[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1dd7a6-R1_A23WJ53LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (16667364) splicing reads. 262027 splicing junctions. 187379 known splicing junctions. 74648 novel splicing junctions.
sampling 10% (33334729) splicing reads. 313022 splicing junctions. 198695 known splicing junctions. 114327 novel splicing junctions.
sampling 15% (50002093) splicing reads. 345986 splicing junctions. 204538 known splicing junctions. 141448 novel splicing junctions.
sampling 20% (66669458) splicing reads. 370094 splicing junctions. 208230 known splicing junctions. 161864 novel splicing junctions.
sampling 25% (83336822) splicing reads. 388345 splicing junctions. 210704 known splicing junctions. 177641 novel splicing junctions.
sampling 30% (100004187) splicing reads. 402849 splicing junctions. 212643 known splicing junctions. 190206 novel splicing junctions.
sampling 35% (116671551) splicing reads. 414570 splicing junctions. 214070 known splicing junctions. 200500 novel splicing junctions.
sampling 40% (133338916) splicing reads. 424201 splicing junctions. 215221 known splicing junctions. 208980 novel splicing junctions.
sampling 45% (150006280) splicing reads. 432173 splicing junctions. 216138 known splicing junctions. 216035 novel splicing junctions.
sampling 50% (166673645) splicing reads. 438820 splicing junctions. 216825 known splicing junctions. 221995 novel splicing junctions.
sampling 55% (183341009) splicing reads. 444553 splicing junctions. 217444 known splicing junctions. 227109 novel splicing junctions.
sampling 60% (200008374) splicing reads. 449393 splicing junctions. 217971 known splicing junctions. 231422 novel splicing junctions.
sampling 65% (216675738) splicing reads. 453368 splicing junctions. 218374 known splicing junctions. 234994 novel splicing junctions.
sampling 70% (233343103) splicing reads. 456769 splicing junctions. 218732 known splicing junctions. 238037 novel splicing junctions.
sampling 75% (250010467) splicing reads. 459667 splicing junctions. 219021 known splicing junctions. 240646 novel splicing junctions.
sampling 80% (266677832) splicing reads. 462135 splicing junctions. 219252 known splicing junctions. 242883 novel splicing junctions.
sampling 85% (283345196) splicing reads. 464169 splicing junctions. 219446 known splicing junctions. 244723 novel splicing junctions.
sampling 90% (300012561) splicing reads. 465836 splicing junctions. 219614 known splicing junctions. 246222 novel splicing junctions.
sampling 95% (316679925) splicing reads. 467083 splicing junctions. 219708 known splicing junctions. 247375 novel splicing junctions.
sampling 100% (333347290) splicing reads. 467998 splicing junctions. 219800 known splicing junctions. 248198 novel splicing junctions.