+ mkdir aih-tih-sc-2798bb-R1_A23WJ53LT4_1 + all_qc -bam aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-2798bb-R1_A23WJ53LT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai 136493it [00:00, 1364846.70it/s] 288985it [00:00, 1458819.74it/s] 451807it [00:00, 1536130.61it/s] 610972it [00:00, 1557955.86it/s] 774725it [00:00, 1586621.89it/s] 939453it [00:00, 1607199.32it/s] 1104235it [00:00, 1620305.86it/s] 1271598it [00:00, 1637260.40it/s] 1436232it [00:00, 1640070.95it/s] 1601965it [00:01, 1645370.22it/s] 1766727it [00:01, 1646014.77it/s] 1931878it [00:01, 1647666.54it/s] 2100094it [00:01, 1658095.40it/s] 2267572it [00:01, 1663110.07it/s] 2434611it [00:01, 1665280.92it/s] 2603512it [00:01, 1672394.78it/s] 2770752it [00:01, 1668996.39it/s] 2937654it [00:01, 1657061.88it/s] 3103380it [00:01, 1656557.90it/s] 3269049it [00:02, 1642379.69it/s] 3435813it [00:02, 1649853.41it/s] 3603410it [00:02, 1657619.13it/s] 3769639it [00:02, 1658992.47it/s] 3936403it [00:02, 1661444.39it/s] 4102900it [00:02, 1662475.36it/s] 4269887it [00:02, 1664665.90it/s] 4436601it [00:02, 1665384.01it/s] 4605378it [00:02, 1672077.26it/s] 4772590it [00:02, 1670378.21it/s] 4939631it [00:03, 1654535.96it/s] 5105351it [00:03, 1655288.58it/s] 5274462it [00:03, 1665944.94it/s] 5444545it [00:03, 1676219.54it/s] 5616346it [00:03, 1688699.95it/s] 5785658it [00:03, 1689996.23it/s] 5956835it [00:03, 1696456.04it/s] 6127383it [00:03, 1698912.28it/s] 6297281it [00:03, 1694925.83it/s] 6466781it [00:03, 1677162.64it/s] 6634541it [00:04, 1628870.91it/s] 6800575it [00:04, 1638043.16it/s] 6969463it [00:04, 1652978.65it/s] 7137852it [00:04, 1661974.03it/s] 7305614it [00:04, 1666546.48it/s] 7473630it [00:04, 1670577.97it/s] 7642684it [00:04, 1676510.65it/s] 7810392it [00:04, 1658825.07it/s] 7976352it [00:04, 1625552.74it/s] 8145808it [00:04, 1645728.18it/s] 8310547it [00:05, 1614038.26it/s] 8472162it [00:05, 1578275.08it/s] 8630239it [00:05, 1559080.12it/s] 8786316it [00:05, 1532406.65it/s] 8951924it [00:05, 1568207.09it/s] 9117310it [00:05, 1593256.07it/s] 9283203it [00:05, 1612598.90it/s] 9451086it [00:05, 1632207.27it/s] 9619074it [00:05, 1646367.30it/s] 9786269it [00:05, 1653978.78it/s] 9951757it [00:06, 1651754.43it/s] 10118883it [00:06, 1657565.37it/s] 10284686it [00:06, 1644137.15it/s] 10453338it [00:06, 1656690.91it/s] 10624953it [00:06, 1674354.69it/s] 10792432it [00:06, 1672449.95it/s] 10959707it [00:06, 1662188.42it/s] 11127132it [00:06, 1665763.77it/s] 11294536it [00:06, 1668212.66it/s] 11463143it [00:06, 1673534.40it/s] 11630512it [00:07, 1654978.23it/s] 11796305it [00:07, 1655835.92it/s] 11961927it [00:07, 1650349.45it/s] 12130468it [00:07, 1660777.67it/s] 12297487it [00:07, 1663568.97it/s] 12463964it [00:07, 1663909.52it/s] 12630711it [00:07, 1664958.91it/s] 12797217it [00:07, 1659890.88it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai 12963216it [00:07, 1657792.60it/s] 13129003it [00:07, 1619472.48it/s] 13291141it [00:08, 1545009.07it/s] 13446393it [00:08, 1521466.67it/s] 13599051it [00:08, 1509271.24it/s] 13764135it [00:08, 1550133.31it/s] 13926626it [00:08, 1571956.54it/s] 14084153it [00:08, 1506403.84it/s] 14237023it [00:08, 1512791.76it/s] 14402932it [00:08, 1555330.53it/s] 14566702it [00:08, 1579467.56it/s] 14730284it [00:09, 1596099.42it/s] 14892712it [00:09, 1604445.68it/s] 15053787it [00:09, 1606190.98it/s] 15135055it [00:09, 1632687.86it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md.bai + merge_qc -folders aih-tih-sc-2798bb-R1_A23WJ53LT4_1/aih-tih-sc-2798bb-R1_A23WJ53LT4_1.md -output aih-tih-sc-2798bb-R1_A23WJ53LT4_1/aih-tih-sc-2798bb-R1_A23WJ53LT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-2798bb-R1_A23WJ53LT4_1/aih-tih-sc-2798bb-R1_A23WJ53LT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-2798bb-R1_A23WJ53LT4_1/aih-tih-sc-2798bb-R1_A23WJ53LT4_1_full_basic_stats.tsv