12f1f-R1_B23WHYVLT4_1	9999000000	314795022	145.5bp	131.0bp	0.02%	0.94%	0.59%	0.00%	0.04%	2.83%	0.79%	0.00%	No	0.00%	196778450	0.01%	0.31%	135436117	0.95%	0.97%	0.05%	0.03%	128351337	64740270	6098454	2014512	250.67	100.00	0.65%	0.50%	0.98	0.000				166bp	415bp	31.3%	0.0%	97.1%	91.0%	95.9%	94.4M	21.4%	97.8%	24812.2Mb	196.8M	52.3%	98.4%	54.7%
aih-tih-sc-d12f1f-R1_B23WHYVLT4_1_1																																	145.5bp	98.0%	96.5M																87.5%	51%	151bp	151bp	27%	493.1M
aih-tih-sc-d12f1f-R1_B23WHYVLT4_1_2																																																			85.9%	52%	151bp	151bp	18%	493.1M
aih-tih-sc-d12f1f-R1_B23WHYVLT4_1_trimmed_1																																																			75.8%	51%	129bp	142bp	18%	98.4M	75.8%	51%	129bp	142bp	18%	98.4M
aih-tih-sc-d12f1f-R1_B23WHYVLT4_1_trimmed_2																																																			74.6%	52%	129bp	142bp	9%	98.4M	74.6%	52%	129bp	142bp	9%	98.4M
aih-tih-sc-d2f1f4-R1_B23WHYVLT4_1	9999000000	121448779	135.0bp	126.0bp	0.02%	0.63%	0.36%	0.00%	0.05%	2.02%	0.57%	0.00%	No	0.00%	196648590	0.01%	0.49%	100831083	0.92%	0.95%	0.07%	0.05%	92535478	46561725	6608453	2636606	161.98	76.00	0.66%	0.44%	0.98	0.000				138bp	215bp	49.1%	0.0%	95.9%	88.5%	95.2%	93.6M	32.1%	97.7%	24005.5Mb	196.6M	55.4%	98.3%	64.6%
aih-tih-sc-d2f1f4-R1_B23WHYVLT4_1_1																																	135.0bp	97.2%	95.6M																82.1%	54%	151bp	151bp	27%	223.7M
aih-tih-sc-d2f1f4-R1_B23WHYVLT4_1_2																																																			79.8%	55%	151bp	151bp	18%	223.7M
aih-tih-sc-d2f1f4-R1_B23WHYVLT4_1_trimmed_1																																																			73.2%	55%	125bp	132bp	18%	98.3M	73.2%	55%	125bp	132bp	18%	98.3M
aih-tih-sc-d2f1f4-R1_B23WHYVLT4_1_trimmed_2																																																			71.6%	55%	125bp	132bp	9%	98.3M	71.6%	55%	125bp	132bp	9%	98.3M
aih-tih-sc-d5506e-R1_B23WHYVLT4_1	9999000000	89013755	130.7bp	121.0bp	0.03%	0.78%	0.32%	0.00%	0.06%	2.72%	0.54%	0.00%	No	0.00%	196469408	0.01%	0.58%	82029168	0.92%	0.95%	0.06%	0.05%	75597471	38011153	5028165	1952890	133.61	57.00	0.61%	0.37%	0.97	0.000				155bp	306bp	58.7%	0.0%	97.0%	90.0%	95.7%	94.0M	38.3%	97.4%	23713.3Mb	196.5M	56.0%	98.2%	65.1%
aih-tih-sc-d5506e-R1_B23WHYVLT4_1_1																																	130.7bp	97.9%	96.1M																88.6%	54%	151bp	151bp	36%	260.9M
aih-tih-sc-d5506e-R1_B23WHYVLT4_1_2																																																			86.3%	55%	151bp	151bp	18%	260.9M
aih-tih-sc-d5506e-R1_B23WHYVLT4_1_trimmed_1																																																			80.7%	55%	124bp	127bp	27%	98.2M	80.7%	55%	124bp	127bp	27%	98.2M
aih-tih-sc-d5506e-R1_B23WHYVLT4_1_trimmed_2																																																			79.2%	56%	124bp	132bp	18%	98.2M	79.2%	56%	124bp	132bp	18%	98.2M
aih-tih-sc-e9e54b-R1_B23WHYVLT4_1	9999000000	114829289	128.6bp	121.0bp	0.03%	1.17%	0.51%	0.00%	0.06%	3.47%	0.80%	0.00%	No	0.00%	196274056	0.01%	0.50%	98961586	0.92%	0.95%	0.06%	0.05%	91065863	45776201	6224200	2485681	153.15	64.00	0.63%	0.41%	0.98	0.000				131bp	204bp	50.0%	0.0%	96.4%	86.6%	94.5%	92.8M	32.0%	97.5%	23397.9Mb	196.3M	56.6%	98.1%	69.1%
aih-tih-sc-e9e54b-R1_B23WHYVLT4_1_1																																	128.6bp	97.2%	95.4M																85.4%	55%	151bp	151bp	18%	295.6M
aih-tih-sc-e9e54b-R1_B23WHYVLT4_1_2																																																			83.4%	56%	151bp	151bp	18%	295.6M
aih-tih-sc-e9e54b-R1_B23WHYVLT4_1_trimmed_1																																																			74.1%	56%	122bp	127bp	9%	98.1M	74.1%	56%	122bp	127bp	9%	98.1M
aih-tih-sc-e9e54b-R1_B23WHYVLT4_1_trimmed_2																																																			73.4%	56%	122bp	127bp	9%	98.1M	73.4%	56%	122bp	127bp	9%	98.1M
aih-tih-sc-ea2fad-R1_B23WHYVLT4_1	9999000000	80086951	111.3bp	105.0bp	0.04%	1.16%	0.58%	0.00%	0.09%	3.51%	0.88%	0.00%	No	0.00%	196783842	0.01%	0.61%	77204392	0.92%	0.94%	0.07%	0.06%	70802889	35506421	5352550	2272420	105.48	41.00	0.56%	0.30%	0.98	0.000				115bp	193bp	61.1%	0.0%	97.2%	90.1%	96.0%	94.4M	35.9%	97.8%	21505.0Mb	196.8M	52.5%	98.4%	82.0%
aih-tih-sc-ea2fad-R1_B23WHYVLT4_1_1																																	111.3bp	97.9%	96.3M																84.8%	51%	151bp	151bp	18%	186.9M
aih-tih-sc-ea2fad-R1_B23WHYVLT4_1_2																																																			82.9%	52%	151bp	151bp	27%	186.9M
aih-tih-sc-ea2fad-R1_B23WHYVLT4_1_trimmed_1																																																			78.9%	52%	112bp	112bp	9%	98.4M	78.9%	52%	112bp	112bp	9%	98.4M
aih-tih-sc-ea2fad-R1_B23WHYVLT4_1_trimmed_2																																																			77.7%	52%	112bp	112bp	9%	98.4M	77.7%	52%	112bp	112bp	9%	98.4M
aih-tih-sc-ea6ef3-R1_B23WHYVLT4_1	9999000000	245323975	136.7bp	126.0bp	0.04%	1.19%	0.67%	0.00%	0.09%	3.55%	0.97%	0.00%	No	0.00%	196539280	0.01%	0.35%	127196429	0.94%	0.97%	0.05%	0.03%	119882556	60350432	5975093	2107560	220.28	91.00	0.65%	0.48%	0.98	0.000				147bp	309bp	35.5%	0.0%	97.1%	85.0%	93.6%	92.0M	22.3%	97.7%	24074.2Mb	196.5M	53.2%	98.3%	62.8%
aih-tih-sc-ea6ef3-R1_B23WHYVLT4_1_1																																	136.7bp	97.9%	96.2M																86.9%	52%	151bp	151bp	27%	448.5M
aih-tih-sc-ea6ef3-R1_B23WHYVLT4_1_2																																																			85.2%	53%	151bp	151bp	18%	448.5M
aih-tih-sc-ea6ef3-R1_B23WHYVLT4_1_trimmed_1																																																			74.9%	52%	125bp	132bp	18%	98.3M	74.9%	52%	125bp	132bp	18%	98.3M
aih-tih-sc-ea6ef3-R1_B23WHYVLT4_1_trimmed_2																																																			73.9%	53%	125bp	132bp	9%	98.3M	73.9%	53%	125bp	132bp	9%	98.3M
aih-tih-sc-f14fdc-R1_B23WHYVLT4_1	9999000000	295858621	141.5bp	130.0bp	0.02%	0.82%	0.43%	0.00%	0.04%	2.77%	0.70%	0.00%	No	0.00%	196902336	0.01%	0.35%	128708358	0.94%	0.96%	0.05%	0.04%	121050848	61024978	6321480	2215269	232.80	105.00	0.69%	0.51%	0.98	0.000				174bp	402bp	34.9%	0.0%	96.8%	90.9%	96.0%	94.5M	22.7%	97.6%	24644.5Mb	196.9M	53.5%	98.5%	57.1%
aih-tih-sc-f14fdc-R1_B23WHYVLT4_1_1																																	141.5bp	97.9%	96.4M																86.7%	52%	151bp	151bp	18%	438.8M
aih-tih-sc-f14fdc-R1_B23WHYVLT4_1_2																																																			85.5%	53%	151bp	151bp	18%	438.8M
aih-tih-sc-f14fdc-R1_B23WHYVLT4_1_trimmed_1																																																			75.4%	53%	128bp	137bp	9%	98.5M	75.4%	53%	128bp	137bp	9%	98.5M
aih-tih-sc-f14fdc-R1_B23WHYVLT4_1_trimmed_2																																																			74.6%	53%	128bp	142bp	9%	98.5M	74.6%	53%	128bp	142bp	9%	98.5M
aih-tih-sc-f39d87-R1_B23WHYVLT4_1	9999000000	163399095	119.3bp	111.0bp	0.08%	1.85%	0.56%	0.00%	0.16%	5.32%	0.86%	0.00%	No	0.00%	196767324	0.01%	0.42%	114362168	0.93%	0.96%	0.05%	0.04%	106574373	53532042	6280071	2498028	172.49	71.00	0.62%	0.44%	0.98	0.000				118bp	187bp	42.2%	0.0%	96.9%	86.2%	94.3%	92.8M	23.1%	97.7%	22261.6Mb	196.8M	52.9%	98.4%	75.7%
aih-tih-sc-f39d87-R1_B23WHYVLT4_1_1																																	119.3bp	97.9%	96.3M																82.8%	51%	151bp	151bp	27%	233.8M
aih-tih-sc-f39d87-R1_B23WHYVLT4_1_2																																																			80.8%	53%	151bp	151bp	18%	233.8M
aih-tih-sc-f39d87-R1_B23WHYVLT4_1_trimmed_1																																																			74.7%	52%	116bp	117bp	18%	98.4M	74.7%	52%	116bp	117bp	18%	98.4M
aih-tih-sc-f39d87-R1_B23WHYVLT4_1_trimmed_2																																																			73.1%	53%	116bp	117bp	9%	98.4M	73.1%	53%	116bp	117bp	9%	98.4M
aih-tih-sc-f4e5c4-R1_B23WHYVLT4_1	9999000000	72005912	119.7bp	113.0bp	0.02%	0.68%	0.30%	0.00%	0.04%	2.20%	0.47%	0.00%	No	0.00%	195835044	0.01%	0.65%	68537597	0.87%	0.90%	0.10%	0.10%	59533993	29854955	7177836	3204376	91.22	40.00	0.56%	0.28%	0.98	0.000				117bp	160bp	65.6%	0.0%	95.7%	85.8%	94.3%	92.3M	39.4%	97.7%	22225.7Mb	195.8M	54.0%	97.9%	77.1%
aih-tih-sc-f4e5c4-R1_B23WHYVLT4_1_1																																	119.7bp	96.4%	94.4M																85.0%	53%	151bp	151bp	18%	170.9M
aih-tih-sc-f4e5c4-R1_B23WHYVLT4_1_2																																																			83.4%	54%	151bp	151bp	36%	170.9M
aih-tih-sc-f4e5c4-R1_B23WHYVLT4_1_trimmed_1																																																			79.6%	53%	116bp	117bp	9%	97.9M	79.6%	53%	116bp	117bp	9%	97.9M
aih-tih-sc-f4e5c4-R1_B23WHYVLT4_1_trimmed_2																																																			79.0%	54%	116bp	117bp	9%	97.9M	79.0%	54%	116bp	117bp	9%	97.9M
aih-tih-sc-f71b32-R1_B23WHYVLT4_1	9999000000	279934603	147.7bp	135.0bp	0.03%	1.38%	0.51%	0.00%	0.06%	4.07%	0.74%	0.00%	No	0.00%	196801218	0.01%	0.32%	133540290	0.94%	0.96%	0.05%	0.04%	125550573	63373827	6843142	2340582	244.09	107.00	0.66%	0.51%	0.98	0.000				171bp	392bp	32.3%	0.0%	96.3%	88.4%	95.1%	93.6M	22.1%	97.7%	24985.9Mb	196.8M	53.6%	98.4%	54.1%
aih-tih-sc-f71b32-R1_B23WHYVLT4_1_1																																	147.7bp	97.6%	96.0M																85.0%	52%	151bp	151bp	18%	317.6M
aih-tih-sc-f71b32-R1_B23WHYVLT4_1_2																																																			83.0%	53%	151bp	151bp	18%	317.6M
aih-tih-sc-f71b32-R1_B23WHYVLT4_1_trimmed_1																																																			75.1%	53%	130bp	142bp	9%	98.4M	75.1%	53%	130bp	142bp	9%	98.4M
aih-tih-sc-f71b32-R1_B23WHYVLT4_1_trimmed_2																																																			73.7%	53%	130bp	142bp	9%	98.4M	73.7%	53%	130bp	142bp	9%	98.4M

