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BCL CONVERT QC REPORT - DETAILED
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1. % UNDETERMINED PER LANE
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%_Undetermined Undetermined_Reads Total_Reads
Lane
1 7.32 286061952 3905493663
2 7.42 290628407 3916062329
3 7.45 291127112 3906434199
4 7.40 290993237 3930795132
5 7.36 286383528 3893661451
6 7.33 286651972 3909662712
7 7.35 290048155 3944072934
8 7.49 296699294 3959672609
2. SAMPLES WITH LOW READS (NON-NTC)
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Found 1 samples with low reads:
SampleID Index # Reads Lane(s)
20 aih-tih-sc-331e97-R1_A23WJ53LT4_1 TTCTCTTAGC-TCCTCGAGCT 841936 1,2,3,4,5,6,7,8
3. NTC SAMPLES WITH HIGH READS
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No NTC samples found with high reads
4. TOP UNKNOWN BARCODES
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Found 90 significant unknown barcodes:
Lane index index2 # Reads % of Unknown Barcodes % of All Reads Percentage_of_Lane Percentage_of_Total
0 1 TATCCGCGTC GGGGGGGGGG 6248792 0.021844 0.001600 0.160000 0.019922
1 1 AGATTAGCGT GGGGGGGGGG 6198332 0.021668 0.001587 0.158708 0.019761
2 1 CCAGTCGTCA GGGGGGGGGG 5183865 0.018121 0.001327 0.132733 0.016527
3 1 CCTAAGCTGA GGGGGGGGGG 4882627 0.017068 0.001250 0.125019 0.015567
4 1 ATGACAGCAC GGGGGGGGGG 4528081 0.015829 0.001159 0.115941 0.014436
5 1 TAACTTGGTC GGGGGGGGGG 3190033 0.011152 0.000817 0.081681 0.010170
6 1 CAACTGCGCA GGGGGGGGGG 2777064 0.009708 0.000711 0.071107 0.008854
7 1 GAGCAGCTTG GGGGGGGGGG 2611011 0.009127 0.000669 0.066855 0.008324
8 1 GCCACCTAGT GGGGGGGGGG 2346520 0.008203 0.000601 0.060083 0.007481
9 1 TCCAACTGAA GGGGGGGGGG 2242789 0.007840 0.000574 0.057427 0.007150
10 1 CGATGTCTTG GGGGGGGGGG 2124212 0.007426 0.000544 0.054390 0.006772
11 2 TATCCGCGTC GGGGGGGGGG 6294587 0.021659 0.001607 0.160738 0.020068
12 2 AGATTAGCGT GGGGGGGGGG 6261236 0.021544 0.001599 0.159886 0.019962
13 2 CCAGTCGTCA GGGGGGGGGG 5304961 0.018253 0.001355 0.135467 0.016913
14 2 CCTAAGCTGA GGGGGGGGGG 4986166 0.017156 0.001273 0.127326 0.015897
15 2 ATGACAGCAC GGGGGGGGGG 4600080 0.015828 0.001175 0.117467 0.014666
16 2 TAACTTGGTC GGGGGGGGGG 3217695 0.011072 0.000822 0.082167 0.010259
17 2 CAACTGCGCA GGGGGGGGGG 2763315 0.009508 0.000706 0.070564 0.008810
18 2 GAGCAGCTTG GGGGGGGGGG 2639715 0.009083 0.000674 0.067407 0.008416
19 2 GCCACCTAGT GGGGGGGGGG 2410853 0.008295 0.000616 0.061563 0.007686
20 2 AACGAAGATC GGGGGGGGGG 2260425 0.007778 0.000577 0.057722 0.007207
21 2 TCCAACTGAA GGGGGGGGGG 2233491 0.007685 0.000570 0.057034 0.007121
