+ mkdir aih-tih-sc-560d56-R1_A23WJ53LT4_1 + all_qc -bam aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-560d56-R1_A23WJ53LT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai 114406it [00:00, 1108726.59it/s] 231503it [00:00, 1117052.86it/s] 343501it [00:00, 1118188.54it/s] 455321it [00:00, 1069025.39it/s] 592998it [00:00, 1175904.88it/s] 743635it [00:00, 1285473.94it/s] 909663it [00:00, 1405929.16it/s] 1073176it [00:00, 1478017.02it/s] 1239033it [00:00, 1533970.74it/s] 1406823it [00:01, 1578117.59it/s] 1578343it [00:01, 1619862.20it/s] 1740554it [00:01, 1592020.03it/s] 1900014it [00:01, 1574637.44it/s] 2059966it [00:01, 1581867.03it/s] 2218306it [00:01, 1457281.52it/s] 2390431it [00:01, 1531524.36it/s] 2556457it [00:01, 1568396.46it/s] 2725206it [00:01, 1602934.80it/s] 2886761it [00:01, 1606404.25it/s] 3049643it [00:02, 1612896.24it/s] 3221011it [00:02, 1642763.57it/s] 3387117it [00:02, 1648176.02it/s] 3552250it [00:02, 1645399.13it/s] 3722887it [00:02, 1663539.25it/s] 3893453it [00:02, 1676060.59it/s] 4061179it [00:02, 1620465.19it/s] 4223698it [00:02, 1617189.24it/s] 4385742it [00:02, 1610518.35it/s] 4547015it [00:02, 1601675.11it/s] 4711132it [00:03, 1613272.99it/s] 4872585it [00:03, 1490516.57it/s] 5023485it [00:03, 1482509.58it/s] 5178710it [00:03, 1502335.72it/s] 5333693it [00:03, 1516029.92it/s] 5498666it [00:03, 1555073.88it/s] 5654795it [00:03, 1508075.21it/s] 5806286it [00:03, 1461361.86it/s] 5953666it [00:03, 1464851.50it/s] 6100634it [00:04, 1441245.40it/s] 6265238it [00:04, 1500431.17it/s] 6415746it [00:04, 1483460.43it/s] 6577676it [00:04, 1523106.94it/s] 6742348it [00:04, 1559471.28it/s] 6898601it [00:04, 1527525.07it/s] 7051680it [00:04, 1526558.91it/s] 7216863it [00:04, 1563444.58it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai 7388240it [00:04, 1607944.07it/s] 7559820it [00:04, 1639976.11it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai 7727065it [00:05, 1649565.83it/s] 7892152it [00:05, 1631681.70it/s] 8055449it [00:05, 1614101.00it/s] 8216976it [00:05, 1594429.82it/s] 8378646it [00:05, 1600901.86it/s] 8550470it [00:05, 1635587.77it/s] 8720106it [00:05, 1653610.02it/s] 8894355it [00:05, 1679971.76it/s] 9067198it [00:05, 1694375.84it/s] 9240491it [00:05, 1705874.08it/s] 9411129it [00:06, 1704322.21it/s] 9525416it [00:06, 1555467.25it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-560d56-R1_A23WJ53LT4_1.md.bai + merge_qc -folders aih-tih-sc-560d56-R1_A23WJ53LT4_1/aih-tih-sc-560d56-R1_A23WJ53LT4_1.md -output aih-tih-sc-560d56-R1_A23WJ53LT4_1/aih-tih-sc-560d56-R1_A23WJ53LT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-560d56-R1_A23WJ53LT4_1/aih-tih-sc-560d56-R1_A23WJ53LT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-560d56-R1_A23WJ53LT4_1/aih-tih-sc-560d56-R1_A23WJ53LT4_1_full_basic_stats.tsv