[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2482ef-R1_A23WJ53LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (1443897) splicing reads. 127482 splicing junctions. 118301 known splicing junctions. 9181 novel splicing junctions.
sampling 10% (2887794) splicing reads. 144926 splicing junctions. 131722 known splicing junctions. 13204 novel splicing junctions.
sampling 15% (4331691) splicing reads. 152818 splicing junctions. 137265 known splicing junctions. 15553 novel splicing junctions.
sampling 20% (5775588) splicing reads. 157328 splicing junctions. 140246 known splicing junctions. 17082 novel splicing junctions.
sampling 25% (7219485) splicing reads. 160222 splicing junctions. 142083 known splicing junctions. 18139 novel splicing junctions.
sampling 30% (8663382) splicing reads. 162307 splicing junctions. 143284 known splicing junctions. 19023 novel splicing junctions.
sampling 35% (10107279) splicing reads. 163796 splicing junctions. 144152 known splicing junctions. 19644 novel splicing junctions.
sampling 40% (11551176) splicing reads. 164965 splicing junctions. 144813 known splicing junctions. 20152 novel splicing junctions.
sampling 45% (12995073) splicing reads. 165918 splicing junctions. 145337 known splicing junctions. 20581 novel splicing junctions.
sampling 50% (14438971) splicing reads. 166686 splicing junctions. 145770 known splicing junctions. 20916 novel splicing junctions.
sampling 55% (15882868) splicing reads. 167307 splicing junctions. 146096 known splicing junctions. 21211 novel splicing junctions.
sampling 60% (17326765) splicing reads. 167785 splicing junctions. 146358 known splicing junctions. 21427 novel splicing junctions.
sampling 65% (18770662) splicing reads. 168210 splicing junctions. 146575 known splicing junctions. 21635 novel splicing junctions.
sampling 70% (20214559) splicing reads. 168557 splicing junctions. 146754 known splicing junctions. 21803 novel splicing junctions.
sampling 75% (21658456) splicing reads. 168828 splicing junctions. 146889 known splicing junctions. 21939 novel splicing junctions.
sampling 80% (23102353) splicing reads. 169060 splicing junctions. 147000 known splicing junctions. 22060 novel splicing junctions.
sampling 85% (24546250) splicing reads. 169228 splicing junctions. 147078 known splicing junctions. 22150 novel splicing junctions.
sampling 90% (25990147) splicing reads. 169353 splicing junctions. 147145 known splicing junctions. 22208 novel splicing junctions.
sampling 95% (27434044) splicing reads. 169464 splicing junctions. 147200 known splicing junctions. 22264 novel splicing junctions.
sampling 100% (28877942) splicing reads. 169525 splicing junctions. 147231 known splicing junctions. 22294 novel splicing junctions.