[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-00d05f-R1_A23WJ53LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (12439651) splicing reads. 243898 splicing junctions. 184394 known splicing junctions. 59504 novel splicing junctions.
sampling 10% (24879303) splicing reads. 284553 splicing junctions. 195415 known splicing junctions. 89138 novel splicing junctions.
sampling 15% (37318955) splicing reads. 309950 splicing junctions. 200796 known splicing junctions. 109154 novel splicing junctions.
sampling 20% (49758607) splicing reads. 327580 splicing junctions. 204079 known splicing junctions. 123501 novel splicing junctions.
sampling 25% (62198259) splicing reads. 340629 splicing junctions. 206377 known splicing junctions. 134252 novel splicing junctions.
sampling 30% (74637910) splicing reads. 350808 splicing junctions. 208061 known splicing junctions. 142747 novel splicing junctions.
sampling 35% (87077562) splicing reads. 358934 splicing junctions. 209310 known splicing junctions. 149624 novel splicing junctions.
sampling 40% (99517214) splicing reads. 365598 splicing junctions. 210340 known splicing junctions. 155258 novel splicing junctions.
sampling 45% (111956866) splicing reads. 371021 splicing junctions. 211171 known splicing junctions. 159850 novel splicing junctions.
sampling 50% (124396518) splicing reads. 375558 splicing junctions. 211773 known splicing junctions. 163785 novel splicing junctions.
sampling 55% (136836169) splicing reads. 379425 splicing junctions. 212328 known splicing junctions. 167097 novel splicing junctions.
sampling 60% (149275821) splicing reads. 382601 splicing junctions. 212778 known splicing junctions. 169823 novel splicing junctions.
sampling 65% (161715473) splicing reads. 385316 splicing junctions. 213156 known splicing junctions. 172160 novel splicing junctions.
sampling 70% (174155125) splicing reads. 387608 splicing junctions. 213466 known splicing junctions. 174142 novel splicing junctions.
sampling 75% (186594777) splicing reads. 389605 splicing junctions. 213745 known splicing junctions. 175860 novel splicing junctions.
sampling 80% (199034428) splicing reads. 391264 splicing junctions. 213969 known splicing junctions. 177295 novel splicing junctions.
sampling 85% (211474080) splicing reads. 392583 splicing junctions. 214134 known splicing junctions. 178449 novel splicing junctions.
sampling 90% (223913732) splicing reads. 393644 splicing junctions. 214269 known splicing junctions. 179375 novel splicing junctions.
sampling 95% (236353384) splicing reads. 394506 splicing junctions. 214375 known splicing junctions. 180131 novel splicing junctions.
sampling 100% (248793036) splicing reads. 395147 splicing junctions. 214474 known splicing junctions. 180673 novel splicing junctions.