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order. Table ID: table-general_stats_table

Sort	Group	Column	Description	ID	Scale
\|\|	Custom content: Preseq	Total Reads	Total reads from preseq analysis	`total_reads`	None
\|\|	Custom content: Preseq	Expected Distinct	Expected distinct molecules at maximum sequencing depth	`expected_distinct`	None
\|\|	Custom content: Kallisto	Fragment Length Mean	Mean fragment length from kallisto fragment length distribution	`fragment_length_mean`	None
\|\|	Custom content: Kallisto	Fragment Length Median	Median fragment length from kallisto fragment length distribution	`fragment_length_median`	None
\|\|	Custom content: Expression QC	pct Mito est. counts	Percentage of estimated counts from mitochondrial genes; high values can indicate cell damage or stress.	`pct_mito_est_counts`	None
\|\|	Custom content: Expression QC	pct Ribo est. counts	Percentage of estimated counts from ribosomal genes; useful for assessing RNA quality and technical variation.	`pct_ribo_est_counts`	None
\|\|	Custom content: Expression QC	pct Housekeeping est. counts	Percentage of estimated counts from housekeeping genes.	`pct_hk_est_counts`	None
\|\|	Custom content: Expression QC	pct Male est. counts	pct Male est. counts	`pct_male_est_counts`	None
\|\|	Custom content: Expression QC	pct Mito TPM	Percentage of TPM from mitochondrial genes; high values can indicate cell damage or stress.	`pct_mito_tpm`	None
\|\|	Custom content: Expression QC	pct Ribo TPM	Percentage of TPM from ribosomal genes; useful for assessing RNA quality and technical variation.	`pct_ribo_tpm`	None
\|\|	Custom content: Expression QC	pct Housekeeping TPM	Percentage of TPM from housekeeping genes.	`pct_hk_tpm`	None
\|\|	Custom content: Expression QC	pct Male TPM	pct Male TPM	`pct_male_tpm`	None
\|\|	Custom content: RNA contamination	RNA contaminated	Whether the sample was classified as contaminated from VAF_p90	`is_contaminated`	None
\|\|	Custom content: RNA contamination	RNA contamination %	Estimated RNA contamination from VAF_p90; 0% for samples classified as clean	`contamination_pct`	None
\|\|	Custom content: SeqTools (general stats)	Reads	SeqTools (general stats) — reads	`reads`	None
\|\|	Custom content: SeqTools (general stats)	Frac Unmapped	SeqTools (general stats) — frac unmapped	`frac_unmapped`	None
\|\|	Custom content: SeqTools (general stats)	Frac Dupes	SeqTools (general stats) — frac dupes	`frac_dupes`	None
\|\|	Custom content: SeqTools (general stats)	Unique Reads	SeqTools (general stats) — unique reads	`unique_reads`	None
\|\|	Custom content: SeqTools (general stats)	Frac Reads On Target	SeqTools (general stats) — frac reads on target	`frac_reads_on_target`	None
\|\|	Custom content: SeqTools (general stats)	Frac Pairs On Target	SeqTools (general stats) — frac pairs on target	`frac_pairs_on_target`	None
\|\|	Custom content: SeqTools (general stats)	Frac Reads Off Target	SeqTools (general stats) — frac reads off target	`frac_reads_off_target`	None
\|\|	Custom content: SeqTools (general stats)	Frac Pairs Off Target	SeqTools (general stats) — frac pairs off target	`frac_pairs_off_target`	None
\|\|	Custom content: SeqTools (general stats)	Reads On Target	SeqTools (general stats) — reads on target	`reads_on_target`	None
\|\|	Custom content: SeqTools (general stats)	Pairs On Target	SeqTools (general stats) — pairs on target	`pairs_on_target`	None
\|\|	Custom content: SeqTools (general stats)	Reads Off Target	SeqTools (general stats) — reads off target	`reads_off_target`	None
\|\|	Custom content: SeqTools (general stats)	Pairs Off Target	SeqTools (general stats) — pairs off target	`pairs_off_target`	None
\|\|	Custom content: SeqTools (general stats)	Mean Depth	SeqTools (general stats) — mean depth	`mean_depth`	None
\|\|	Custom content: SeqTools (general stats)	Q50 Depth	SeqTools (general stats) — q50 depth	`q50_depth`	None
\|\|	Custom content: SeqTools (general stats)	Frac Bases Gt30X	SeqTools (general stats) — frac bases gt30x	`frac_bases_gt30x`	None
\|\|	Custom content: SeqTools (general stats)	Frac Bases Gt100X	SeqTools (general stats) — frac bases gt100x	`frac_bases_gt100x`	None
\|\|	Custom content: SeqTools (general stats)	N Q30	SeqTools (general stats) — n q30	`n_q30`	None
\|\|	Custom content: SeqTools (general stats)	GC Bias	SeqTools (general stats) — Ratio of high GC (63-100%) to low GC (0-43%) reads	`gc_bias`	None
\|\|	Kallisto	Frag Length	Estimated average fragment length	`fragment_length`	None
\|\|	Kallisto	% Aligned	% processed reads that were pseudoaligned	`percent_aligned`	None
\|\|	Kallisto	M Aligned	Pseudoaligned reads (millions)	`pseudoaligned_reads`	read_count
\|\|	Picard: InsertSizeMetrics	Insert Size	Median Insert Size, all read orientations (bp)	`summed_median`	None
\|\|	Picard: InsertSizeMetrics	Mean Insert Size	Mean Insert Size, all read orientations (bp)	`summed_mean`	None
\|\|	Picard: Mark Duplicates	Duplication	Mark Duplicates - Percent Duplication	`PERCENT_DUPLICATION`	None
\|\|	Picard: RnaSeqMetrics	rRNA	Percent of aligned bases overlapping ribosomal RNA regions	`PCT_RIBOSOMAL_BASES`	None
\|\|	Picard: RnaSeqMetrics	mRNA	Percent of aligned bases overlapping UTRs and coding regions of mRNA transcripts	`PCT_MRNA_BASES`	None
\|\|	RSeQC	% Proper Pairs	% Reads mapped in proper pairs	`proper_pairs_percent`	None
\|\|	STAR	% Aligned	% Uniquely mapped reads	`uniquely_mapped_percent`	None
\|\|	STAR	M Aligned	Uniquely mapped reads (millions)	`uniquely_mapped`	read_count
\|\|	fastp	% Duplication	Duplication rate before filtering	`pct_duplication`	None
\|\|	fastp	% > Q30	Percentage of reads > Q30 after filtering	`after_filtering_q30_rate`	None
\|\|	fastp	Mb Q30 bases	Bases > Q30 after filtering (millions)	`after_filtering_q30_bases`	base_count
\|\|	fastp	M Reads After Filtering	Total reads after filtering (millions)	`filtering_result_passed_filter_reads`	read_count
\|\|	fastp	GC content	GC content after filtering	`after_filtering_gc_content`	None
\|\|	fastp	% PF	Percent reads passing filter	`pct_surviving`	None
\|\|	fastp	% Adapter	Percentage adapter-trimmed reads	`pct_adapter`	None
\|\|	FastQC (raw)	% Dups	% Duplicate Reads	`percent_duplicates`	None
\|\|	FastQC (raw)	% GC	Average % GC Content	`percent_gc`	None
\|\|	FastQC (raw)	Average Read Length	Average Read Length (bp)	`avg_sequence_length`	None
\|\|	FastQC (raw)	Median Read Length	Median Read Length (bp)	`median_sequence_length`	None
\|\|	FastQC (raw)	% Failed	Percentage of modules failed in FastQC report (includes those not plotted here)	`percent_fails`	None
\|\|	FastQC (raw)	M Seqs	Total Sequences (millions)	`total_sequences`	read_count
\|\|	FastQC (trimmed)	% Dups	% Duplicate Reads	`percent_duplicates`	None
\|\|	FastQC (trimmed)	% GC	Average % GC Content	`percent_gc`	None
\|\|	FastQC (trimmed)	Average Read Length	Average Read Length (bp)	`avg_sequence_length`	None
\|\|	FastQC (trimmed)	Median Read Length	Median Read Length (bp)	`median_sequence_length`	None
\|\|	FastQC (trimmed)	% Failed	Percentage of modules failed in FastQC report (includes those not plotted here)	`percent_fails`	None
\|\|	FastQC (trimmed)	M Seqs	Total Sequences (millions)	`total_sequences`	read_count

Kallisto

Kallisto is a program for quantifying abundances of transcripts from RNA-Seq data.DOI: 10.1038/nbt.3519.

Created with MultiQC

Picard

Picard is a set of Java command line tools for manipulating high-throughput sequencing data.

Insert Size

Plot shows the number of reads at a given insert size. Reads with different orientations are summed.

Created with MultiQC

Mark Duplicates

Number of reads, categorised by duplication state. Pair counts are doubled - see help text for details.

The table in the Picard metrics file contains some columns referring read pairs and some referring to single reads.

To make the numbers in this plot sum correctly, values referring to pairs are doubled according to the scheme below:

READS_IN_DUPLICATE_PAIRS = 2 * READ_PAIR_DUPLICATES
READS_IN_UNIQUE_PAIRS = 2 * (READ_PAIRS_EXAMINED - READ_PAIR_DUPLICATES)
READS_IN_UNIQUE_UNPAIRED = UNPAIRED_READS_EXAMINED - UNPAIRED_READ_DUPLICATES
READS_IN_DUPLICATE_PAIRS_OPTICAL = 2 * READ_PAIR_OPTICAL_DUPLICATES
READS_IN_DUPLICATE_PAIRS_NONOPTICAL = READS_IN_DUPLICATE_PAIRS - READS_IN_DUPLICATE_PAIRS_OPTICAL
READS_IN_DUPLICATE_UNPAIRED = UNPAIRED_READ_DUPLICATES
READS_UNMAPPED = UNMAPPED_READS

Created with MultiQC

RnaSeqMetrics Assignment

Number of bases in primary alignments that align to regions in the reference genome.

Created with MultiQC

Gene Coverage

Created with MultiQC

Preseq

Preseq estimates the complexity of a library, showing how many additional unique reads are sequenced for increasing total read count. A shallow curve indicates complexity saturation. The dashed line shows a perfectly complex library where total reads = unique reads.DOI: 10.1038/nmeth.2375.

Complexity curve (molecule count)

Note that the x-axis is trimmed at the point where all the datasets show 80% of their maximum y-value, to avoid ridiculous scales.

Created with MultiQC

RSeQC

RSeQC package provides a number of useful modules that can comprehensively evaluate high throughput RNA-seq data.DOI: 10.1093/bioinformatics/bts356.

Read Distribution

Read Distribution calculates how mapped reads are distributed over genome features.

Created with MultiQC

Inner Distance

Inner Distance calculates the inner distance (or insert size) between two paired RNA reads. Note that this can be negative if fragments overlap.

Created with MultiQC

Read Duplication

read_duplication.py calculates how many alignment positions have a certain number of exact duplicates. Note - plot truncated at 500 occurrences and binned.

Created with MultiQC

Junction Annotation

Junction annotation compares detected splice junctions to a reference gene model. An RNA read can be spliced 2 or more times, each time is called a splicing event.

Created with MultiQC

Junction Saturation

Junction Saturation counts the number of known splicing junctions that are observed in each dataset. If sequencing depth is sufficient, all (annotated) splice junctions should be rediscovered, resulting in a curve that reaches a plateau. Missing low abundance splice junctions can affect downstream analysis.

Click a line to see the data side by side (as in the original RSeQC plot).

