+ mkdir FFPE_V4_0002_RNA_0006_A23WJ53LT4_1 + all_qc -bam FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder FFPE_V4_0002_RNA_0006_A23WJ53LT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai 0it [00:00, ?it/s] 150182it [00:00, 1501753.40it/s] 308951it [00:00, 1552034.39it/s] 470984it [00:00, 1583194.64it/s] 637353it [00:00, 1614869.81it/s] 804137it [00:00, 1633949.10it/s] 968845it [00:00, 1638375.35it/s] 1132683it [00:00, 1633990.06it/s] 1296920it [00:00, 1636634.75it/s] 1460681it [00:00, 1636923.88it/s] 1625054it [00:01, 1638846.50it/s] 1790300it [00:01, 1642994.92it/s] 1954601it [00:01, 1642874.93it/s] 2118890it [00:01, 1633816.22it/s] 2289863it [00:01, 1656637.31it/s] 2457168it [00:01, 1661556.47it/s] 2623647it [00:01, 1662513.97it/s] 2789909it [00:01, 1661273.73it/s] 2956044it [00:01, 1657872.16it/s] 3121838it [00:01, 1648463.95it/s] 3286699it [00:02, 1634388.86it/s] 3451289it [00:02, 1637793.72it/s] 3617861it [00:02, 1646030.95it/s] 3786209it [00:02, 1657196.79it/s] 3951951it [00:02, 1649632.86it/s] 4117467it [00:02, 1651268.19it/s] 4282610it [00:02, 1650752.48it/s] 4447706it [00:02, 1650797.36it/s] 4612794it [00:02, 1642260.91it/s] 4777034it [00:02, 1640912.16it/s] 4941989it [00:03, 1643394.87it/s] 5106336it [00:03, 1627223.90it/s] 5272621it [00:03, 1637788.33it/s] 5441011it [00:03, 1651504.40it/s] 5610241it [00:03, 1663659.57it/s] 5779059it [00:03, 1670840.69it/s] 5946225it [00:03, 1671063.74it/s] 6115597it [00:03, 1677825.14it/s] 6284883it [00:03, 1682306.98it/s] 6453123it [00:03, 1676501.44it/s] 6620784it [00:04, 1672161.67it/s] 6788009it [00:04, 1663954.12it/s] 6954888it [00:04, 1665379.80it/s] 7121767it [00:04, 1666383.46it/s] 7288413it [00:04, 1663977.99it/s] 7454816it [00:04, 1663928.77it/s] 7622059it [00:04, 1666387.08it/s] 7788701it [00:04, 1660861.02it/s] 7954793it [00:04, 1654852.76it/s] 8120690it [00:04, 1656066.96it/s] 8287474it [00:05, 1659569.30it/s] 8457203it [00:05, 1670841.61it/s] 8625642it [00:05, 1674885.26it/s] 8793136it [00:05, 1662653.72it/s] 8959425it [00:05, 1641298.59it/s] 9123622it [00:05, 1639968.77it/s] 9291906it [00:05, 1652688.70it/s] 9459806it [00:05, 1660513.27it/s] 9626912it [00:05, 1663648.03it/s] 9796436it [00:05, 1673074.36it/s] 9965910it [00:06, 1679543.21it/s] 10137053it [00:06, 1689031.93it/s] 10305970it [00:06, 1681022.97it/s] 10474090it [00:06, 1662589.91it/s] 10640400it [00:06, 1656356.16it/s] 10806071it [00:06, 1650518.45it/s] 10974482it [00:06, 1660482.82it/s] 11140557it [00:06, 1657971.64it/s] 11306372it [00:06, 1656037.56it/s] 11471988it [00:06, 1648090.03it/s] 11636811it [00:07, 1639331.82it/s] 11802834it [00:07, 1645435.11it/s] 11967393it [00:07, 1638391.61it/s] 12135178it [00:07, 1649956.42it/s] 12302104it [00:07, 1655703.95it/s] 12467690it [00:07, 1654513.00it/s] 12635919it [00:07, 1662805.65it/s] 12806162it [00:07, 1674652.33it/s] 12973637it [00:07, 1670081.71it/s] 13140654it [00:07, 1666864.69it/s] 13307348it [00:08, 1666724.92it/s] 13474026it [00:08, 1660871.40it/s] 13640120it [00:08, 1658923.88it/s] 13722355it [00:08, 1653471.10it/s] [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md.bai + merge_qc -folders FFPE_V4_0002_RNA_0006_A23WJ53LT4_1/FFPE_V4_0002_RNA_0006_A23WJ53LT4_1.md -output FFPE_V4_0002_RNA_0006_A23WJ53LT4_1/FFPE_V4_0002_RNA_0006_A23WJ53LT4_1_full_basic_stats.tsv + head -n1 FFPE_V4_0002_RNA_0006_A23WJ53LT4_1/FFPE_V4_0002_RNA_0006_A23WJ53LT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed FFPE_V4_0002_RNA_0006_A23WJ53LT4_1/FFPE_V4_0002_RNA_0006_A23WJ53LT4_1_full_basic_stats.tsv