Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/57/a9b77c2df81bd4d81ce773031ed290/.command.sh Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/57/a9b77c2df81bd4d81ce773031ed290/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/88/86a32cbbd55921edc09b43b2516f09/aih-tih-sc-488877-R1_A23WJ53LT4_1_1.fastp.fastq.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/88/86a32cbbd55921edc09b43b2516f09/aih-tih-sc-488877-R1_A23WJ53LT4_1_2.fastp.fastq.gz ==> STAGING COMPLETE (6 inputs) STAR --genomeDir ref_genome.fa.star.idx --readFilesIn input1/aih-tih-sc-488877-R1_A23WJ53LT4_1_1.fastp.fastq.gz input2/aih-tih-sc-488877-R1_A23WJ53LT4_1_2.fastp.fastq.gz --runThreadN 24 --outFileNamePrefix aih-tih-sc-488877-R1_A23WJ53LT4_1. --sjdbGTFfile ref_annot.gtf --outSAMattrRGline ID:aih-tih-sc-488877-R1_A23WJ53LT4_1 SM:aih-tih-sc-488877-R1_A23WJ53LT4_1 --twopassMode None --chimSegmentMin 12 --chimJunctionOverhangMin 5 --chimMultimapNmax 50 --chimScoreJunctionNonGTAG -4 --chimScoreSeparation 1 --alignIntronMax 140000 --outReadsUnmapped None --readFilesCommand zcat --outSAMtype BAM SortedByCoordinate --outSAMstrandField intronMotif --outSAMunmapped Within --chimOutJunctionFormat 1 --alignSJDBoverhangMin 10 --alignMatesGapMax 100000 --alignSJstitchMismatchNmax 5 -1 5 5 --chimMultimapScoreRange 3 --chimNonchimScoreDropMin 10 --peOverlapNbasesMin 12 --peOverlapMMp 0.1 --alignInsertionFlush Right --alignSplicedMateMapLmin 30 --alignSplicedMateMapLminOverLmate 0.5 --quantMode GeneCounts --runRNGseed 1 --chimOutType Junctions WithinBAM --limitBAMsortRAM 38654705664 STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source Jun 08 07:54:24 ..... started STAR run Jun 08 07:54:24 ..... loading genome Jun 08 07:54:57 ..... processing annotations GTF Jun 08 07:55:12 ..... inserting junctions into the genome indices Jun 08 07:57:02 ..... started mapping Jun 08 08:37:54 ..... finished mapping Jun 08 08:37:59 ..... started sorting BAM Jun 08 08:42:23 ..... finished successfully