Done. -parsing GTF file: /tmp/nxf.iF3FKqFePk/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.iF3FKqFePk/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.iF3FKqFePk/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1_1.fastp.fastq.gz /tmp/nxf.iF3FKqFePk/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.iF3FKqFePk/fi_workdir/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.iF3FKqFePk/fi_workdir/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.star.sortedByCoord.out.bam * Running CMD: samtools index aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.star.sortedByCoord.out.bam 11:23:51 : INFO : Done. -parsing GTF file: /tmp/nxf.iF3FKqFePk/fi_workdir/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.gtf -parsing /tmp/nxf.iF3FKqFePk/fi_workdir/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] [90000] [100000] [110000] [120000] -done parsing /tmp/nxf.iF3FKqFePk/fi_workdir/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 35, 'small anchor length' => 12, 'seq-similar region overlap' => 23, ' ** passed ** ' => 1427, 'per_id < 96' => 445, 'excessive soft clipping' => 989, 'num genes matched < 2' => 10 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.iF3FKqFePk/fi_workdir/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] -fusion SPANNING read extraction for scaff: AC092807.3--DDAH1 -fusion SPANNING read extraction for scaff: LINC00381--NKAIN2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] -fusion SPANNING read extraction for scaff: PCDHA10--PCDHAC1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] -fusion SPANNING read extraction for scaff: PCDHA10--PCDHA13 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] -fusion SPANNING read extraction for scaff: PCDHA10--PCDHAC2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] -fusion SPANNING read extraction for scaff: PMS2P6--CCDC146 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] -fusion SPANNING read extraction for scaff: STON1--GTF2A1L [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] -fusion SPANNING read extraction for scaff: TLK2--AC240565.1 [1000] [2000] [3000] [4000] -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 -filtered reads reasons: $VAR1 = { 'seq similar region alignment' => 5744, 'lacks exon overlap' => 762 }; EM: Starting log likelihood: -1968.251691 EM: Round [1] log likelihood: -1967.880041 EM: Round [2] log likelihood: -1967.793002 EM: Round [3] log likelihood: -1967.714731 EM: Round [4] log likelihood: -1967.643966 EM: Round [5] log likelihood: -1967.579838 EM: Round [6] log likelihood: -1967.521601 EM: Round [7] log likelihood: -1967.468617 EM: Round [8] log likelihood: -1967.420332 EM: Round [9] log likelihood: -1967.376263 EM: Round [10] log likelihood: -1967.335988 EM: Round [11] log likelihood: -1967.299138 EM: Round [12] log likelihood: -1967.265383 EM: Round [13] log likelihood: -1967.234433 EM: Round [14] log likelihood: -1967.206031 EM: Round [15] log likelihood: -1967.179945 EM: Round [16] log likelihood: -1967.155968 EM: Round [17] log likelihood: -1967.133916 EM: Round [18] log likelihood: -1967.113621 EM: Round [19] log likelihood: -1967.094932 EM: Round [20] log likelihood: -1967.077715 EM: Round [21] log likelihood: -1967.061845 EM: Round [22] log likelihood: -1967.047211 EM: Round [23] log likelihood: -1967.033711 EM: Round [24] log likelihood: -1967.021253 EM: Round [25] log likelihood: -1967.009752 EM: Round [26] log likelihood: -1966.999132 EM: Round [27] log likelihood: -1966.989323 EM: Round [28] log likelihood: -1966.980260 EM: Round [29] log likelihood: -1966.971885 EM: Round [30] log likelihood: -1966.964143 EM: Round [31] log likelihood: -1966.956985 EM: Round [32] log likelihood: -1966.950367 EM: Round [33] log likelihood: -1966.944246 EM: Round [34] log likelihood: -1966.938583 EM: Round [35] log likelihood: -1966.933345 EM: Round [36] log likelihood: -1966.928498 EM: Round [37] log likelihood: -1966.924013 EM: Round [38] log likelihood: -1966.919861 EM: Round [39] log likelihood: -1966.916019 EM: Round [40] log likelihood: -1966.912462 EM: Round [41] log likelihood: -1966.909170 EM: Round [42] log likelihood: -1966.906121 EM: Round [43] log likelihood: -1966.903298 EM: Round [44] log likelihood: -1966.900684 EM: