[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-09b6a3-R1_A23WJ53LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (9818583) splicing reads. 222029 splicing junctions. 171503 known splicing junctions. 50526 novel splicing junctions.
sampling 10% (19637167) splicing reads. 253519 splicing junctions. 180840 known splicing junctions. 72679 novel splicing junctions.
sampling 15% (29455750) splicing reads. 270889 splicing junctions. 185131 known splicing junctions. 85758 novel splicing junctions.
sampling 20% (39274334) splicing reads. 282274 splicing junctions. 187640 known splicing junctions. 94634 novel splicing junctions.
sampling 25% (49092918) splicing reads. 290252 splicing junctions. 189292 known splicing junctions. 100960 novel splicing junctions.
sampling 30% (58911501) splicing reads. 296063 splicing junctions. 190438 known splicing junctions. 105625 novel splicing junctions.
sampling 35% (68730085) splicing reads. 300619 splicing junctions. 191285 known splicing junctions. 109334 novel splicing junctions.
sampling 40% (78548668) splicing reads. 304254 splicing junctions. 191973 known splicing junctions. 112281 novel splicing junctions.
sampling 45% (88367252) splicing reads. 307197 splicing junctions. 192487 known splicing junctions. 114710 novel splicing junctions.
sampling 50% (98185836) splicing reads. 309555 splicing junctions. 192884 known splicing junctions. 116671 novel splicing junctions.
sampling 55% (108004419) splicing reads. 311540 splicing junctions. 193248 known splicing junctions. 118292 novel splicing junctions.
sampling 60% (117823003) splicing reads. 313175 splicing junctions. 193515 known splicing junctions. 119660 novel splicing junctions.
sampling 65% (127641586) splicing reads. 314623 splicing junctions. 193740 known splicing junctions. 120883 novel splicing junctions.
sampling 70% (137460170) splicing reads. 315820 splicing junctions. 193931 known splicing junctions. 121889 novel splicing junctions.
sampling 75% (147278754) splicing reads. 316810 splicing junctions. 194086 known splicing junctions. 122724 novel splicing junctions.
sampling 80% (157097337) splicing reads. 317626 splicing junctions. 194210 known splicing junctions. 123416 novel splicing junctions.
sampling 85% (166915921) splicing reads. 318255 splicing junctions. 194328 known splicing junctions. 123927 novel splicing junctions.
sampling 90% (176734504) splicing reads. 318732 splicing junctions. 194401 known splicing junctions. 124331 novel splicing junctions.
sampling 95% (186553088) splicing reads. 319071 splicing junctions. 194441 known splicing junctions. 124630 novel splicing junctions.
sampling 100% (196371672) splicing reads. 319276 splicing junctions. 194468 known splicing junctions. 124808 novel splicing junctions.