22 2 CGATGTCTTG GGGGGGGGGG 1999593 0.006880 0.000511 0.051061 0.006375
23 3 TATCCGCGTC GGGGGGGGGG 6203798 0.021310 0.001588 0.158810 0.019779
24 3 AGATTAGCGT GGGGGGGGGG 6099594 0.020952 0.001561 0.156142 0.019447
25 3 CCAGTCGTCA GGGGGGGGGG 5190308 0.017828 0.001329 0.132866 0.016548
26 3 CCTAAGCTGA GGGGGGGGGG 4911242 0.016870 0.001257 0.125722 0.015658
27 3 ATGACAGCAC GGGGGGGGGG 4514485 0.015507 0.001156 0.115565 0.014393
28 3 TAACTTGGTC GGGGGGGGGG 3181419 0.010928 0.000814 0.081440 0.010143
29 3 CAACTGCGCA GGGGGGGGGG 2738832 0.009408 0.000701 0.070111 0.008732
30 3 GAGCAGCTTG GGGGGGGGGG 2602604 0.008940 0.000666 0.066624 0.008298
31 3 GCCACCTAGT GGGGGGGGGG 2397360 0.008235 0.000614 0.061370 0.007643
32 3 AACGAAGATC GGGGGGGGGG 2312980 0.007945 0.000592 0.059209 0.007374
33 3 TCCAACTGAA GGGGGGGGGG 2200254 0.007558 0.000563 0.056324 0.007015
34 4 TATCCGCGTC GGGGGGGGGG 6127227 0.021056 0.001559 0.155878 0.019535
35 4 AGATTAGCGT GGGGGGGGGG 6066087 0.020846 0.001543 0.154322 0.019340
36 4 CCAGTCGTCA GGGGGGGGGG 5143620 0.017676 0.001309 0.130854 0.016399
37 4 CCTAAGCTGA GGGGGGGGGG 4895816 0.016825 0.001246 0.124550 0.015609
38 4 ATGACAGCAC GGGGGGGGGG 4476349 0.015383 0.001139 0.113879 0.014271
39 4 TAACTTGGTC GGGGGGGGGG 3178792 0.010924 0.000809 0.080869 0.010135
40 4 CAACTGCGCA GGGGGGGGGG 2688384 0.009239 0.000684 0.068393 0.008571
41 4 GAGCAGCTTG GGGGGGGGGG 2575859 0.008852 0.000655 0.065530 0.008212
42 4 GCCACCTAGT GGGGGGGGGG 2348173 0.008070 0.000597 0.059738 0.007486
43 4 AACGAAGATC GGGGGGGGGG 2293098 0.007880 0.000583 0.058337 0.007311
44 4 TCCAACTGAA GGGGGGGGGG 2165764 0.007443 0.000551 0.055097 0.006905
45 5 TATCCGCGTC GGGGGGGGGG 6060952 0.021164 0.001557 0.155662 0.019323
46 5 AGATTAGCGT GGGGGGGGGG 6006577 0.020974 0.001543 0.154266 0.019150
47 5 CCAGTCGTCA GGGGGGGGGG 5090540 0.017775 0.001307 0.130739 0.016230
48 5 CCTAAGCTGA GGGGGGGGGG 4839385 0.016898 0.001243 0.124289 0.015429
49 5 ATGACAGCAC GGGGGGGGGG 4437643 0.015495 0.001140 0.113971 0.014148
50 5 TAACTTGGTC GGGGGGGGGG 3145742 0.010984 0.000808 0.080791 0.010029
51 5 CAACTGCGCA GGGGGGGGGG 2628735 0.009179 0.000675 0.067513 0.008381
52 5 GAGCAGCTTG GGGGGGGGGG 2554600 0.008920 0.000656 0.065609 0.008145
53 5 GCCACCTAGT GGGGGGGGGG 2314163 0.008081 0.000594 0.059434 0.007378
54 5 AACGAAGATC GGGGGGGGGG 2159894 0.007542 0.000555 0.055472 0.006886
55 5 TCCAACTGAA GGGGGGGGGG 2155966 0.007528 0.000554 0.055371 0.006874
56 6 TATCCGCGTC GGGGGGGGGG 6191964 0.021601 0.001584 0.158376 0.019741
57 6 AGATTAGCGT GGGGGGGGGG 6146672 0.021443 0.001572 0.157217 0.019597
