File Info

Filename
.command.log
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2c/9bd33d55ed898f45965117ca067d0e/.command.log
Size
6.5 KB
Task
NFCORE_RNAFUSION:RNAFUSION:STARFUSION_WORKFLOW:STARFUSION_FILTER (FFPE_HD789_01_RNA_13_A23WJ53LT4_1)
Attempt

Content

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  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d5/16e386de4b6722bebb7178ba756fe1/FFPE_HD789_01_RNA_13_A23WJ53LT4_1.starfusion.starfusion.fusion_predictions.tsv
  Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2c/9bd33d55ed898f45965117ca067d0e/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2c/9bd33d55ed898f45965117ca067d0e/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/stage-5ae5f7b1-ce8a-4a12-9374-820c751df9c5/e2/67df0cc053219f9b526f729943f15d/starfusion_filter_config.yaml
  Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/blacklist_breakpoint_database_2026-04-27.tsv
==> STAGING COMPLETE (6 inputs)

=== DEBUGGING INFORMATION ===
Current working directory: /tmp/nxf.Bb4vITNAgn
Input files:
  - starfusion_tsv: FFPE_HD789_01_RNA_13_A23WJ53LT4_1.starfusion.starfusion.fusion_predictions.tsv
  - blacklist: blacklist_hg38_GRCh38_v2.4.0.tsv.gz
  - filter_config: starfusion_filter_config.yaml
  - aih_blacklist_breakpoints: blacklist_breakpoint_database_2026-04-27.tsv

=== PYTHON INFORMATION ===
/usr/local/bin/python
Python 3.11.14
/usr/local/bin/python3
Python 3.11.14

=== SCRIPT LOCATION ===
Checking for filter_starfusion_main.py:
/tmp/nxf.Bb4vITNAgn/nextflow-bin/filter_starfusion_main.py
filter_starfusion_main.py not in current directory
bin/filter_starfusion_main.py not found
bin/ directory does not exist

=== ENVIRONMENT PATH ===
/tmp/nxf.Bb4vITNAgn/nextflow-bin:/usr/local/bin:/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin

=== CURRENT DIRECTORY CONTENTS ===
total 65524
drwx------. 3 1000 root     4096 Jun  8 10:29 .
drwxrwxrwx. 4 root root       86 Jun  8 10:29 ..
-rw-r--r--. 1 1000 root        0 Jun  8 10:29 .command.err
-rw-r--r--. 1 1000 root      884 Jun  8 10:29 .command.out
-rw-r--r--. 1 1000 root    14589 Jun  8 10:29 .command.run
-rw-r--r--. 1 1000 root     3236 Jun  8 10:29 .command.sh
-rw-r--r--. 1 1000 root        0 Jun  8 10:29 .command.trace
-rw-r--r--. 1 1000 root  1557792 Jun  8 10:29 FFPE_HD789_01_RNA_13_A23WJ53LT4_1.starfusion.starfusion.fusion_predictions.tsv
-rw-r--r--. 1 1000 root 10714998 Jun  8 10:29 blacklist_breakpoint_database_2026-04-27.tsv
-rw-r--r--. 1 1000 root 54779787 Jun  8 10:29 blacklist_hg38_GRCh38_v2.4.0.tsv.gz
drwxr-xr-x. 3 1000 root     4096 Jun  8 10:29 nextflow-bin
-rw-r--r--. 1 1000 root     4128 Jun  8 10:29 starfusion_filter_config.yaml

=== PROJECT DIRECTORY INFO ===
NXF_PROJECT_DIR: not set
baseDir: not set

=== Running filter script ===

================================================================================
STEP 1: LOADING STAR-FUSION PREDICTIONS
================================================================================
Loading STAR-Fusion predictions from: FFPE_HD789_01_RNA_13_A23WJ53LT4_1.starfusion.starfusion.fusion_predictions.tsv
  ✓ Loaded: 3783 fusions

================================================================================
STEP 2: CONVERTING TO BREAKPOINT_ID FORMAT
================================================================================
✓ Converted to breakpoint_ID format: 3783 valid, 0 invalid

================================================================================
STEP 3: FLAGGING WITH BLACKLIST
================================================================================
Loading blacklist (this may take a while)...
Loading blacklist from: blacklist_hg38_GRCh38_v2.4.0.tsv.gz
  Loaded 9381 single rules and 16721785 pair rules
  Indexed blacklist (bin_size=100000)
Flagging fusions with blacklist...
✓ Flagged 289 of 3783 fusions as blacklisted

================================================================================
STEP 3b: AIH CURATED BREAKPOINT BLACKLIST
================================================================================
Loading AIH blacklist table from: blacklist_breakpoint_database_2026-04-27.tsv
  Qualified breakpoint keys (orientations): 1378
  AIH curated blacklist: matched 145 fusion rows (breakpoint_ID in qualified set)

================================================================================
STEP 4: APPLYING YAML-BASED FILTER
================================================================================
✓ Filter initialized from: starfusion_filter_config.yaml
Applying filter criteria (adding CALL column)...
✓ Filter applied: 40 pass (CALL=1), 3743 fail (CALL=0)
  Total fusions: 3783 (all rows retained)
  Added FILTER column: PASS=40, FAIL=3743

✓ Verified: All 347 blacklisted fusions have CALL=0

================================================================================
STEP 5: GENERATING SUMMARY STATISTICS
================================================================================

================================================================================
FILTERING SUMMARY
================================================================================

Total fusions: 3783
  Pass (CALL=1): 40 (1.1%)
  Fail (CALL=0): 3743 (98.9%)

Blacklist Flagging:
  Total blacklisted: 347
  ✓ All 347 blacklisted fusions have CALL=0

Failure Reasons (top 10):
  Read count too low: 1 < 8: 2698
  Excluded SpliceType: INCL_NON_REF_SPLICE: 566
  Blacklisted: 202
  Blacklisted; aih_curated_blacklist score(3): 87
  aih_curated_blacklist score(3): 55
  Read count too low: 2 < 8: 50
  Excluded annotation pattern: HGNC_GENEFAM: 33
  Read count too low: 3 < 8: 13
  Excluded annotation pattern: GTEx_recurrent_StarF2019: 9
  Read count too low: 4 < 8: 8

Breakdown by SpliceType:
  INCL_NON_REF_SPLICE: 6 pass, 1089 fail (total: 1095)
  ONLY_REF_SPLICE: 34 pass, 2654 fail (total: 2688)

Read Count Statistics:
  Fusions with < 3 total reads: 3583
  Of these, 3583 have CALL=0

================================================================================
STEP 6: EXPORTING RESULTS
================================================================================
✓ Results exported to: FFPE_HD789_01_RNA_13_A23WJ53LT4_1.starfusion.starfusion.fusion_predictions.filtered.tsv
  Total fusions: 3783
  CALL=1 (pass): 40
  CALL=0 (fail): 3743
✓ Passing fusions (CALL=1) exported to: FFPE_HD789_01_RNA_13_A23WJ53LT4_1.starfusion.starfusion.fusion_predictions.filtered_pass_only.tsv
✓ Summary exported to: FFPE_HD789_01_RNA_13_A23WJ53LT4_1.starfusion.starfusion.fusion_predictions.filter_summary.txt

================================================================================
✓ FILTERING COMPLETE
================================================================================