Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 841936 total bases: 127132336 Q20 bases: 117201575(92.1886%) Q30 bases: 104977658(82.5735%) Read2 before filtering: total reads: 841936 total bases: 127132336 Q20 bases: 122613616(96.4457%) Q30 bases: 111939544(88.0496%) Read1 after filtering: total reads: 698690 total bases: 63987076 Q20 bases: 63675603(99.5132%) Q30 bases: 62350297(97.442%) Read2 after filtering: total reads: 698690 total bases: 64232289 Q20 bases: 63776943(99.2911%) Q30 bases: 62254457(96.9208%) Filtering result: reads passed filter: 1397380 reads failed due to low quality: 34782 reads failed due to too many N: 128 reads failed due to too short: 251582 reads with adapter trimmed: 1474849 bases trimmed due to adapters: 88008735 Duplication rate: 27.3956% Insert size peak (evaluated by paired-end reads): 86 JSON report: aih-tih-sc-331e97-R1_A23WJ53LT4_1.fastp.json HTML report: aih-tih-sc-331e97-R1_A23WJ53LT4_1.fastp.html fastp --in1 aih-tih-sc-331e97-R1_A23WJ53LT4_1_1.fastq.gz --in2 aih-tih-sc-331e97-R1_A23WJ53LT4_1_2.fastq.gz --out1 aih-tih-sc-331e97-R1_A23WJ53LT4_1_1.fastp.fastq.gz --out2 aih-tih-sc-331e97-R1_A23WJ53LT4_1_2.fastp.fastq.gz --json aih-tih-sc-331e97-R1_A23WJ53LT4_1.fastp.json --html aih-tih-sc-331e97-R1_A23WJ53LT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 6 seconds