Created with MultiQC

Infer experiment

Infer experiment counts the percentage of reads and read pairs that match the strandedness of overlapping transcripts. It can be used to infer whether RNA-seq library preps are stranded (sense or antisense).

Created with MultiQC

Bam Stat

All numbers reported in millions.

Created with MultiQC

Showing ⁹⁶/₉₆ rows and ¹⁴/₁₄ columns.

Sample Name	Total records	QC failed	Duplicates	Non primary hit	Unmapped	Unique	Read-1	Read-2	+ve strand	-ve strand	Non-splice reads	Splice reads	Proper pairs	Different chrom
FFPE_HD789_01_RNA_13_B23WHYVLT4_1	208.6M	0.0M	0.0M	11.2M	1.1M	190.7M	95.4M	95.3M	95.4M	95.3M	85.4M	105.3M	190.1M	0.0M
FFPE_V4_0002_RNA_0001_B23WHYVLT4_1	208.8M	0.0M	0.0M	11.4M	1.2M	190.1M	95.1M	95.0M	95.1M	95.0M	88.6M	101.6M	189.5M	0.0M
NTC_0001_0001_B23WHYVLT4_1	0.1M	0.0M	0.0M	0.1M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M
aih-tih-sc-0183f6-R1_B23WHYVLT4_1	222.4M	0.0M	0.0M	25.2M	1.6M	184.2M	92.2M	92.1M	92.1M	92.1M	112.1M	72.2M	183.6M	0.0M
aih-tih-sc-027d27-R1_B23WHYVLT4_1	213.8M	0.0M	0.0M	16.5M	1.4M	188.3M	94.2M	94.1M	94.2M	94.1M	106.6M	81.7M	187.7M	0.0M
aih-tih-sc-03bdd8-R1_B23WHYVLT4_1	214.9M	0.0M	0.0M	17.6M	1.4M	187.8M	94.0M	93.8M	93.9M	93.9M	100.0M	87.8M	187.2M	0.0M
aih-tih-sc-070e1d-R1_B23WHYVLT4_1	214.5M	0.0M	0.0M	17.5M	1.3M	187.7M	93.9M	93.8M	93.9M	93.8M	103.1M	84.6M	187.0M	0.0M
aih-tih-sc-099ac4-R1_B23WHYVLT4_1	212.5M	0.0M	0.0M	15.0M	1.6M	189.0M	94.5M	94.4M	94.5M	94.5M	100.6M	88.3M	188.4M	0.0M
aih-tih-sc-0a6b7b-R1_B23WHYVLT4_1	214.3M	0.0M	0.0M	16.8M	1.2M	188.1M	94.1M	94.0M	94.0M	94.0M	94.8M	93.2M	187.5M	0.0M
aih-tih-sc-0fc41c-R1_B23WHYVLT4_1	216.3M	0.0M	0.0M	18.9M	1.7M	186.9M	93.5M	93.4M	93.4M	93.4M	105.1M	81.8M	186.3M	0.0M
aih-tih-sc-13a2f6-R1_B23WHYVLT4_1	215.1M	0.0M	0.0M	17.8M	1.8M	187.7M	93.9M	93.7M	93.9M	93.8M	108.7M	79.0M	187.0M	0.0M
aih-tih-sc-16054b-R1_B23WHYVLT4_1	210.1M	0.0M	0.0M	12.4M	1.3M	190.3M	95.2M	95.1M	95.2M	95.1M	94.3M	96.1M	189.7M	0.0M
aih-tih-sc-176637-R1_B23WHYVLT4_1	210.4M	0.0M	0.0M	13.3M	1.3M	189.6M	94.8M	94.7M	94.8M	94.7M	95.5M	94.0M	188.9M	0.0M
aih-tih-sc-18a6f2-R1_B23WHYVLT4_1	210.5M	0.0M	0.0M	12.9M	1.3M	190.4M	95.3M	95.1M	95.2M	95.2M	105.2M	85.2M	189.7M	0.0M
aih-tih-sc-1ba302-R1_B23WHYVLT4_1	211.6M	0.0M	0.0M	13.8M	1.2M	190.2M	95.1M	95.0M	95.1M	95.1M	93.0M	97.1M	189.6M	0.0M
aih-tih-sc-1d89ab-R1_B23WHYVLT4_1	212.0M	0.0M	0.0M	14.5M	1.4M	189.2M	94.7M	94.5M	94.6M	94.6M	102.4M	86.8M	188.6M	0.0M
aih-tih-sc-1d9134-R1_B23WHYVLT4_1	209.1M	0.0M	0.0M	11.2M	1.1M	191.3M	95.7M	95.6M	95.7M	95.6M	92.2M	99.1M	190.6M	0.0M
aih-tih-sc-20b7e4-R1_B23WHYVLT4_1	215.9M	0.0M	0.0M	19.0M	1.7M	186.3M	93.2M	93.0M	93.1M	93.1M	117.7M	68.6M	185.6M	0.0M
aih-tih-sc-2a5468-R1_B23WHYVLT4_1	211.0M	0.0M	0.0M	14.6M	1.3M	188.0M	94.1M	94.0M	94.0M	94.0M	95.6M	92.4M	187.5M	0.0M
aih-tih-sc-2f84cc-R1_B23WHYVLT4_1	214.7M	0.0M	0.0M	17.6M	1.9M	187.3M	93.7M	93.6M	93.7M	93.6M	114.0M	73.3M	186.7M	0.0M
aih-tih-sc-3376ed-R1_B23WHYVLT4_1	209.0M	0.0M	0.0M	11.8M	1.2M	190.1M	95.1M	95.0M	95.1M	95.0M	90.4M	99.7M	189.4M	0.0M
aih-tih-sc-37dd87-R1_B23WHYVLT4_1	210.3M	0.0M	0.0M	12.6M	1.2M	190.2M	95.2M	95.1M	95.1M	95.1M	96.5M	93.8M	189.7M	0.0M
aih-tih-sc-3924cb-R1_B23WHYVLT4_1	208.7M	0.0M	0.0M	11.2M	1.2M	190.7M	95.4M	95.3M	95.4M	95.3M	96.1M	94.6M	190.1M	0.0M
aih-tih-sc-3b3c1e-R1_B23WHYVLT4_1	211.4M	0.0M	0.0M	13.9M	1.4M	189.6M	94.9M	94.8M	94.8M	94.8M	107.2M	82.4M	189.0M	0.0M
aih-tih-sc-3e91d2-R1_B23WHYVLT4_1	211.3M	0.0M	0.0M	13.7M	1.7M	188.6M	94.3M	94.3M	94.3M	94.3M	91.2M	97.5M	187.9M	0.0M
aih-tih-sc-445ab1-R1_B23WHYVLT4_1	210.6M	0.0M	0.0M	13.4M	1.3M	189.8M	95.0M	94.9M	94.9M	94.9M	95.7M	94.1M	189.3M	0.0M
aih-tih-sc-446670-R1_B23WHYVLT4_1	213.9M	0.0M	0.0M	16.6M	1.6M	186.9M	93.5M	93.4M	93.5M	93.4M	97.7M	89.2M	186.4M	0.0M
aih-tih-sc-4cd8a8-R1_B23WHYVLT4_1	215.6M	0.0M	0.0M	18.0M	1.4M	187.3M	93.7M	93.6M	93.7M	93.6M	109.9M	77.4M	186.6M	0.0M
aih-tih-sc-4dcd88-R1_B23WHYVLT4_1	210.5M	0.0M	0.0M	12.9M	1.3M	190.3M	95.2M	95.1M	95.2M	95.1M	100.5M	89.8M	189.6M	0.0M
