[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2ca847-R1_A23WJ53LT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	167916303
Known Splicing Events:	166459042
Partial Novel Splicing Events:	893822
Novel Splicing Events:	498160
Filtered Splicing Events:	65279

Total splicing  Junctions:	351460
Known Splicing Junctions:	227993
Partial Novel Splicing Junctions:	74264
Novel Splicing Junctions:	49203

===================================================================
Create BED file ...
Create Interact file ...