[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-06a169-R1_A23WJ53LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (14037640) splicing reads. 245202 splicing junctions. 179239 known splicing junctions. 65963 novel splicing junctions.
sampling 10% (28075280) splicing reads. 287092 splicing junctions. 189934 known splicing junctions. 97158 novel splicing junctions.
sampling 15% (42112921) splicing reads. 312355 splicing junctions. 195053 known splicing junctions. 117302 novel splicing junctions.
sampling 20% (56150561) splicing reads. 329959 splicing junctions. 198313 known splicing junctions. 131646 novel splicing junctions.
sampling 25% (70188202) splicing reads. 342474 splicing junctions. 200369 known splicing junctions. 142105 novel splicing junctions.
sampling 30% (84225842) splicing reads. 352245 splicing junctions. 201892 known splicing junctions. 150353 novel splicing junctions.
sampling 35% (98263482) splicing reads. 359882 splicing junctions. 203069 known splicing junctions. 156813 novel splicing junctions.
sampling 40% (112301123) splicing reads. 366131 splicing junctions. 203932 known splicing junctions. 162199 novel splicing junctions.
sampling 45% (126338763) splicing reads. 371265 splicing junctions. 204638 known splicing junctions. 166627 novel splicing junctions.
sampling 50% (140376404) splicing reads. 375473 splicing junctions. 205200 known splicing junctions. 170273 novel splicing junctions.
sampling 55% (154414044) splicing reads. 379010 splicing junctions. 205665 known splicing junctions. 173345 novel splicing junctions.
sampling 60% (168451684) splicing reads. 382010 splicing junctions. 206089 known splicing junctions. 175921 novel splicing junctions.
sampling 65% (182489325) splicing reads. 384481 splicing junctions. 206393 known splicing junctions. 178088 novel splicing junctions.
sampling 70% (196526965) splicing reads. 386582 splicing junctions. 206661 known splicing junctions. 179921 novel splicing junctions.
sampling 75% (210564606) splicing reads. 388447 splicing junctions. 206873 known splicing junctions. 181574 novel splicing junctions.
sampling 80% (224602246) splicing reads. 389874 splicing junctions. 207035 known splicing junctions. 182839 novel splicing junctions.
sampling 85% (238639886) splicing reads. 391128 splicing junctions. 207195 known splicing junctions. 183933 novel splicing junctions.
sampling 90% (252677527) splicing reads. 392092 splicing junctions. 207299 known splicing junctions. 184793 novel splicing junctions.
sampling 95% (266715167) splicing reads. 392867 splicing junctions. 207392 known splicing junctions. 185475 novel splicing junctions.
sampling 100% (280752808) splicing reads. 393420 splicing junctions. 207461 known splicing junctions. 185959 novel splicing junctions.