aih-tih-sc-4f4d6e-R1_B23WHYVLT4_1	208.1M	0.0M	0.0M	9.9M	1.0M	192.0M	96.0M	96.0M	96.0M	96.0M	96.0M	96.1M	191.3M	0.0M
aih-tih-sc-505123-R1_B23WHYVLT4_1	214.8M	0.0M	0.0M	17.6M	1.4M	187.9M	94.0M	93.9M	93.9M	93.9M	123.7M	64.2M	187.2M	0.0M
aih-tih-sc-51d534-R1_B23WHYVLT4_1	214.7M	0.0M	0.0M	17.2M	1.1M	187.7M	93.9M	93.8M	93.9M	93.8M	94.9M	92.8M	187.1M	0.0M
aih-tih-sc-51ee59-R1_B23WHYVLT4_1	208.5M	0.0M	0.0M	10.9M	1.2M	190.9M	95.5M	95.4M	95.5M	95.5M	94.9M	96.0M	190.4M	0.0M
aih-tih-sc-522d83-R1_B23WHYVLT4_1	211.5M	0.0M	0.0M	14.4M	1.3M	188.7M	94.4M	94.3M	94.4M	94.3M	94.9M	93.8M	188.1M	0.0M
aih-tih-sc-5c902f-R1_B23WHYVLT4_1	222.8M	0.0M	0.0M	26.4M	2.1M	182.3M	91.2M	91.0M	91.1M	91.1M	111.5M	70.7M	181.6M	0.0M
aih-tih-sc-5ef3cb-R1_B23WHYVLT4_1	214.3M	0.0M	0.0M	17.7M	1.8M	187.6M	93.9M	93.7M	93.8M	93.8M	119.3M	68.3M	187.0M	0.0M
aih-tih-sc-60f9b9-R1_B23WHYVLT4_1	215.1M	0.0M	0.0M	17.9M	1.7M	187.1M	93.6M	93.5M	93.6M	93.5M	104.2M	82.9M	186.5M	0.0M
aih-tih-sc-6284ec-R1_B23WHYVLT4_1	211.2M	0.0M	0.0M	13.6M	1.4M	189.9M	95.0M	94.9M	95.0M	94.9M	99.8M	90.1M	189.3M	0.0M
aih-tih-sc-62dadb-R1_B23WHYVLT4_1	197.9M	0.0M	0.0M	17.5M	1.6M	171.2M	85.7M	85.5M	85.6M	85.6M	106.7M	64.5M	170.6M	0.0M
aih-tih-sc-63b0e7-R1_B23WHYVLT4_1	209.8M	0.0M	0.0M	12.4M	1.4M	190.0M	95.1M	95.0M	95.0M	95.0M	99.5M	90.6M	189.4M	0.0M
aih-tih-sc-63c8b4-R1_B23WHYVLT4_1	209.2M	0.0M	0.0M	11.9M	1.4M	190.4M	95.3M	95.1M	95.2M	95.2M	96.8M	93.6M	189.8M	0.0M
aih-tih-sc-6473fb-R1_B23WHYVLT4_1	213.1M	0.0M	0.0M	15.7M	1.4M	188.5M	94.3M	94.2M	94.3M	94.2M	110.7M	77.8M	187.8M	0.0M
aih-tih-sc-6e7384-R1_B23WHYVLT4_1	208.0M	0.0M	0.0M	10.4M	1.0M	191.2M	95.6M	95.6M	95.6M	95.6M	92.5M	98.7M	190.6M	0.0M
aih-tih-sc-72546a-R1_B23WHYVLT4_1	210.2M	0.0M	0.0M	12.7M	1.4M	190.3M	95.2M	95.1M	95.2M	95.1M	100.5M	89.8M	189.7M	0.0M
aih-tih-sc-758b5a-R1_B23WHYVLT4_1	211.9M	0.0M	0.0M	14.4M	1.3M	188.8M	94.4M	94.3M	94.4M	94.4M	94.4M	94.4M	188.1M	0.0M
aih-tih-sc-75c3a9-R1_B23WHYVLT4_1	210.9M	0.0M	0.0M	13.5M	1.2M	189.8M	95.0M	94.8M	94.9M	94.9M	98.0M	91.8M	189.1M	0.0M
aih-tih-sc-762402-R1_B23WHYVLT4_1	207.1M	0.0M	0.0M	9.5M	1.3M	191.6M	95.9M	95.8M	95.9M	95.8M	86.0M	105.7M	191.0M	0.0M
aih-tih-sc-76a805-R1_B23WHYVLT4_1	217.7M	0.0M	0.0M	20.6M	1.4M	186.0M	93.1M	93.0M	93.0M	93.0M	97.7M	88.3M	185.5M	0.0M
aih-tih-sc-79f487-R1_B23WHYVLT4_1	214.8M	0.0M	0.0M	17.4M	1.3M	186.8M	93.4M	93.4M	93.4M	93.4M	92.2M	94.7M	186.3M	0.0M
aih-tih-sc-7c610c-R1_B23WHYVLT4_1	221.1M	0.0M	0.0M	23.8M	1.5M	184.1M	92.1M	92.0M	92.1M	92.0M	102.2M	81.9M	183.5M	0.0M
aih-tih-sc-7c6c07-R1_B23WHYVLT4_1	212.7M	0.0M	0.0M	15.2M	1.2M	188.5M	94.3M	94.2M	94.3M	94.3M	98.6M	89.9M	187.9M	0.0M
aih-tih-sc-7c8bae-R1_B23WHYVLT4_1	208.8M	0.0M	0.0M	11.8M	1.0M	190.1M	95.1M	95.0M	95.1M	95.0M	90.2M	99.9M	189.5M	0.0M
aih-tih-sc-7d62c4-R1_B23WHYVLT4_1	211.3M	0.0M	0.0M	13.8M	1.4M	189.7M	94.9M	94.8M	94.9M	94.8M	95.6M	94.1M	189.0M	0.0M
aih-tih-sc-7dd1d5-R1_B23WHYVLT4_1	213.6M	0.0M	0.0M	16.6M	1.6M	187.8M	94.0M	93.8M	93.9M	93.9M	105.4M	82.4M	187.1M	0.0M
aih-tih-sc-7fc50e-R1_B23WHYVLT4_1	213.4M	0.0M	0.0M	15.9M	1.5M	188.7M	94.4M	94.3M	94.3M	94.3M	106.9M	81.8M	188.1M	0.0M
aih-tih-sc-82e8c1-R1_B23WHYVLT4_1	210.1M	0.0M	0.0M	12.2M	1.0M	190.6M	95.3M	95.2M	95.3M	95.3M	103.1M	87.5M	189.7M	0.0M
aih-tih-sc-884b4c-R1_B23WHYVLT4_1	213.6M	0.0M	0.0M	16.8M	1.7M	188.0M	94.1M	93.9M	94.1M	94.0M	96.7M	91.3M	187.4M	0.0M
aih-tih-sc-89cc59-R1_B23WHYVLT4_1	222.8M	0.0M	0.0M	26.8M	2.8M	182.6M	91.4M	91.2M	91.3M	91.2M	105.9M	76.7M	181.7M	0.0M
aih-tih-sc-8a623b-R1_B23WHYVLT4_1	210.2M	0.0M	0.0M	12.8M	1.3M	190.2M	95.1M	95.0M	95.1M	95.1M	100.4M	89.8M	189.6M	0.0M
aih-tih-sc-8b4971-R1_B23WHYVLT4_1	215.3M	0.0M	0.0M	17.7M	1.3M	188.6M	94.3M	94.2M	94.3M	94.3M	105.1M	83.5M	188.0M	0.0M
aih-tih-sc-8b9375-R1_B23WHYVLT4_1	210.3M	0.0M	0.0M	13.1M	1.6M	189.2M	94.7M	94.6M	94.6M	94.6M	98.9M	90.3M	188.5M	0.0M
aih-tih-sc-8d3f7c-R1_B23WHYVLT4_1	213.9M	0.0M	0.0M	16.3M	1.3M	188.8M	94.4M	94.3M	94.4M	94.4M	112.3M	76.5M	188.2M	0.0M
