FastQCFastQC Report
Mon 8 Jun 2026
aih-tih-sc-331e97-R1_A23WJ53LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameaih-tih-sc-331e97-R1_A23WJ53LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841936
Total Bases127.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTTAGCATCTAGG542606.444670378746128TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTTAGCATCTAGT502615.969693658425344TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTTAGCATCTGGG93291.108041466334733TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTTAGCATCTGGT45640.542083958875734TruSeq Adapter, Index 1 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTTAGCATCTAGTGTG10920.12970106991505292TruSeq Adapter, Index 1 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTTAGCATCTAGGGGG10460.12423747173181808TruSeq Adapter, Index 1 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTCTCTTAGCATCTAGGGTG9280.11022215465308527TruSeq Adapter, Index 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)