Done. -parsing GTF file: /tmp/nxf.yGW5ZCT6lT/aih-tih-sc-2798bb-R1_A23WJ53LT4_1.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.yGW5ZCT6lT/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.yGW5ZCT6lT/aih-tih-sc-2798bb-R1_A23WJ53LT4_1_1.fastp.fastq.gz /tmp/nxf.yGW5ZCT6lT/aih-tih-sc-2798bb-R1_A23WJ53LT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.yGW5ZCT6lT/fi_workdir/aih-tih-sc-2798bb-R1_A23WJ53LT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.yGW5ZCT6lT/fi_workdir/aih-tih-sc-2798bb-R1_A23WJ53LT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam aih-tih-sc-2798bb-R1_A23WJ53LT4_1.star.sortedByCoord.out.bam * Running CMD: samtools index aih-tih-sc-2798bb-R1_A23WJ53LT4_1.star.sortedByCoord.out.bam 11:15:48 : INFO : Done. -parsing GTF file: /tmp/nxf.yGW5ZCT6lT/fi_workdir/aih-tih-sc-2798bb-R1_A23WJ53LT4_1.gtf -parsing /tmp/nxf.yGW5ZCT6lT/fi_workdir/aih-tih-sc-2798bb-R1_A23WJ53LT4_1.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] [90000] [100000] [110000] [120000] [130000] [140000] [150000] [160000] [170000] [180000] [190000] [200000] [210000] [220000] [230000] [240000] [250000] [260000] [270000] [280000] [290000] [300000] [310000] [320000] [330000] [340000] [350000] [360000] [370000] [380000] [390000] [400000] [410000] [420000] [430000] [440000] [450000] [460000] [470000] [480000] [490000] [500000] [510000] [520000] [530000] [540000] [550000] [560000] [570000] [580000] [590000] [600000] [610000] [620000] [630000] [640000] [650000] [660000] [670000] [680000] [690000] [700000] [710000] [720000] [730000] [740000] [750000] [760000] [770000] [780000] -done parsing /tmp/nxf.yGW5ZCT6lT/fi_workdir/aih-tih-sc-2798bb-R1_A23WJ53LT4_1.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { 'num_hits: 4 != num_counted_on_fusion_contigs 2 ' => 72, 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 51, 'low complexity anchor region' => 2, 'num_hits: 6 != num_counted_on_fusion_contigs 4 ' => 120, 'num genes matched < 2' => 11, 'per_id < 96' => 2765, 'excessive soft clipping' => 5269, ' ** passed ** ' => 1599, 'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 2, 'seq-similar region overlap' => 55 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.yGW5ZCT6lT/fi_workdir/aih-tih-sc-2798bb-R1_A23WJ53LT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] [103000] [104000] [105000] [106000] [107000] [108000] [109000] [110000] [111000] [112000] [113000] [114000] [115000] [116000] [117000] [118000] [119000] [120000] [121000] [122000] [123000] [124000] [125000] [126000] [127000] [128000] [129000] [130000] [131000] [132000] [133000] [134000] [135000] [136000] [137000] [138000] [139000] [140000] [141000] [142000] [143000] [144000] [145000] [146000] [147000] [148000] [149000] [150000] [151000] [152000] [153000] [154000] [155000] [156000] [157000] [158000] [159000] [160000] [161000] [162000] [163000] [164000] [165000] [166000] [167000] [168000] [169000] [170000] [171000] [172000] [173000] [174000] [175000] [176000] [177000] [178000] [179000] [180000] [181000] [182000] [183000] [184000] [185000] [186000] [187000] [188000] [189000] [190000] [191000] [192000] [193000] -fusion SPANNING read extraction for scaff: ATRX--AC074035.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] [103000] [104000] [105000] [106000] [107000] [108000] [109000] [110000] [111000] [112000] [113000] [114000] [115000] [116000] [117000] [118000] [119000] [120000] [121000] [122000] [123000] [124000] [125000] [126000] [127000] [128000] [129000] [130000] [131000] [132000] [133000] [134000] [135000] [136000] [137000] [138000] [139000] [140000] [141000] [142000] [143000] [144000] [145000] [146000] [147000] [148000] [149000] [150000] [151000] [152000] [153000] [154000] [155000] [156000] [157000] [158000] [159000] [160000] [161000] [162000] [163000] [164000] [165000] [166000] [167000] [168000] [169000] [170000] [171000] [172000] [173000] [174000] [175000] [176000] [177000] [178000] [179000] [180000] [181000] [182000] [183000] [184000] [185000] [186000] [187000] [188000] [189000] [190000] [191000] [192000] [193000] -fusion SPANNING read extraction for scaff: ATRX--MTND6P12 [1000] [2000] [3000] -fusion SPANNING read extraction for scaff: FAM86B1--ST18 [1000] [2000] [3000] -fusion