aih-tih-sc-910896-R1_B23WHYVLT4_1	213.3M	0.0M	0.0M	16.1M	1.3M	188.8M	94.5M	94.3M	94.4M	94.4M	105.2M	83.6M	188.2M	0.0M
aih-tih-sc-9114fc-R1_B23WHYVLT4_1	210.7M	0.0M	0.0M	13.4M	1.2M	190.1M	95.1M	95.0M	95.1M	95.0M	95.4M	94.7M	189.5M	0.0M
aih-tih-sc-934f55-R1_B23WHYVLT4_1	214.6M	0.0M	0.0M	17.2M	1.9M	187.2M	93.7M	93.6M	93.6M	93.6M	99.0M	88.3M	186.7M	0.0M
aih-tih-sc-973c73-R1_B23WHYVLT4_1	211.1M	0.0M	0.0M	13.4M	1.3M	190.2M	95.1M	95.0M	95.1M	95.1M	101.7M	88.5M	189.6M	0.0M
aih-tih-sc-976eb1-R1_B23WHYVLT4_1	161.0M	0.0M	0.0M	16.2M	4.6M	133.8M	67.0M	66.8M	66.9M	66.9M	89.1M	44.7M	133.3M	0.0M
aih-tih-sc-97c26d-R1_B23WHYVLT4_1	213.4M	0.0M	0.0M	16.1M	1.4M	188.7M	94.4M	94.3M	94.4M	94.3M	116.4M	72.3M	188.1M	0.0M
aih-tih-sc-97ecde-R1_B23WHYVLT4_1	215.9M	0.0M	0.0M	18.8M	1.8M	187.4M	93.8M	93.6M	93.7M	93.7M	113.5M	73.9M	186.8M	0.0M
aih-tih-sc-a03f86-R1_B23WHYVLT4_1	209.5M	0.0M	0.0M	11.8M	1.2M	190.6M	95.3M	95.3M	95.3M	95.3M	93.7M	96.9M	190.1M	0.0M
aih-tih-sc-a3a521-R1_B23WHYVLT4_1	209.9M	0.0M	0.0M	12.2M	1.2M	190.3M	95.2M	95.1M	95.1M	95.1M	97.4M	92.8M	189.7M	0.0M
aih-tih-sc-a49d10-R1_B23WHYVLT4_1	215.1M	0.0M	0.0M	17.6M	1.8M	187.8M	93.9M	93.8M	93.9M	93.9M	99.4M	88.4M	187.2M	0.0M
aih-tih-sc-a70f1e-R1_B23WHYVLT4_1	213.7M	0.0M	0.0M	16.4M	1.4M	188.5M	94.3M	94.2M	94.3M	94.2M	110.4M	78.0M	187.8M	0.0M
aih-tih-sc-ac6a71-R1_B23WHYVLT4_1	207.7M	0.0M	0.0M	10.0M	1.1M	191.6M	95.8M	95.7M	95.8M	95.8M	91.4M	100.1M	191.0M	0.0M
aih-tih-sc-b157e9-R1_B23WHYVLT4_1	213.6M	0.0M	0.0M	16.2M	2.2M	186.9M	93.5M	93.4M	93.5M	93.4M	101.8M	85.2M	186.3M	0.0M
aih-tih-sc-b845f7-R1_B23WHYVLT4_1	212.7M	0.0M	0.0M	15.3M	1.5M	188.7M	94.4M	94.3M	94.4M	94.3M	105.4M	83.3M	188.1M	0.0M
aih-tih-sc-b93682-R1_B23WHYVLT4_1	209.2M	0.0M	0.0M	11.6M	1.0M	191.2M	95.6M	95.6M	95.6M	95.6M	84.4M	106.8M	190.6M	0.0M
aih-tih-sc-be4b28-R1_B23WHYVLT4_1	210.1M	0.0M	0.0M	12.7M	1.2M	189.8M	94.9M	94.9M	94.9M	94.9M	101.3M	88.5M	189.2M	0.0M
aih-tih-sc-c04072-R1_B23WHYVLT4_1	208.5M	0.0M	0.0M	10.7M	1.4M	191.0M	95.6M	95.4M	95.5M	95.5M	86.6M	104.4M	190.3M	0.0M
aih-tih-sc-c55577-R1_B23WHYVLT4_1	237.0M	0.0M	0.0M	40.7M	2.2M	175.1M	87.6M	87.5M	87.5M	87.5M	106.2M	68.9M	174.5M	0.0M
aih-tih-sc-c5cb90-R1_B23WHYVLT4_1	225.3M	0.0M	0.0M	28.6M	1.6M	181.4M	90.8M	90.6M	90.7M	90.7M	102.4M	79.0M	180.7M	0.0M
aih-tih-sc-c63171-R1_B23WHYVLT4_1	218.3M	0.0M	0.0M	20.9M	1.5M	186.0M	93.1M	92.9M	93.0M	93.0M	100.7M	85.3M	185.4M	0.0M
aih-tih-sc-c7b68f-R1_B23WHYVLT4_1	208.9M	0.0M	0.0M	11.4M	1.1M	190.7M	95.4M	95.3M	95.4M	95.3M	96.5M	94.2M	190.0M	0.0M
aih-tih-sc-c9a01b-R1_B23WHYVLT4_1	208.5M	0.0M	0.0M	11.0M	1.3M	191.0M	95.5M	95.4M	95.5M	95.5M	92.4M	98.6M	190.4M	0.0M
aih-tih-sc-ce6733-R1_B23WHYVLT4_1	215.6M	0.0M	0.0M	18.3M	1.3M	187.0M	93.6M	93.4M	93.5M	93.5M	95.9M	91.1M	186.4M	0.0M
aih-tih-sc-d113b5-R1_B23WHYVLT4_1	210.7M	0.0M	0.0M	13.2M	1.4M	189.8M	95.0M	94.9M	95.0M	94.9M	97.4M	92.4M	189.1M	0.0M
aih-tih-sc-d12f1f-R1_B23WHYVLT4_1	208.6M	0.0M	0.0M	11.2M	1.0M	190.6M	95.3M	95.3M	95.3M	95.3M	98.3M	92.3M	189.9M	0.0M
aih-tih-sc-d2f1f4-R1_B23WHYVLT4_1	212.6M	0.0M	0.0M	15.3M	1.7M	188.7M	94.4M	94.3M	94.4M	94.3M	113.0M	75.7M	188.1M	0.0M
aih-tih-sc-d5506e-R1_B23WHYVLT4_1	210.0M	0.0M	0.0M	12.9M	1.4M	189.5M	94.8M	94.7M	94.8M	94.7M	97.9M	91.6M	188.9M	0.0M
aih-tih-sc-e9e54b-R1_B23WHYVLT4_1	215.6M	0.0M	0.0M	18.5M	1.7M	187.2M	93.7M	93.5M	93.6M	93.6M	109.9M	77.3M	186.6M	0.0M
aih-tih-sc-ea2fad-R1_B23WHYVLT4_1	210.6M	0.0M	0.0M	13.2M	1.0M	190.4M	95.2M	95.2M	95.2M	95.2M	105.5M	84.9M	189.8M	0.0M
aih-tih-sc-ea6ef3-R1_B23WHYVLT4_1	217.6M	0.0M	0.0M	20.4M	1.3M	185.6M	92.8M	92.7M	92.8M	92.8M	96.8M	88.8M	185.0M	0.0M
aih-tih-sc-f14fdc-R1_B23WHYVLT4_1	209.1M	0.0M	0.0M	11.6M	1.3M	190.6M	95.4M	95.3M	95.4M	95.3M	96.3M	94.3M	190.1M	0.0M
aih-tih-sc-f39d87-R1_B23WHYVLT4_1	216.4M	0.0M	0.0M	18.9M	1.5M	187.1M	93.6M	93.5M	93.6M	93.5M	108.7M	78.4M	186.6M	0.0M
aih-tih-sc-f4e5c4-R1_B23WHYVLT4_1	216.5M	0.0M	0.0M	19.8M	1.8M	186.4M	93.3M	93.1M	93.2M	93.2M	116.1M	70.4M	185.8M	0.0M
aih-tih-sc-f71b32-R1_B23WHYVLT4_1	213.0M	0.0M	0.0M	15.5M	1.1M	188.8M	94.4M	94.3M	94.4M	94.4M	97.6M	91.2M	188.2M	0.0M

