+ mkdir aih-tih-sc-af602e-R1_A23WJ53LT4_1 + all_qc -bam aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-af602e-R1_A23WJ53LT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai 0it [00:00, ?it/s] 134719it [00:00, 1346908.69it/s] 292027it [00:00, 1479906.82it/s] 453806it [00:00, 1539306.65it/s] 615795it [00:00, 1571055.89it/s] 782946it [00:00, 1607230.90it/s] 952734it [00:00, 1638018.60it/s] 1118409it [00:00, 1644126.18it/s] 1285991it [00:00, 1654086.77it/s] 1455975it [00:00, 1668371.88it/s] 1626812it [00:01, 1680704.77it/s] 1797319it [00:01, 1688020.91it/s] 1966122it [00:01, 1685712.50it/s] 2134695it [00:01, 1679675.91it/s] 2305998it [00:01, 1689706.15it/s] 2476616it [00:01, 1694543.23it/s] 2647834it [00:01, 1699829.08it/s] 2817822it [00:01, 1690985.83it/s] 2992346it [00:01, 1707188.48it/s] 3167169it [00:01, 1719463.36it/s] 3339131it [00:02, 1694892.39it/s] 3508906it [00:02, 1695725.10it/s] 3678539it [00:02, 1694025.31it/s] 3849936it [00:02, 1699864.45it/s] 4023076it [00:02, 1709221.85it/s] 4194026it [00:02, 1689456.80it/s] 4363313it [00:02, 1690453.91it/s] 4534106it [00:02, 1695506.27it/s] 4705880it [00:02, 1701968.09it/s] 4881167it [00:02, 1717143.72it/s] 5052907it [00:03, 1707600.78it/s] 5223695it [00:03, 1687241.05it/s] 5398346it [00:03, 1704777.32it/s] 5572367it [00:03, 1715288.01it/s] 5745057it [00:03, 1718667.62it/s] 5918453it [00:03, 1723078.85it/s] 6092289it [00:03, 1727628.51it/s] 6271666it [00:03, 1747329.59it/s] 6446420it [00:03, 1717925.92it/s] 6618604it [00:03, 1718976.20it/s] 6790588it [00:04, 1713299.48it/s] 6968719it [00:04, 1733505.74it/s] 7142690it [00:04, 1735222.75it/s] 7316256it [00:04, 1726477.33it/s] 7488941it [00:04, 1711670.73it/s] 7660623it [00:04, 1713181.25it/s] 7831975it [00:04, 1702633.28it/s] 8002270it [00:04, 1695691.42it/s] 8173971it [00:04, 1702010.09it/s] 8344194it [00:04, 1699699.79it/s] 8514179it [00:05, 1698371.80it/s] 8684026it [00:05, 1698165.84it/s] 8855427it [00:05, 1702886.85it/s] 9025722it [00:05, 1687072.77it/s] 9195506it [00:05, 1690260.31it/s] 9366336it [00:05, 1695524.75it/s] 9535911it [00:05, 1695451.23it/s] 9706323it [00:05, 1698027.83it/s] 9877058it [00:05, 1700805.90it/s] 10048854it [00:05, 1705922.08it/s] 10219453it [00:06, 1703684.77it/s] 10389827it [00:06, 1697345.81it/s] 10560243it [00:06, 1699368.34it/s] 10732261it [00:06, 1705455.93it/s] 10904039it [00:06, 1709007.50it/s] 11075218it [00:06, 1709673.06it/s] 11246189it [00:06, 1696735.68it/s] 11416132it [00:06, 1697522.68it/s] 11585902it [00:06, 1693851.99it/s] 11756905it [00:06, 1698657.39it/s] 11926782it [00:07, 1697332.52it/s] 12097401it [00:07, 1699964.67it/s] 12270004it [00:07, 1707753.62it/s] 12443710it [00:07, 1716519.21it/s] 12615368it [00:07, 1711194.48it/s] 12789940it [00:07, 1721505.83it/s] 12962099it [00:07, 1718038.82it/s] 13133910it [00:07, 1710907.34it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai 13305011it [00:07, 1701753.71it/s] 13475200it [00:07, 1670439.86it/s] 13642363it [00:08, 1656172.97it/s] 13809582it [00:08, 1660818.54it/s] 13979952it [00:08, 1673496.78it/s] 14151380it [00:08, 1685602.90it/s] 14319998it [00:08, 1676567.53it/s] 14487701it [00:08, 1673603.96it/s] 14655093it [00:08, 1663157.85it/s] 14824831it [00:08, 1673316.13it/s] 14992193it [00:08, 1673384.57it/s] 15159553it [00:08, 1672490.88it/s] 15326827it [00:09, 1672435.91it/s] 15494081it [00:09, 1664302.63it/s] 15544345it [00:09, 1690684.79it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-af602e-R1_A23WJ53LT4_1.md.bai + merge_qc -folders aih-tih-sc-af602e-R1_A23WJ53LT4_1/aih-tih-sc-af602e-R1_A23WJ53LT4_1.md -output aih-tih-sc-af602e-R1_A23WJ53LT4_1/aih-tih-sc-af602e-R1_A23WJ53LT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-af602e-R1_A23WJ53LT4_1/aih-tih-sc-af602e-R1_A23WJ53LT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-af602e-R1_A23WJ53LT4_1/aih-tih-sc-af602e-R1_A23WJ53LT4_1_full_basic_stats.tsv