[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2d4b78-R1_A23WJ53LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (16044639) splicing reads. 252152 splicing junctions. 182954 known splicing junctions. 69198 novel splicing junctions.
sampling 10% (32089278) splicing reads. 304406 splicing junctions. 195540 known splicing junctions. 108866 novel splicing junctions.
sampling 15% (48133918) splicing reads. 340875 splicing junctions. 202343 known splicing junctions. 138532 novel splicing junctions.
sampling 20% (64178557) splicing reads. 369310 splicing junctions. 206858 known splicing junctions. 162452 novel splicing junctions.
sampling 25% (80223197) splicing reads. 392194 splicing junctions. 209997 known splicing junctions. 182197 novel splicing junctions.
sampling 30% (96267836) splicing reads. 411553 splicing junctions. 212506 known splicing junctions. 199047 novel splicing junctions.
sampling 35% (112312475) splicing reads. 427841 splicing junctions. 214474 known splicing junctions. 213367 novel splicing junctions.
sampling 40% (128357115) splicing reads. 441825 splicing junctions. 216067 known splicing junctions. 225758 novel splicing junctions.
sampling 45% (144401754) splicing reads. 454108 splicing junctions. 217353 known splicing junctions. 236755 novel splicing junctions.
sampling 50% (160446394) splicing reads. 464936 splicing junctions. 218482 known splicing junctions. 246454 novel splicing junctions.
sampling 55% (176491033) splicing reads. 474459 splicing junctions. 219342 known splicing junctions. 255117 novel splicing junctions.
sampling 60% (192535672) splicing reads. 482767 splicing junctions. 220118 known splicing junctions. 262649 novel splicing junctions.
sampling 65% (208580312) splicing reads. 490047 splicing junctions. 220813 known splicing junctions. 269234 novel splicing junctions.
sampling 70% (224624951) splicing reads. 496277 splicing junctions. 221363 known splicing junctions. 274914 novel splicing junctions.
sampling 75% (240669591) splicing reads. 502032 splicing junctions. 221844 known splicing junctions. 280188 novel splicing junctions.
sampling 80% (256714230) splicing reads. 506881 splicing junctions. 222258 known splicing junctions. 284623 novel splicing junctions.
sampling 85% (272758869) splicing reads. 511081 splicing junctions. 222623 known splicing junctions. 288458 novel splicing junctions.
sampling 90% (288803509) splicing reads. 514656 splicing junctions. 222929 known splicing junctions. 291727 novel splicing junctions.
sampling 95% (304848148) splicing reads. 517639 splicing junctions. 223190 known splicing junctions. 294449 novel splicing junctions.
sampling 100% (320892788) splicing reads. 520163 splicing junctions. 223417 known splicing junctions. 296746 novel splicing junctions.