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order. Table ID: table-rseqc_bam_stat

Sort	Column	Description	ID	Scale
\|\|	Total records	Total records	`total_records`	read_count
\|\|	QC failed	QC failed	`qc_failed`	read_count
\|\|	Duplicates	Optical/PCR duplicate	`optical_pcr_duplicate`	read_count
\|\|	Non primary hit	Non primary hit	`non_primary_hits`	read_count
\|\|	Unmapped	Unmapped reads	`unmapped_reads`	read_count
\|\|	Unique	mapq >= mapq_cut (unique)	`mapq_gte_mapq_cut_unique`	read_count
\|\|	Read-1	Read-1	`read_1`	read_count
\|\|	Read-2	Read-2	`read_2`	read_count
\|\|	+ve strand	Reads map to '+'	`reads_map_to_sense`	read_count
\|\|	-ve strand	Reads map to '-'	`reads_map_to_antisense`	read_count
\|\|	Non-splice reads	Non-splice reads	`non-splice_reads`	read_count
\|\|	Splice reads	Splice reads	`splice_reads`	read_count
\|\|	Proper pairs	Reads mapped in proper pairs	`reads_mapped_in_proper_pairs`	read_count
\|\|	Different chrom	Proper-paired reads map to different chrom	`proper-paired_reads_map_to_different_chrom`	read_count

STAR

STAR is an ultrafast universal RNA-seq aligner.DOI: 10.1093/bioinformatics/bts635.