58 6 CCAGTCGTCA GGGGGGGGGG 5209309 0.018173 0.001332 0.133242 0.016608
59 6 CCTAAGCTGA GGGGGGGGGG 4930048 0.017199 0.001261 0.126099 0.015718
60 6 ATGACAGCAC GGGGGGGGGG 4525100 0.015786 0.001157 0.115741 0.014427
61 6 TAACTTGGTC GGGGGGGGGG 3214419 0.011214 0.000822 0.082217 0.010248
62 6 CAACTGCGCA GGGGGGGGGG 2694420 0.009400 0.000689 0.068917 0.008590
63 6 GAGCAGCTTG GGGGGGGGGG 2604407 0.009086 0.000666 0.066615 0.008303
64 6 GCCACCTAGT GGGGGGGGGG 2357386 0.008224 0.000603 0.060296 0.007516
65 6 TCCAACTGAA GGGGGGGGGG 2204123 0.007689 0.000564 0.056376 0.007027
66 6 AACGAAGATC GGGGGGGGGG 2194101 0.007654 0.000561 0.056120 0.006995
67 7 TATCCGCGTC GGGGGGGGGG 6350065 0.021893 0.001610 0.161003 0.020245
68 7 AGATTAGCGT GGGGGGGGGG 6292441 0.021694 0.001595 0.159542 0.020061
69 7 CCAGTCGTCA GGGGGGGGGG 5315451 0.018326 0.001348 0.134771 0.016947
70 7 CCTAAGCTGA GGGGGGGGGG 5050472 0.017413 0.001281 0.128052 0.016102
71 7 ATGACAGCAC GGGGGGGGGG 4604654 0.015875 0.001167 0.116749 0.014680
72 7 TAACTTGGTC GGGGGGGGGG 3276888 0.011298 0.000831 0.083084 0.010447
73 7 CAACTGCGCA GGGGGGGGGG 2815458 0.009707 0.000714 0.071385 0.008976
74 7 GAGCAGCTTG GGGGGGGGGG 2671755 0.009211 0.000677 0.067741 0.008518
75 7 GCCACCTAGT GGGGGGGGGG 2436662 0.008401 0.000618 0.061780 0.007769
76 7 AACGAAGATC GGGGGGGGGG 2259548 0.007790 0.000573 0.057290 0.007204
77 7 TCCAACTGAA GGGGGGGGGG 2259012 0.007788 0.000573 0.057276 0.007202
78 7 CGATGTCTTG GGGGGGGGGG 2014590 0.006946 0.000511 0.051079 0.006423
79 8 TATCCGCGTC GGGGGGGGGG 6491593 0.021879 0.001639 0.163943 0.020696
80 8 AGATTAGCGT GGGGGGGGGG 6460951 0.021776 0.001632 0.163169 0.020599
81 8 CCAGTCGTCA GGGGGGGGGG 5446110 0.018356 0.001375 0.137539 0.017363
82 8 CCTAAGCTGA GGGGGGGGGG 5139363 0.017322 0.001298 0.129793 0.016385
83 8 ATGACAGCAC GGGGGGGGGG 4730777 0.015945 0.001195 0.119474 0.015083
84 8 TAACTTGGTC GGGGGGGGGG 3313421 0.011168 0.000837 0.083679 0.010564
85 8 CAACTGCGCA GGGGGGGGGG 2822091 0.009512 0.000713 0.071271 0.008997
86 8 GAGCAGCTTG GGGGGGGGGG 2740501 0.009237 0.000692 0.069210 0.008737
87 8 GCCACCTAGT GGGGGGGGGG 2457672 0.008283 0.000621 0.062068 0.007836
88 8 AACGAAGATC GGGGGGGGGG 2324201 0.007834 0.000587 0.058697 0.007410
89 8 TCCAACTGAA GGGGGGGGGG 2283993 0.007698 0.000577 0.057681 0.007282
5. BARCODES WITH HIGH MISMATCH RATES
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No barcodes found with high mismatch rates
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FINAL STATUS CHECK
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Overall % Undetermined: 7.39%
Status: WARNING
Reason(s):
Overall undetermined 7.39% >= 5.0%