FastQCFastQC Report
Mon 8 Jun 2026
NTC_0001_0001_A23WJ53LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_A23WJ53LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57079
Total Bases8.6 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC75

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTGGG4771883.59992291385623TruSeq Adapter, Index 27 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTGGGGGG18793.291928730356173TruSeq Adapter, Index 27 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTGGT9511.666111880025929TruSeq Adapter, Index 27 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTGGGG7531.3192242330804673TruSeq Adapter, Index 27 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTGG5100.8934984845564918TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTAGG4770.8356838767322483TruSeq Adapter, Index 27 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTGGGGTG2320.40645421258256104TruSeq Adapter, Index 27 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATGTGGG2040.3573993938225968TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATTTGGG1870.32761611100404703TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCGGGG1740.30484065943692074TruSeq Adapter, Index 27 (97% over 37bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1140.1997231906655688No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTGGGGG800.14015662502846932TruSeq Adapter, Index 27 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTAGT770.13490075158990172TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATATGGG610.10686942658420787TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTGGGG600.10511746877135197TruSeq Adapter, Index 15 (96% over 31bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCTTACTGATCTGGG600.10511746877135197TruSeq Adapter, Index 15 (97% over 36bp)
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG590.10336551095849612No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC580.10161355314564026No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)