Alignment Scores

Created with MultiQC

Gene Counts

Statistics from results generated using --quantMode GeneCounts. The three tabs show counts for unstranded RNA-seq, counts for the 1st read strand aligned with RNA and counts for the 2nd read strand aligned with RNA.

Created with MultiQC

fastp

fastp An ultra-fast all-in-one FASTQ preprocessor (QC, adapters, trimming, filtering, splitting...).DOI: 10.1093/bioinformatics/bty560.

Filtered Reads

Filtering statistics of sampled reads.

Created with MultiQC

Insert Sizes

Insert size estimation of sampled reads.

Created with MultiQC

Sequence Quality

Average sequencing quality over each base of all reads.

Created with MultiQC

GC Content

Average GC content over each base of all reads.

Created with MultiQC

N content

Average N content over each base of all reads.

Created with MultiQC

FastQC (raw)

FastQC (raw) This section of the report shows FastQC results before adapter trimming.

Sequence Counts

Sequence counts for each sample. Duplicate read counts are an estimate only.

This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

Created with MultiQC

Sequence Quality Histograms

The mean quality value across each base position in the read.

To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

Taken from the FastQC help:

The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

Created with MultiQC

Per Sequence Quality Scores

The number of reads with average quality scores. Shows if a subset of reads has poor quality.

From the FastQC help:

The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

Created with MultiQC

Per Base Sequence Content

The proportion of each base position for which each of the four normal DNA bases has been called.

To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

To see the data as a line plot, as in the original FastQC graph, click on a sample track.

From the FastQC help:

Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

Click a sample row to see a line plot for that dataset.

Rollover for sample name

Position: -

%T: -

%C: -

%A: -

%G: -

Per Sequence GC Content

The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

From the FastQC help:

This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

Created with MultiQC

Per Base N Content

The percentage of base calls at each position for which an N was called.

From the FastQC help:

If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

Created with MultiQC

Sequence Length Distribution

The distribution of fragment sizes (read lengths) found. See the FastQC help

Created with MultiQC

Sequence Duplication Levels

The relative level of duplication found for every sequence.

From the FastQC Help:

In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

Created with MultiQC

Overrepresented sequences by sample

The total amount of overrepresented sequences found in each library.

FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as overrepresented.

Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

From the FastQC Help:

A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

FastQC lists all the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

Created with MultiQC

Top overrepresented sequences

Top overrepresented sequences across all samples. The table shows 20 most overrepresented sequences across all samples, ranked by the number of samples they occur in.

Showing ²⁰/₂₀ rows and ³/₃ columns.

Overrepresented sequence	Samples	Occurrences	% of all reads
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	192	286088497	0.3648%
GCCAGTTTTGCCATCAGGACCAGGGCTGCCAGGGCTTCCAGTCAGACCCT	6	1295117	0.0017%
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA	4	9573	0.0000%
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC	4	7530	0.0000%
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT	3	3946	0.0000%
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG	3	3020	0.0000%
ATTTCCACGAGCACCAGCAGGGCCAGGGGCTCCAGGGCGACCTCTCTCAC	2	318115	0.0004%
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGG	2	9574	0.0000%
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGG	2	8115	0.0000%
GGCACAGTGTACAGCACGCTGAAGACGCCAATGCGCACCATGAGCTTCTC	2	83	0.0000%
CTGGAAGACCGAGTGGTGTGTAATGACAAGTTCGCCGAGGACGGGGCACG	2	78	0.0000%
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCTGGT	1	445414	0.0006%
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCTGGG	1	224619	0.0003%
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCTAGT	1	149788	0.0002%
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCTAGG	1	46637	0.0001%
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCTGGTG	1	4572	0.0000%
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCTGGGG	1	3627	0.0000%
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCTGGTA	1	2896	0.0000%
CGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCTGGTGTG	1	2861	0.0000%
CGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCTGGTATG	1	2067	0.0000%

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order. Table ID: table-fastqc_top_overrepresented_sequences_table

Sort	Group	Column	Description	ID	Scale
\|\|	FastQC (raw)	Samples	Number of samples where this sequence is overrepresented	`samples`	None
\|\|	FastQC (raw)	Occurrences	Total number of occurrences of the sequence (among the samples where the sequence is overrepresented)	`total_count`	None
\|\|	FastQC (raw)	% of all reads	Total number of occurrences as the percentage of all reads (among samples where the sequence is overrepresented)	`total_percent`	None

Adapter Content

The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

Note that only samples with ≥ 0.1% adapter contamination are shown.

There may be several lines per sample, as one is shown for each adapter detected in the file.

From the FastQC Help:

The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

Created with MultiQC

Status Checks

Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

Min:

Max:

Created with MultiQC

FastQC (trimmed)

FastQC (trimmed) This section of the report shows FastQC results after adapter trimming.

Sequence Counts

Sequence counts for each sample. Duplicate read counts are an estimate only.

This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

Created with MultiQC

Sequence Quality Histograms

The mean quality value across each base position in the read.

To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

Taken from the FastQC help:

Created with MultiQC

Per Sequence Quality Scores

The number of reads with average quality scores. Shows if a subset of reads has poor quality.

From the FastQC help:

Created with MultiQC

Per Base Sequence Content

The proportion of each base position for which each of the four normal DNA bases has been called.

To see the data as a line plot, as in the original FastQC graph, click on a sample track.

From the FastQC help:

Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

Click a sample row to see a line plot for that dataset.

Rollover for sample name

Position: -

%T: -

%C: -

%A: -

%G: -

Per Sequence GC Content

The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

From the FastQC help:

This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

Created with MultiQC

Per Base N Content

The percentage of base calls at each position for which an N was called.

From the FastQC help:

Created with MultiQC

Sequence Length Distribution

The distribution of fragment sizes (read lengths) found. See the FastQC help

Created with MultiQC

Sequence Duplication Levels

The relative level of duplication found for every sequence.

From the FastQC Help:

The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

Created with MultiQC

Overrepresented sequences by sample

The total amount of overrepresented sequences found in each library.

From the FastQC Help:

Created with MultiQC

Top overrepresented sequences

Top overrepresented sequences across all samples. The table shows 20 most overrepresented sequences across all samples, ranked by the number of samples they occur in.

Showing ²⁰/₂₀ rows and ³/₃ columns.

Overrepresented sequence	Samples	Occurrences	% of all reads
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	96	60335108	0.3242%
GCCAGTTTTGCCATCAGGACCAGGGCTGCCAGGGCTTCCAGTCAGACCCT	3	324718	0.0017%
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA	2	4740	0.0000%
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC	2	3760	0.0000%
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG	2	1832	0.0000%
GGCACAGTGTACAGCACGCTGAAGACGCCAATGCGCACCATGAGCTTCTC	2	83	0.0000%
CTGGAAGACCGAGTGGTGTGTAATGACAAGTTCGCCGAGGACGGGGCACG	2	78	0.0000%
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT	2	1986	0.0000%
GCTGCCGAGTGTTACAGCCCCCCCGGCAGC	1	241	0.0000%
GCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC	1	140	0.0000%
TTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC	1	70	0.0000%
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGG	1	62	0.0000%
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGG	1	58	0.0000%
TCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC	1	46	0.0000%
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCC	1	45	0.0000%
GCGGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA	1	38	0.0000%
CTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT	1	38	0.0000%
GACTGGAGTTCAGACGTGTGCTCTCCGATC	1	35	0.0000%
GCGGGGGAGAGAGAGAGAGAGAGGGCGC	1	35	0.0000%
GATCACCCTCCTCAAGGCTGCCTGCCTGGACATCCTGATCCTGCGGATCT	1	32	0.0000%

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order. Table ID: table-fastqc_top_overrepresented_sequences_table

Sort	Group	Column	Description	ID	Scale
\|\|	FastQC (trimmed)	Samples	Number of samples where this sequence is overrepresented	`samples`	None
\|\|	FastQC (trimmed)	Occurrences	Total number of occurrences of the sequence (among the samples where the sequence is overrepresented)	`total_count`	None
\|\|	FastQC (trimmed)	% of all reads	Total number of occurrences as the percentage of all reads (among samples where the sequence is overrepresented)	`total_percent`	None

Adapter Content

The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

Note that only samples with ≥ 0.1% adapter contamination are shown.

There may be several lines per sample, as one is shown for each adapter detected in the file.

From the FastQC Help:

Created with MultiQC

Status Checks

Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

Min:

Max:

Created with MultiQC

Software Versions

Software Versions lists versions of software tools extracted from file contents.

