[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1dd7a6-R1_A23WJ53LT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	333480689
Known Splicing Events:	330566326
Partial Novel Splicing Events:	1546150
Novel Splicing Events:	1238795
Filtered Splicing Events:	129418

Total splicing  Junctions:	468205
Known Splicing Junctions:	253990
Partial Novel Splicing Junctions:	114455
Novel Splicing Junctions:	99760

===================================================================
Create BED file ...
Create Interact file ...