[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-33eb27-R1_A23WJ53LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (14096512) splicing reads. 247955 splicing junctions. 179771 known splicing junctions. 68184 novel splicing junctions.
sampling 10% (28193025) splicing reads. 297207 splicing junctions. 191155 known splicing junctions. 106052 novel splicing junctions.
sampling 15% (42289538) splicing reads. 329226 splicing junctions. 197067 known splicing junctions. 132159 novel splicing junctions.
sampling 20% (56386050) splicing reads. 353014 splicing junctions. 200801 known splicing junctions. 152213 novel splicing junctions.
sampling 25% (70482563) splicing reads. 370794 splicing junctions. 203314 known splicing junctions. 167480 novel splicing junctions.
sampling 30% (84579076) splicing reads. 385000 splicing junctions. 205164 known splicing junctions. 179836 novel splicing junctions.
sampling 35% (98675588) splicing reads. 396339 splicing junctions. 206608 known splicing junctions. 189731 novel splicing junctions.
sampling 40% (112772101) splicing reads. 405856 splicing junctions. 207714 known splicing junctions. 198142 novel splicing junctions.
sampling 45% (126868614) splicing reads. 413614 splicing junctions. 208558 known splicing junctions. 205056 novel splicing junctions.
sampling 50% (140965127) splicing reads. 420280 splicing junctions. 209342 known splicing junctions. 210938 novel splicing junctions.
sampling 55% (155061639) splicing reads. 425828 splicing junctions. 209938 known splicing junctions. 215890 novel splicing junctions.
sampling 60% (169158152) splicing reads. 430543 splicing junctions. 210454 known splicing junctions. 220089 novel splicing junctions.
sampling 65% (183254665) splicing reads. 434547 splicing junctions. 210886 known splicing junctions. 223661 novel splicing junctions.
sampling 70% (197351177) splicing reads. 437943 splicing junctions. 211255 known splicing junctions. 226688 novel splicing junctions.
sampling 75% (211447690) splicing reads. 440691 splicing junctions. 211560 known splicing junctions. 229131 novel splicing junctions.
sampling 80% (225544203) splicing reads. 443069 splicing junctions. 211825 known splicing junctions. 231244 novel splicing junctions.
sampling 85% (239640715) splicing reads. 445007 splicing junctions. 212011 known splicing junctions. 232996 novel splicing junctions.
sampling 90% (253737228) splicing reads. 446521 splicing junctions. 212167 known splicing junctions. 234354 novel splicing junctions.
sampling 95% (267833741) splicing reads. 447615 splicing junctions. 212273 known splicing junctions. 235342 novel splicing junctions.
sampling 100% (281930254) splicing reads. 448394 splicing junctions. 212364 known splicing junctions. 236030 novel splicing junctions.