Group	Software	Version
AGAT_CONVERTSPGFF2TSV	agat	`1.2.0`
ARRIBA	arriba	`2.4.0`
ARRIBA_FILTER_SAME_GENE	awk	`filter same-gene (gene1==gene2); exclude IGHA1 in gene1 or gene2`
ARRIBA_VISUALISATION	arriba	`2.4.0`
CANCER_INTRONS_TO_VCF	python	`3.11.0`
CONTAMINATION_SKIPPED_LOW_READS	contamination_skipped_low_reads	`1.0.0`
CTATSPLICING_STARTOCANCERINTRONS	ctat-splicing	`0.0.3`
EXTRACT_VAF_FEATURES	extract_vaf_features	`1.0.0`
	python	`3.11.14`
FASTQC	fastqc	`0.12.1`
FASTQC_FOR_FASTP	fastqc	`0.12.1`
FUSIONINSPECTOR	STAR-Fusion	`1.12.0`
FUSIONREPORT	fusion_report	`2.1.5`
	fusion_report DB retrieval	`null`
GATK4_MARKDUPLICATES	gatk4	`4.4.0.0`
	samtools	`1.17`
GTF2BED	perl	`5.26.2`
KALLISTO_QUANT	kallisto	`0.51.1`
KALLISTO_TO_TX2GENE_QC	expression_qc_and_signatures	`1.0.0`
	python	`3.11.0`
NGSCheckMATE	ngscheckmate	`1.0.0`
PICARD_COLLECTINSERTSIZEMETRICS	picard	`3.1.0`
PICARD_COLLECTRNASEQMETRICS	picard	`null`
PREDICT_CONTAMINATION	predict_contamination	`1.0.0`
	python	`3.11.14`
PRESEQ_LCEXTRAP	preseq	`3.2.0`
PRESEQ_TO_MULTIQC	preseq_to_multiqc	`1.0.0`
	python	`3.11.14`
RSEQC_BAMSTAT	rseqc	`5.0.2`
RSEQC_INFEREXPERIMENT	rseqc	`5.0.2`
RSEQC_INNERDISTANCE	rseqc	`5.0.2`
RSEQC_JUNCTIONANNOTATION	rseqc	`5.0.2`
RSEQC_JUNCTIONSATURATION	rseqc	`5.0.2`
RSEQC_READDISTRIBUTION	rseqc	`5.0.2`
RSEQC_READDUPLICATION	rseqc	`5.0.2`
SAMTOOLS_INDEX_ALIGNMENT_CRAM	samtools	`1.17`
SAMTOOLS_INDEX_STAR_ALIGNMENT	samtools	`1.17`
SAMTOOLS_VIEW_ALIGNMENT_CRAM	samtools	`1.17`
SEQTOOL_TO_MULTIQC	python	`3.11.14`
	seqtool_to_multiqc	`1.0.0`
STARFUSION	STAR-Fusion	`1.12.0`
STARFUSION_FILTER	filter_starfusion_main.py	`1.0`
	numpy	`2.4.1`
	pandas	`3.0.0`
	pyyaml	`6.0.3`
STAR_ALIGNMENT	gawk	`5.1.0`
	samtools	`1.16.1`
	star	`2.7.10a0`
VCF_COLLECT	HGNC DB retrieval	`2025-01-22/23:32`
	python	`3.11.0`
Workflow	Nextflow	`25.10.2`
	nf-core/rnafusion	`3.0.2`
fastp	fastp	`0.23.4`

nf-core/rnafusion Methods Description

Suggested text and references to use when describing pipeline usage within the methods section of a publication.

Methods

Data was processed using nf-core/rnafusion v3.0.2 of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

The pipeline was executed with Nextflow v25.10.2 (Di Tommaso et al., 2017) with the following command:

nextflow run /code/main.nf -output-dir 's3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/dev_rna_exp0018/B23WHYVLT4/nfcore_rnafusion__fd1be10--20260514-142812' --project-dir /Users/anaik/Documents/Altera/rnafusion -profile docker,eks,dev_altera_demux_test_singleSTAR,dev_demux -name dev_rna_exp0018_2-fd1be10 --rundir 's3://natera-labdata-rnd-dev-s3/illumina/20260501_LH00995_0036_B23WHYVLT4/' --bclconvert_samplesheet 's3://natera-labdata-rnd-dev-s3/illumina/20260501_LH00995_0036_B23WHYVLT4/bclconvert_samplesheet_corrected.csv' --flowcell_id 20260501_LH00995_0036_B23WHYVLT4 --outdir 's3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/dev_rna_exp0018/B23WHYVLT4/nfcore_rnafusion__fd1be10--20260514-142812' -params-file 's3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/dev_rna_exp0018/B23WHYVLT4/nfcore_rnafusion__fd1be10--20260514-142812/_info/params_2026-05-14t21-29-36z.json'

References

Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. doi: 10.1038/nbt.3820
Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. doi: 10.1038/s41587-020-0439-x
Grüning, B., Dale, R., Sjödin, A., Chapman, B. A., Rowe, J., Tomkins-Tinch, C. H., Valieris, R., Köster, J., & Bioconda Team. (2018). Bioconda: sustainable and comprehensive software distribution for the life sciences. Nature Methods, 15(7), 475–476. doi: 10.1038/s41592-018-0046-7
da Veiga Leprevost, F., Grüning, B. A., Alves Aflitos, S., Röst, H. L., Uszkoreit, J., Barsnes, H., Vaudel, M., Moreno, P., Gatto, L., Weber, J., Bai, M., Jimenez, R. C., Sachsenberg, T., Pfeuffer, J., Vera Alvarez, R., Griss, J., Nesvizhskii, A. I., & Perez-Riverol, Y. (2017). BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics (Oxford, England), 33(16), 2580–2582. doi: 10.1093/bioinformatics/btx192

Notes:

If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
The command above does not include parameters contained in any configs or profiles that may have been used. Ensure the config file is also uploaded with your publication!
You should also cite all software used within this run. Check the "Software Versions" of this report to get version information.

nf-core/rnafusion Workflow Summary

- this information is collected when the pipeline is started.

Core Nextflow options

runName: dev_rna_exp0018_2-fd1be10
containerEngine: docker
launchDir: /code
workDir: /natera-rnd-pltf-dev-nextflow-scratch-01/work
projectDir: /code
userName: nextflow
profile: docker,eks,dev_altera_demux_test_singleSTAR,dev_demux
configFiles: N/A

Input/output options

outdir: s3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/dev_rna_exp0018/B23WHYVLT4/nfcore_rnafusion__fd1be10--20260514-142812
rundir: s3://natera-labdata-rnd-dev-s3/illumina/20260501_LH00995_0036_B23WHYVLT4/
flowcell_id: 20260501_LH00995_0036_B23WHYVLT4
strand: reverse
bclconvert_samplesheet: s3://natera-labdata-rnd-dev-s3/illumina/20260501_LH00995_0036_B23WHYVLT4/bclconvert_samplesheet_corrected.csv
infer_sample_type_map: true
sample_type_map_positive_substring: HD789
genomes_base: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38
read_length: 150
arriba: true
arriba_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/
arriba_ref_blacklist: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz
arriba_ref_cytobands: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv
arriba_ref_known_fusions: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz
arriba_ref_protein_domains: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3
ensembl_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/
fusioncatcher_limitSjdbInsertNsj: 2000000
fusioncatcher_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/fusioncatcher/human_v102/
fusioninspector_limitSjdbInsertNsj: 1000000
fusionreport_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/fusion_report_db/
hgnc_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/hgnc/hgnc_complete_set.txt
hgnc_date: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/hgnc/HGNC-DB-timestamp.txt
starfusion: true
starfusion_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/
starindex_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/star/
starindex_ensembl_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/star/
tools_cutoff: 1

Read trimming options

fastp_trim: true
fastp_reads_to_process: 100000000
adapter_fasta: []

Alignment compression options

cram: starfusion,arriba

Reference genome options

genome: GRCh38
fasta: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.all.fa
fai: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.all.fa.fai
gtf: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.gtf
chrgtf: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.chr.gtf
transcript: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.cdna.all.fa.gz
refflat: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.chr.gtf.refflat
rrna_intervals: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.interval_list

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Software Versions

nf-core/rnafusion Methods Description

Methods

References

Notes:

nf-core/rnafusion Workflow Summary

v1.21

Highlight Samples

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Sequence Counts

Sequence Quality Histograms

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Per Base Sequence Content

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Sequence Counts

Sequence Quality Histograms

Per Sequence Quality Scores

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