+ mkdir aih-tih-sc-835699-R1_A23WJ53LT4_1 + all_qc -bam aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-835699-R1_A23WJ53LT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai 144522it [00:00, 1444994.00it/s] 307907it [00:00, 1556022.75it/s] 463510it [00:00, 1540461.17it/s] 625089it [00:00, 1569935.29it/s] 783124it [00:00, 1573646.97it/s] 940509it [00:00, 1544475.35it/s] 1104799it [00:00, 1576148.23it/s] 1273425it [00:00, 1610818.30it/s] 1443721it [00:00, 1639390.21it/s] 1610649it [00:01, 1648538.45it/s] 1779041it [00:01, 1659212.32it/s] 1945614it [00:01, 1661160.85it/s] 2117895it [00:01, 1679683.88it/s] 2287142it [00:01, 1683515.11it/s] 2462433it [00:01, 1704388.64it/s] 2635987it [00:01, 1713729.29it/s] 2807372it [00:01, 1707191.55it/s] 2978438it [00:01, 1708205.53it/s] 3151741it [00:01, 1715619.20it/s] 3323312it [00:02, 1690453.16it/s] 3493390it [00:02, 1693495.45it/s] 3662851it [00:02, 1693700.32it/s] 3832266it [00:02, 1687647.07it/s] 4001537it [00:02, 1689094.39it/s] 4170471it [00:02, 1688393.98it/s] 4340720it [00:02, 1692585.76it/s] 4513388it [00:02, 1702766.63it/s] 4683677it [00:02, 1702629.00it/s] 4853949it [00:02, 1700618.26it/s] 5024017it [00:03, 1690777.61it/s] 5196914it [00:03, 1702152.41it/s] 5369495it [00:03, 1709200.74it/s] 5541077it [00:03, 1711163.52it/s] 5719953it [00:03, 1734379.25it/s] 5893403it [00:03, 1734269.74it/s] 6066839it [00:03, 1719792.50it/s] 6238851it [00:03, 1707741.21it/s] 6409660it [00:03, 1694818.00it/s] 6579177it [00:03, 1691175.03it/s] 6748317it [00:04, 1686272.60it/s] 6919431it [00:04, 1693648.89it/s] 7090475it [00:04, 1698576.17it/s] 7262150it [00:04, 1703990.93it/s] 7435445it [00:04, 1712637.05it/s] 7607983it [00:04, 1716436.02it/s] 7779636it [00:04, 1715602.14it/s] 7951203it [00:04, 1702402.53it/s] 8121470it [00:04, 1701191.55it/s] 8291607it [00:04, 1700946.30it/s] 8461714it [00:05, 1681064.53it/s] 8629876it [00:05, 1644582.53it/s] 8802302it [00:05, 1667927.11it/s] 8972268it [00:05, 1677272.80it/s] 9144266it [00:05, 1689928.16it/s] 9317787it [00:05, 1703391.60it/s] 9491662it [00:05, 1713925.59it/s] 9663124it [00:05, 1705777.38it/s] 9833757it [00:05, 1705852.90it/s] 10004381it [00:05, 1701414.46it/s] 10177579it [00:06, 1710523.63it/s] 10349726it [00:06, 1713763.69it/s] 10521121it [00:06, 1697827.95it/s] 10691915it [00:06, 1700822.54it/s] 10863194it [00:06, 1704348.82it/s] 11033797it [00:06, 1704837.03it/s] 11204299it [00:06, 1704675.84it/s] 11374779it [00:06, 1701027.43it/s] 11546601it [00:06, 1706083.93it/s] 11717218it [00:06, 1706071.22it/s] 11888427it [00:07, 1707849.01it/s] 12059217it [00:07, 1705519.00it/s] 12231092it [00:07, 1709468.59it/s] 12402042it [00:07, 1706057.31it/s] 12468521it [00:07, 1687521.02it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-835699-R1_A23WJ53LT4_1.md.bai + merge_qc -folders aih-tih-sc-835699-R1_A23WJ53LT4_1/aih-tih-sc-835699-R1_A23WJ53LT4_1.md -output aih-tih-sc-835699-R1_A23WJ53LT4_1/aih-tih-sc-835699-R1_A23WJ53LT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-835699-R1_A23WJ53LT4_1/aih-tih-sc-835699-R1_A23WJ53LT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-835699-R1_A23WJ53LT4_1/aih-tih-sc-835699-R1_A23WJ53LT4_1_full_basic_stats.tsv