SPANNING read extraction for scaff: FAM86B1--ALKAL1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] -fusion SPANNING read extraction for scaff: FSIP1--AC013652.1 [1000] -fusion SPANNING read extraction for scaff: KLRC4--KLRK1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] -fusion SPANNING read extraction for scaff: PCDHA10--PCDHAC1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] -fusion SPANNING read extraction for scaff: PCDHA10--PCDHA13 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] -fusion SPANNING read extraction for scaff: PCDHA10--PCDHAC2 [1000] [2000] [3000] [4000] [5000] [6000] -fusion SPANNING read extraction for scaff: PCDHA3--PCDHAC1 [1000] [2000] [3000] [4000] [5000] [6000] -fusion SPANNING read extraction for scaff: PCDHA3--PCDHA13 [1000] [2000] [3000] [4000] [5000] [6000] [7000] -fusion SPANNING read extraction for scaff: PCDHA3--PCDHAC2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] [103000] [104000] [105000] [106000] [107000] [108000] [109000] [110000] [111000] [112000] [113000] [114000] [115000] [116000] [117000] [118000] [119000] [120000] [121000] [122000] [123000] [124000] [125000] [126000] [127000] [128000] [129000] [130000] [131000] [132000] [133000] [134000] [135000] [136000] [137000] [138000] [139000] [140000] [141000] [142000] [143000] [144000] [145000] [146000] [147000] [148000] [149000] [150000] [151000] -fusion SPANNING read extraction for scaff: RPS5--ZNF71 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] -fusion SPANNING read extraction for scaff: STX16--NPEPL1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] -fusion SPANNING read extraction for scaff: TLK2--LINC01347 [1000] [2000] [3000] [4000] [5000] -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 -filtered reads reasons: $VAR1 = { 'seq similar region alignment' => 5653, 'lacks exon overlap' => 16798 }; EM: Starting log likelihood: -1665.681170 EM: Round [1] log likelihood: -1665.409400 EM: Round [2] log likelihood: -1665.332634 EM: Round [3] log likelihood: -1665.262463 EM: Round [4] log likelihood: -1665.198135 EM: Round [5] log likelihood: -1665.139055 EM: Round [6] log likelihood: -1665.084705 EM: Round [7] log likelihood: -1665.034630 EM: Round [8] log likelihood: -1664.988433 EM: Round [9] log likelihood: -1664.945759 EM: Round [10] log likelihood: -1664.906297 EM: Round [11] log likelihood: -1664.869766 EM: Round [12] log likelihood: -1664.835918 EM: Round [13] log likelihood: -1664.804529 EM: Round [14] log likelihood: -1664.775396 EM: Round [15] log likelihood: -1664.748340 EM: Round [16] log likelihood: -1664.723193 EM: Round [17] log likelihood: -1664.699809 EM: Round [18] log likelihood: -1664.678050 EM: Round [19] log likelihood: -1664.657794 EM: Round [20] log likelihood: -1664.638927 EM: Round [21] log likelihood: -1664.621346 EM: Round [22] log likelihood: -1664.604957 EM: Round [23] log likelihood: -1664.589673 EM: Round [24] log likelihood: -1664.575415 EM: Round [25] log likelihood: -1664.562110 EM: Round [26] log likelihood: -1664.549689 EM: Round [27] log likelihood: -1664.538091 EM: Round [28] log likelihood: -1664.527259 EM: Round [29] log likelihood: -1664.517139 EM: Round [30] log likelihood: -1664.507682 EM: Round [31] log likelihood: -1664.498843 EM: Round [32] log likelihood: -1664.490581 EM: Round [33] log likelihood: -1664.482855 EM: Round [34] log likelihood: -1664.475631 EM: Round [35] log likelihood: -1664.468873 EM: Round [36] log likelihood: -1664.462552 EM: Round [37] log likelihood: -1664.456638 EM: Round [38] log likelihood: -1664.451104 EM: Round [39] log likelihood: -1664.445925 EM: Round [40] log likelihood: -1664.441078 EM: Round [41] log likelihood: -1664.436541 EM: Round [42] log likelihood: -1664.432294 EM: Round [43] log likelihood: -1664.428318 EM: Round [44] log likelihood: -1664.424595 EM: Round [45] log likelihood: -1664.421109 EM: Round [46] log likelihood: -1664.417844 EM: Round [47] log likelihood: -1664.414787 EM: Round [48] log likelihood: -1664.411923 EM: Round [49] log likelihood: -1664.409241 EM: Round [50] log likelihood: -1664.406729 EM: Round [51] log likelihood: -1664.404376 EM: Round [52] log likelihood: -1664.402172 EM: Round [53] log likelihood: -1664.400106 EM: Round [54] log likelihood: -1664.398171 EM: Round [55] log likelihood: -1664.396359 EM: Round [56] log likelihood: -1664.394660 EM: Round [57] log likelihood: -1664.393069 EM: Round [58] log likelihood: -1664.391577 EM: Round [59] log likelihood: -1664.390180 EM: Round [60] log likelihood: -1664.388871 EM: Round [61] log likelihood: -1664.387644 EM: Round [62] log likelihood: -1664.386494 EM: Round [63] log likelihood: -1664.385416 EM: Round [64] log likelihood: -1664.384406 EM: Round [65] log likelihood: -1664.383460 EM: Round [66] log likelihood: -1664.382572 EM: Round [67] log likelihood: -1664.381741 EM: Round [68] log likelihood: -1664.380962 EM: Round [69] log likelihood: -1664.380231 EM: Round [70] log likelihood: -1664.379547 EM: Round [71] log likelihood: -1664.378905 EM: Round [72] log likelihood: -1664.378304 EM: Round [73] log likelihood: -1664.377740 EM: Round [74] log likelihood: -1664.377212 EM: Round [75] log likelihood: -1664.376717 EM: Round [76] log likelihood: -1664.376252 EM: Round [77] log likelihood: -1664.375817 EM: Round [78] log likelihood: -1664.375409 EM: Round [79] log likelihood: -1664.375027 EM: Round [80] log likelihood: -1664.374668 EM: Round [81] log likelihood: -1664.374332 EM: Round [82] log likelihood: -1664.374018 EM: Round [83] log likelihood: -1664.373722 EM: Round [84] log likelihood: -1664.373446 EM: Round [85] log likelihood: -1664.373186 EM: Round [86] log likelihood: -1664.372943 EM: Round [87] log likelihood: -1664.372715 EM: Round [88] log likelihood: -1664.372501 EM: Round [89] log likelihood: -1664.372301 EM: Round [90] log likelihood: -1664.372113 EM: Round [91] log likelihood: -1664.371937 EM: Round [92] log likelihood: -1664.371772 EM: Round [93] log likelihood: -1664.371617 EM: Round [94] log likelihood: -1664.371472 EM: Round [95] log likelihood: -1664.371336 EM: Round [96] log likelihood: -1664.371209 EM: Round [97] log likelihood: -1664.371089 EM: Round [98] log likelihood: -1664.370977 EM: Round [99] log likelihood: -1664.370872 EM: Round [100] log likelihood: -1664.370774 EM: Stopping iterations at round 100 due to insufficient improvement in likelihood. [bam_sort_core] merging from 0 files and 8 in-memory blocks... -extracting read coordinates from /tmp/nxf.yGW5ZCT6lT/aih-tih-sc-2798bb-R1_A23WJ53LT4_1.consolidated.bam into /tmp/nxf.yGW5ZCT6lT/aih-tih-sc-2798bb-R1_A23WJ53LT4_1.consolidated.bam.read_coords CMD: touch /tmp/nxf.yGW5ZCT6lT/aih-tih-sc-2798bb-R1_A23WJ53LT4_1.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.yGW5ZCT6lT/aih-tih-sc-2798bb-R1_A23WJ53LT4_1.consolidated.bam.read_coords > /tmp/nxf.yGW5ZCT6lT/aih-tih-sc-2798bb-R1_A23WJ53LT4_1.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.yGW5ZCT6lT/aih-tih-sc-2798bb-R1_A23WJ53LT4_1.consolidated.bam.frag_coords > /tmp/nxf.yGW5ZCT6lT/aih-tih-sc-2798bb-R1_A23WJ53LT4_1.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.yGW5ZCT6lT/fi_workdir/aih-tih-sc-2798bb-R1_A23WJ53LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.yGW5ZCT6lT/fi_workdir/aih-tih-sc-2798bb-R1_A23WJ53LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.yGW5ZCT6lT/fi_workdir/aih-tih-sc-2798bb-R1_A23WJ53LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.yGW5ZCT6lT/aih-tih-sc-2798bb-R1_A23WJ53LT4_1.post_blast_and_promiscuity_filter -done, see /tmp/nxf.yGW5ZCT6lT/aih-tih-sc-2798bb-R1_A23WJ53LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -1305.990832 EM: Round [1] log likelihood: -1305.986341 EM: Round [2] log likelihood: -1305.986336 EM: Stopping iterations at round 2 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.yGW5ZCT6lT/aih-tih-sc-2798bb-R1_A23WJ53LT4_1_1.fastp.fastq.gz: 296427168 Warning - not locating file: /tmp/nxf.yGW5ZCT6lT/aih-tih-sc-2798bb-R1_A23WJ53LT4_1.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000226049.3] WARNING, no entry stored in dbm for [ENSG00000260404.3] WARNING, no entry stored in dbm for [ENSG00000214837.8] WARNING, no entry stored in dbm for [ENSG00000259345.7] WARNING, no entry stored in dbm for [ENSG00000259345.7] WARNING, no entry stored in dbm for [ENSG00000259345.7] WARNING, no entry stored in dbm for [ENSG00000259345.7] ls: cannot access 'IGV_inputs': No such file or directory