[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2a8dd7-R1_A23WJ53LT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	293978039
Known Splicing Events:	291122121
Partial Novel Splicing Events:	1771558
Novel Splicing Events:	987866
Filtered Splicing Events:	96494

Total splicing  Junctions:	516532
Known Splicing Junctions:	251477
Partial Novel Splicing Junctions:	143690
Novel Splicing Junctions:	121365

===================================================================
Create BED file ...
Create Interact file ...