Round [45] log likelihood: -1966.898264 EM: Round [46] log likelihood: -1966.896022 EM: Round [47] log likelihood: -1966.893946 EM: Round [48] log likelihood: -1966.892023 EM: Round [49] log likelihood: -1966.890242 EM: Round [50] log likelihood: -1966.888592 EM: Round [51] log likelihood: -1966.887064 EM: Round [52] log likelihood: -1966.885648 EM: Round [53] log likelihood: -1966.884336 EM: Round [54] log likelihood: -1966.883121 EM: Round [55] log likelihood: -1966.881995 EM: Round [56] log likelihood: -1966.880952 EM: Round [57] log likelihood: -1966.879986 EM: Round [58] log likelihood: -1966.879090 EM: Round [59] log likelihood: -1966.878261 EM: Round [60] log likelihood: -1966.877492 EM: Round [61] log likelihood: -1966.876780 EM: Round [62] log likelihood: -1966.876119 EM: Round [63] log likelihood: -1966.875508 EM: Round [64] log likelihood: -1966.874941 EM: Round [65] log likelihood: -1966.874416 EM: Round [66] log likelihood: -1966.873929 EM: Round [67] log likelihood: -1966.873478 EM: Round [68] log likelihood: -1966.873060 EM: Round [69] log likelihood: -1966.872673 EM: Round [70] log likelihood: -1966.872314 EM: Round [71] log likelihood: -1966.871981 EM: Round [72] log likelihood: -1966.871673 EM: Round [73] log likelihood: -1966.871387 EM: Round [74] log likelihood: -1966.871123 EM: Round [75] log likelihood: -1966.870877 EM: Round [76] log likelihood: -1966.870650 EM: Round [77] log likelihood: -1966.870439 EM: Round [78] log likelihood: -1966.870244 EM: Round [79] log likelihood: -1966.870063 EM: Round [80] log likelihood: -1966.869895 EM: Round [81] log likelihood: -1966.869740 EM: Round [82] log likelihood: -1966.869596 EM: Round [83] log likelihood: -1966.869462 EM: Round [84] log likelihood: -1966.869338 EM: Round [85] log likelihood: -1966.869224 EM: Round [86] log likelihood: -1966.869117 EM: Round [87] log likelihood: -1966.869019 EM: Stopping iterations at round 87 due to insufficient improvement in likelihood. [bam_sort_core] merging from 0 files and 8 in-memory blocks... -extracting read coordinates from /tmp/nxf.iF3FKqFePk/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.consolidated.bam into /tmp/nxf.iF3FKqFePk/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.consolidated.bam.read_coords CMD: touch /tmp/nxf.iF3FKqFePk/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.iF3FKqFePk/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.consolidated.bam.read_coords > /tmp/nxf.iF3FKqFePk/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.iF3FKqFePk/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.consolidated.bam.frag_coords > /tmp/nxf.iF3FKqFePk/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.iF3FKqFePk/fi_workdir/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.iF3FKqFePk/fi_workdir/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.iF3FKqFePk/fi_workdir/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.iF3FKqFePk/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.post_blast_and_promiscuity_filter -done, see /tmp/nxf.iF3FKqFePk/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -1901.575315 EM: Round [1] log likelihood: -1901.501593 EM: Round [2] log likelihood: -1901.501430 EM: Round [3] log likelihood: -1901.501429 EM: Stopping iterations at round 3 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.iF3FKqFePk/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1_1.fastp.fastq.gz: 295863290 Warning - not locating file: /tmp/nxf.iF3FKqFePk/aih-tih-sc-38a0a3-R1_A23WJ53LT4_1.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000280136.2] WARNING, no entry stored in dbm for [ENSG00000174384.9] WARNING, no entry stored in dbm for [ENSG00000174384.9] WARNING, no entry stored in dbm for [ENSG00000174384.9] WARNING, no entry stored in dbm for [ENSG00000174384.9] WARNING, no entry stored in dbm for [ENSG00000174384.9] WARNING, no entry stored in dbm for [ENSG00000174384.9] WARNING, no entry stored in dbm for [ENSG00000226240.2] WARNING, no entry stored in dbm for [ENSG00000226240.2] WARNING, no entry stored in dbm for [ENSG00000226049.3] WARNING, no entry stored in dbm for [ENSG00000260404.3] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000282057.1] ls: cannot access 'IGV_inputs': No such file or directory