[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-284856-R1_A23WJ53LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (3881327) splicing reads. 191818 splicing junctions. 163045 known splicing junctions. 28773 novel splicing junctions.
sampling 10% (7762654) splicing reads. 226562 splicing junctions. 176917 known splicing junctions. 49645 novel splicing junctions.
sampling 15% (11643981) splicing reads. 251251 splicing junctions. 184660 known splicing junctions. 66591 novel splicing junctions.
sampling 20% (15525309) splicing reads. 271194 splicing junctions. 189967 known splicing junctions. 81227 novel splicing junctions.
sampling 25% (19406636) splicing reads. 287903 splicing junctions. 193937 known splicing junctions. 93966 novel splicing junctions.
sampling 30% (23287963) splicing reads. 302138 splicing junctions. 196990 known splicing junctions. 105148 novel splicing junctions.
sampling 35% (27169290) splicing reads. 314577 splicing junctions. 199444 known splicing junctions. 115133 novel splicing junctions.
sampling 40% (31050618) splicing reads. 325458 splicing junctions. 201447 known splicing junctions. 124011 novel splicing junctions.
sampling 45% (34931945) splicing reads. 335116 splicing junctions. 203131 known splicing junctions. 131985 novel splicing junctions.
sampling 50% (38813272) splicing reads. 343862 splicing junctions. 204548 known splicing junctions. 139314 novel splicing junctions.
sampling 55% (42694599) splicing reads. 351527 splicing junctions. 205734 known splicing junctions. 145793 novel splicing junctions.
sampling 60% (46575927) splicing reads. 358287 splicing junctions. 206751 known splicing junctions. 151536 novel splicing junctions.
sampling 65% (50457254) splicing reads. 364378 splicing junctions. 207667 known splicing junctions. 156711 novel splicing junctions.
sampling 70% (54338581) splicing reads. 369675 splicing junctions. 208425 known splicing junctions. 161250 novel splicing junctions.
sampling 75% (58219908) splicing reads. 374336 splicing junctions. 209096 known splicing junctions. 165240 novel splicing junctions.
sampling 80% (62101236) splicing reads. 378469 splicing junctions. 209654 known splicing junctions. 168815 novel splicing junctions.
sampling 85% (65982563) splicing reads. 382076 splicing junctions. 210168 known splicing junctions. 171908 novel splicing junctions.
sampling 90% (69863890) splicing reads. 385104 splicing junctions. 210618 known splicing junctions. 174486 novel splicing junctions.
sampling 95% (73745217) splicing reads. 387681 splicing junctions. 210953 known splicing junctions. 176728 novel splicing junctions.
sampling 100% (77626545) splicing reads. 389738 splicing junctions. 211207 known splicing junctions. 178531 novel splicing junctions.